1887

Abstract

A novel endophytic actinomycete, designated strain GKU 164, was isolated from the roots of a jambolan plum tree ( L. Skeels), collected at Khao Khitchakut National Park, Chantaburi province, Thailand. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain formed a distinct clade within the genus , and was most closely related to PT708 (98.77 % 16S rRNA gene sequence similarity) and KC-061 (98.73 %). Strain GKU 164 formed a branched substrate and aerial hyphae that generated single spores with rough surfaces. The cell wall contained -diaminopimelic acid. The whole-cell sugars were madurose, galactose, mannose, ribose, rhamnose and glucose. The -acyl type of muramic acid was acetyl. The predominant menaquinone was MK-9(H) with minor amounts of MK-9(H), MK-9(H) and MK-9(H). The phospholipid profile contained diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositolmannosides, phosphatidylmonomethylethanolamine, hydroxy-phosphatidylmonomethylethanolamine, an unidentified aminophosphoglycolipid and four unknown phospholipids. The major fatty acids were iso-C and 10-methyl C. The genomic DNA G+C content was 70.4 mol%. Significant differences in the morphological, chemotaxonomical, and biochemical data together with DNA–DNA relatedness values between strain GKU 164 and type strains of closely related species, clearly demonstrated that strain GKU 164 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is GKU 164 ( = BCC 70457 = NBRC 110400).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000085
2015-04-01
2019-10-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/4/1234.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000085&mimeType=html&fmt=ahah

