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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 65, Issue Pt_4

sp. nov., isolated from roots of Free

Abstract

A yellow-pigmented bacterium, designated strain ZFGT-11, was isolated from roots of Jacq. collected from Taibai Mountain in Shaanxi Province, north-west China, and was subjected to a taxonomic study by using a polyphasic approach. Cells of strain ZFGT-11 were Gram-stain-negative, strictly aerobic rods that were surrounded by a thick capsule and were motile by means of a single polar flagellum. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain ZFGT-11 was a member of the genus and was closely related to KACC 16534 (97.6 % similarity), JCM 18825 (96.8 %), IFO 15499 (96.7 %) and DSM 4733 (96.6 %). The predominant respiratory quinone was ubiquinone-10 (Q-10) and the major cellular fatty acids were summed feature 8 (comprising Cω7 and/or Cω6), Cω6, C 2-OH, C and C 2-OH. The major polyamine of strain ZFGT-11 was -homospermidine. Phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylcholine, sphingoglycolipid, two unidentified aminoglycolipids, two unidentified phospholipids and two unidentified lipids were detected in the polar lipid profile. The DNA G+C content was 66.8 mol%. DNA–DNA relatedness for strain ZFGT-11 with respect to its closest phylogenetic relative KACC 16534 was 26.2±4.8 % (mean±). On the basis of data from the present polyphasic taxonomic study, strain ZFGT-11 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is ZFGT-11 ( = CCTCC AB 2013306 = KCTC 32449 = LMG 27608).

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Funding
This study was supported by the:
  • National High Technology Research and Development Program of China (Award 2013AA102802)
  • National Natural Science Foundation of China (Award 31100001 and 31300158)
  • Natural Science Foundation of Shaanxi Province, China (Award 2012JQ3006)
  • Fundamental Research Funds for the Central Universities (Award QN2012020)
  • Specialized Research Fund for the Doctoral Program of Higher Education of China (Award 20120204120032)
© 2015 IUMS
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2015-04-01
2025-01-21
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Sphingomonas gei sp. nov., isolated from roots of Geum aleppicum
Int J Syst Evol Microbiol 65, 1160 (2015); https://doi.org/10.1099/ijs.0.000074
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