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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 65, Issue Pt_4

sp. nov., an endophytic actinobacterium isolated from the root of (C. A. Mey.) Schischk Free

Abstract

A novel endophytic actinobacterium, designated EGI 6500707, was isolated from the surface-sterilized root of a halophyte (C. A. Mey.) Schischk collected from Urumqi, Xinjiang province, north-west China, and characterized using a polyphasic approach. Cells were Gram-stain-positive, non-motile, short rods and produced white colonies. Growth occurred at 10–45 °C (optimum 25–30 °C), at pH 5–10 (optimum pH 8) and in presence of 0–4 % (w/v) NaCl (optimum 0–3 %). The predominant menaquinone was MK-9. The diagnostic phospholipids were diphosphatidylglycerol and phosphatidylglycerol. The major fatty acids were anteiso-C, anteiso-C and iso-C. The DNA G+C content of strain EGI 6500707 was 69.1 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain EGI 6500707 should be placed in the genus (family , phylum ), and that the novel strain exhibited the highest 16S rRNA gene sequence similarity to JCM 11265 (99.1 %) and MSL-08 (96.5 %). DNA–DNA relatedness between strain EGI 6500707 and JCM 11265 was 47.2 %. On the basis of phenotypic and chemotaxonomic characteristics, phylogenetic analysis and DNA–DNA relatedness data, strain EGI 6500707 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is EGI 6500707 ( = JCM 30093 = KCTC 29493).

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31200008 and 31400009)
  • Hundred Talents Program of Chinese Academy of Sciences
  • High-level Talents Program of Xinjiang Autonomous Region
  • West Light Foundation of Chinese Academy of Sciences
  • Deanship of Scientific Research at King Saud University (Award RGP-205)
  • Guangdong Province Higher Vocational Colleges & Schools Pearl River Scholar Funded Scheme
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2015-04-01
2025-01-22
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Frigoribacterium endophyticum sp. nov., an endophytic actinobacterium isolated from the root of Anabasis elatior (C. A. Mey.) Schischk
Int J Syst Evol Microbiol 65, 1207 (2015); https://doi.org/10.1099/ijs.0.000081
/content/journal/ijsem/10.1099/ijs.0.000081
/content/journal/ijsem/10.1099/ijs.0.000081
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