1887

Abstract

A Gram-stain-negative, aerobic, rod-shaped and motile bacterium, designated THG-DD7, was isolated from tomato plant rhizosphere soil. Strain THG-DD7 grew optimally at 25–30 °C, at pH 7.0–7.5 and in the presence of 0.5 % (w/v) NaCl. According to the results of 16S rRNA gene sequence comparisons, strain THG-DD7 was most closely related to GR24-2 (98.2 %), LnR5-47 (98.0 %), DCY45 (97.9 %), GP18-1 (97.9 %) and Gsoil 3046 (97.7 %). The DNA G+C content was 65.2 mol%. In DNA–DNA hybridization, the DNA relatedness levels between strain THG-DD7 and its closest phylogenetically neighbours were below 40.0 %. The predominant isoprenoid quinone was ubiquinone Q-8. The major polar lipids were diphosphatidylglycerol, phosphtidylethanolamine, phosphatidyl--methylethanolamine and phosphatidylglycerol. The major fatty acids were iso-C, iso-C, iso-C, anteiso-C and iso-Cω9 and/or C 10-methyl (summed feature 9). These data supported the affiliation of strain THG-DD7 to the genus . The results of physiological and biochemical tests enabled strain THG-DD7 to be differentiated genotypically and phenotypically from the species of the genus with validly published names. Therefore, the novel isolate represents a novel species, for which the name sp. nov. is proposed. The type strain is THG-DD7 ( = KACC 17650 = JCM 19614).

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2015-04-01
2020-01-22
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