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Abstract

Strain SK9K4, which is a strictly anaerobic, non-motile, non-sporulating, Gram-stain-positive, saccharolytic coccobacillus, was isolated from pig faeces. SK9K4 metabolized indol-3-acetic acid to 3-methylindole (skatole), which is the main contributor to boar taint; it also produced 4-methylphenol (-cresol) from -hydroxyphenylacetic acid. Phylogenetic analyses, based on 16S rRNA gene sequences, revealed that the isolate represented a new lineage within the genus of the family . Strain SK9K4 was most closely related to the type strains of the three species of the genus with validly published names; DSM 13989 (93.6 %), DSM 7084 (93.5 %) and DSM 22620 (92.7 %). DNA–DNA relatedness values of strain SK9K4 with , and were 28.3 %, 69.1 % and 27.2 %, respectively. The genomic DNA G+C content was 62.1 mol% and the major cellular fatty acids (constituting >10 % of the total) were C and Cω9. The major end product of glucose fermentation was lactic acid, with minor amounts of acetic acid and formic acid; no H was produced. Discrepancies in the fatty acid profiles, the MALDI-TOF mass spectra of cell extracts and the physiological and biochemical characteristics differentiated strain SK9K4 from other species of the genus and indicate that the isolate represents a novel species within this genus. The name sp. nov., is proposed and the type strain is SK9K4 ( = JCM 19907 = DSM 28304).

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2015-04-01
2020-01-26
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