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Volume 65, Issue Pt_4

sp. nov., an endophytic actinomycete isolated from the roots of a jambolan plum tree ( L. Skeels) Free

Abstract

A novel endophytic actinomycete, designated strain GKU 164, was isolated from the roots of a jambolan plum tree ( L. Skeels), collected at Khao Khitchakut National Park, Chantaburi province, Thailand. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain formed a distinct clade within the genus , and was most closely related to PT708 (98.77 % 16S rRNA gene sequence similarity) and KC-061 (98.73 %). Strain GKU 164 formed a branched substrate and aerial hyphae that generated single spores with rough surfaces. The cell wall contained -diaminopimelic acid. The whole-cell sugars were madurose, galactose, mannose, ribose, rhamnose and glucose. The -acyl type of muramic acid was acetyl. The predominant menaquinone was MK-9(H) with minor amounts of MK-9(H), MK-9(H) and MK-9(H). The phospholipid profile contained diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositolmannosides, phosphatidylmonomethylethanolamine, hydroxy-phosphatidylmonomethylethanolamine, an unidentified aminophosphoglycolipid and four unknown phospholipids. The major fatty acids were iso-C and 10-methyl C. The genomic DNA G+C content was 70.4 mol%. Significant differences in the morphological, chemotaxonomical, and biochemical data together with DNA–DNA relatedness values between strain GKU 164 and type strains of closely related species, clearly demonstrated that strain GKU 164 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is GKU 164 ( = BCC 70457 = NBRC 110400).

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Funding
This study was supported by the:
  • Thailand Research Fund (TRF) (Award DBG5480002)
  • Kitasato Institute for Life Sciences, Kitasato University
© 2015 IUMS
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2015-04-01
2024-04-18
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Nonomuraea syzygii sp. nov., an endophytic actinomycete isolated from the roots of a jambolan plum tree (Syzygium cumini L. Skeels)
Int J Syst Evol Microbiol 65, 1234 (2015); https://doi.org/10.1099/ijs.0.000085
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