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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 65, Issue Pt_4

Revision of the taxonomic status of the species and reclassification as comb. nov. Free

Abstract

The species was isolated from nodules and included in the Approved Lists of Bacterial Names in 1980. Nevertheless, on the basis of the analysis of the type strain of this species available in DSMZ, DSM 30140, whose 16S rRNA gene was identical to that of the type strain of , was considered a later synonym of this species. In this study we confirmed that the strain DSM 30140 belongs to the species , but also that it cannot be the original strain of because this species effectively nodulated whereas strain DSM 30140 was able to nodulate soybean but not . Since the original type strain of was deposited into the USDA collection by L. W. Erdman under the accession number USDA 3051 we analysed the taxonomic status of this strain showing that although it belongs to the genus instead of genus , it is phylogenetically distant from and closely related to . The type strains USDA 3051 and BTA-1 share 16S rRNA, and gene sequences with similarities of 99.8 %, 96.5 % and 97.1 %, respectively. They presented a DNA–DNA hybridization value of 36 % and also differed in phenotypic characteristics and slightly in the proportions of some fatty acids. Therefore we propose the reclassification of the species as comb. nov. The type strain is USDA 3051 ( = CECT 8630 = LMG 28514).

  • Published Online:
Funding
This study was supported by the:
  • JCYL
  • MICINN
  • Spanish Governments
  • CSIC
  • Universidad de Salamanca
© 2015 IUMS
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2015-04-01
2024-04-25
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Revision of the taxonomic status of the species Rhizobium lupini and reclassification as Bradyrhizobium lupini comb. nov.
Int J Syst Evol Microbiol 65, 1213 (2015); https://doi.org/10.1099/ijs.0.000082
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