References

  1. Atlas R. M. . ( 2004; ). Handbook of Microbiological Media, , 3rd edn.. Florida:: CRC Press;. [CrossRef]
    [Google Scholar]
  2. Becker B. , Lechevalier M. P. , Lechevalier H. A. . ( 1965; ). Chemical composition of cell-wall preparations from strains of various form-genera of aerobic actinomycetes. . Appl Microbiol 13:, 236–243.[PubMed]
    [Google Scholar]
  3. Chiba S. , Suzuki M. , Ando K. . ( 1999; ). Taxonomic re-evaluation of ‘Nocardiopsis’ sp. K-252T ( = NRRL 15532T): a proposal to transfer this strain to the genus Nonomuraea as Nonomuraea longicatena sp. nov.. Int J Syst Bacteriol 49:, 1623–1630. [CrossRef] [PubMed]
    [Google Scholar]
  4. Collins M. D. , Pirouz T. , Goodfellow M. , Minnikin D. E. . ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100:, 221–230. [CrossRef] [PubMed]
    [Google Scholar]
  5. Ezaki T. , Hashimoto Y. , Yabuuchi E. . ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  6. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  7. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  8. Gordon R. E. , Barnett D. A. , Handerhan J. E. , Pang C. H.-N. . ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol 24:, 54–63. [CrossRef]
    [Google Scholar]
  9. Hasegawa T. , Takizawa M. , Tanida S. . ( 1983; ). A rapid analysis for chemical grouping of aerobic actinomycetes. . J Gen Appl Microbiol 29:, 319–322. [CrossRef]
    [Google Scholar]
  10. Hobbs G. , Frazer C. M. , Gardner D. C. J. , Cullum J. A. , Oliver S. G. . ( 1989; ). Dispersed growth of Streptomyces in liquid culture. . Appl Microbiol Biotechnol 31:, 272–277. [CrossRef]
    [Google Scholar]
  11. Indananda C. , Matsumoto A. , Inahashi Y. , Takahashi Y. , Duangmal K. , Thamchaipenet A. . ( 2010; ). Actinophytocola oryzae gen. nov., sp. nov., isolated from the roots of Thai glutinous rice plants, a new member of the family Pseudonocardiaceae . . Int J Syst Evol Microbiol 60:, 1141–1146. [CrossRef] [PubMed]
    [Google Scholar]
  12. Jacobson E. , Grauville W. C. , Fogs C. E. . ( 1958; ). Color Harmony Manual, , 4th edn.. Chicago:: Container Corporation of America;.
    [Google Scholar]
  13. Jones K. L. . ( 1949; ). Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. . J Bacteriol 57:, 141–145.[PubMed]
    [Google Scholar]
  14. Kämpfer P. , Kroppenstedt R. M. , Grün-Wollny I. . ( 2005; ). Nonomuraea kuesteri sp. nov.. Int J Syst Evol Microbiol 55:, 847–851. [CrossRef] [PubMed]
    [Google Scholar]
  15. Kataoka M. , Ueda K. , Kudo T. , Seki T. , Yoshida T. . ( 1997; ). Application of the variable region in 16S rDNA to create an index for rapid species identification in the genus Streptomyces . . FEMS Microbiol Lett 151:, 249–255. [CrossRef] [PubMed]
    [Google Scholar]
  16. Kieser Y. , Bibb M. J. , Buttner M. J. , Chater K. F. , Hopwood D. A. . ( 2000; ). Practical Streptomyces Genetics. Norwich:: The John Innes Foundation;.
    [Google Scholar]
  17. Kim O.-S. , Cho Y.-J. , Lee K. , Yoon S.-H. , Kim M. , Na H. , Park S.-C. , Jeon Y. S. , Lee J.-H. et al. ( 2012; ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  18. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  19. Küster E. , Williams S. T. . ( 1964; ). Selection of media for isolation of streptomycetes. . Nature 202:, 928–929. [CrossRef] [PubMed]
    [Google Scholar]
  20. Larkin M. A. , Blackshields G. , Brown N. P. , Chenna R. , McGettigan P. A. , McWilliam H. , Valentin F. , Wallace I. M. , Wilm A. et al. ( 2007; ). clustal w and clustal x version 2.0. . Bioinformatics 23:, 2947–2948. [CrossRef] [PubMed]
    [Google Scholar]
  21. Lechevalier M. P. , Lechevalier H. A. . ( 1970; ). Chemical composition as a criterion in the classification of aerobic actinomycetes. . Int J Syst Bacteriol 20:, 435–443. [CrossRef]
    [Google Scholar]
  22. Lechevalier M. P. , De Bièvre C. , Lechevalier H. . ( 1977; ). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. . Biochem Syst Ecol 5:, 249–260. [CrossRef]
    [Google Scholar]
  23. Li J. , Zhao G.-Z. , Huang H.-Y. , Zhu W.-Y. , Lee J.-C. , Xu L.-H. , Kim C.-J. , Li W.-J. . ( 2011; ). Nonomuraea endophytica sp. nov., an endophytic actinomycete isolated from Artemisia annua L.. Int J Syst Evol Microbiol 61:, 757–761. [CrossRef] [PubMed]
    [Google Scholar]
  24. Marmur J. . ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  25. Minnikin D. E. , O’Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  26. Nakaew N. , Sungthong R. , Yokota A. , Lumyong S. . ( 2012; ). Nonomuraea monospora sp. nov., an actinomycete isolated from cave soil in Thailand, and emended description of the genus Nonomuraea . . Int J Syst Evol Microbiol 62:, 3007–3012. [CrossRef] [PubMed]
    [Google Scholar]
  27. Nonomura H. , Ohara Y. . ( 1971; ). Distribution of actinomycetes in soil. XI. Some new species of the genus Actinomadura Lechevalier et al.. J Ferment Technol 49:, 904–912.
    [Google Scholar]
  28. Ozdemir-Kocak F. , Isik K. , Veyisoglu A. , Tatar D. , Sahin N. . ( 2014; ). Nonomuraea muscovyensis sp. nov., isolated from soil. . Int J Syst Evol Microbiol 64:, 2467–2472. [CrossRef] [PubMed]
    [Google Scholar]
  29. Qin S. , Zhao G.-Z. , Klenk H.-P. , Li J. , Zhu W.-Y. , Xu L.-H. , Li W.-J. . ( 2009; ). Nonomuraea antimicrobica sp. nov., an endophytic actinomycete isolated from a leaf of Maytenus austroyunnanensis . . Int J Syst Evol Microbiol 59:, 2747–2751.[PubMed] [CrossRef]
    [Google Scholar]
  30. Quintana E. , Maldonado L. , Goodfellow M. . ( 2003; ). Nonomuraea terrinata sp. nov., a novel soil actinomycete. . Antonie van Leeuwenhoek 84:, 1–6. [CrossRef] [PubMed]
    [Google Scholar]
  31. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  32. Sasser M . . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  33. Shirling E. B. , Gottlieb D. . ( 1966; ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16:, 313–340. [CrossRef]
    [Google Scholar]
  34. Sripreechasak P. , Tanasupawat S. , Suwanborirux K. , Inahashi Y. , Matsumoto A. , Shiomi K. , Takahashi Y. . ( 2013; ). Nonomuraea thailandensis sp. nov. isolated from Thai soil. . J Antibiot (Tokyo) 66:, 79–84. [CrossRef] [PubMed]
    [Google Scholar]
  35. Stuart C. A. , Van Stratum E. , Rustigian R. . ( 1945; ). Further studies on urease production by proteus and related organisms. . J Bacteriol 49:, 437–444.[PubMed]
    [Google Scholar]
  36. Tajima K. , Takahashi Y. , Seino A. , Iwai Y. , Omura S. . ( 2001; ). Description of two novel species of the genus Kitasatospora Omura et al. 1982, Kitasatospora cineracea sp. nov. and Kitasatospora niigatensis sp. nov.. Int J Syst Evol Microbiol 51:, 1765–1771. [CrossRef] [PubMed]
    [Google Scholar]
  37. Tamaoka J. , Komagata K. . ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  38. Tamura K. , Stecher G. , Peterson D. , Filipski A. , Kumar S. . ( 2013; ). mega6: molecular evolutionary genetics analysis version 6.0. . Mol Biol Evol 30:, 2725–2729. [CrossRef] [PubMed]
    [Google Scholar]
  39. Tomiyasu I. . ( 1982; ). Mycolic acid composition and thermally adaptative changes in Nocardia asteroides . . J Bacteriol 151:, 828–837.[PubMed]
    [Google Scholar]
  40. Uchida K. , Aida K. . ( 1984; ). An improved method for the glycolate test for simple identification of acyl type of bacterial cell walls. . J Gen Appl Microbiol 30:, 131–134. [CrossRef]
    [Google Scholar]
  41. Wang F. , Xu X.-X. , Qu Z. , Wang C. , Lin H.-P. , Xie Q.-Y. , Ruan J.-S. , Sun M. , Hong K. . ( 2011; ). Nonomuraea wenchangensis sp. nov., isolated from mangrove rhizosphere soil. . Int J Syst Evol Microbiol 61:, 1304–1308. [CrossRef] [PubMed]
    [Google Scholar]
  42. Wang X. , Zhao J. , Liu C. , Wang J. , Shen Y. , Jia F. , Wang L. , Zhang J. , Yu C. , Xiang W. . ( 2013; ). Nonomuraea solani sp. nov., an actinomycete isolated from eggplant root (Solanum melongena L.). . Int J Syst Evol Microbiol 63:, 2418–2423. [CrossRef] [PubMed]
    [Google Scholar]
  43. Wang S. , Liu C. , Zhang Y. , Zhao J. , Zhang X. , Yang L. , Wang X. , Xiang W. . ( 2014; ). Nonomuraea guangzhouensis sp. nov., and Nonomuraea harbinensis sp. nov., two novel actinomycetes isolated from soil. . Antonie van Leeuwenhoek 105:, 109–118. [CrossRef] [PubMed]
    [Google Scholar]
  44. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. et al. ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  45. Xi L. , Zhang L. , Ruan J. , Huang Y. . ( 2011; ). Nonomuraea maritima sp. nov., isolated from coastal sediment. . Int J Syst Evol Microbiol 61:, 2740–2744. [CrossRef] [PubMed]
    [Google Scholar]
  46. Zhang Z. , Wang Y. , Ruan J. . ( 1998; ). Reclassification of Thermomonospora and Microtetraspora . . Int J Syst Bacteriol 48:, 411–422. [CrossRef] [PubMed]
    [Google Scholar]
  47. Zhang X. , Zhang Y. , Zhao J. , Liu C. , Wang S. , Yang L. , He H. , Xiang W. , Wang X. . ( 2014; a). Nonomuraea fuscirosea sp. nov., an actinomycete isolated from the rhizosphere soil of rehmannia (Rehmannia glutinosa Libosch). . Int J Syst Evol Microbiol 64:, 1102–1107. [CrossRef] [PubMed]
    [Google Scholar]
  48. Zhang Y. , Zhao J. , Liu C. , Shen Y. , Jia F. , Wang X. , Xiang W. . ( 2014; b). Nonomuraea shaanxiensis sp. nov., a novel actinomycete isolated from a soil sample. . Antonie van Leeuwenhoek 105:, 57–64. [CrossRef] [PubMed]
    [Google Scholar]
  49. Zhao G.-Z. , Li J. , Huang H.-Y. , Zhu W.-Y. , Xu L.-H. , Li W.-J. . ( 2011; ). Nonomuraea rhizophila sp. nov., an actinomycete isolated from rhizosphere soil. . Int J Syst Evol Microbiol 61:, 2141–2145. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000085
Loading
/content/journal/ijsem/10.1099/ijs.0.000085
Loading

Data & Media loading...

Supplementary Data



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error