1887

Abstract

A Gram-stain-negative, facultatively anaerobic, chemoheterotrophic, moderately halophilic, exopolysaccharide (EPS)-producing, cream, non-motile and rod-shaped bacterium, designated strain ZH114, was isolated from deep water of the South China Sea, and was subjected to a polyphasic taxonomic study. Phylogenetic analysis, based on 16S rRNA gene sequences, indicated that this strain belongs to the genus with the highest sequence similarity to LMG 22090 (96.83 %), followed by LMG 24574 (96.12 %). Growth occurred at 4–37 °C (optimum 32 °C), pH 6.0–10.0 (optimum pH 9.0–10.0) and in the presence of 0–19 % NaCl (w/v) (optimum 6 %, w/v). It did not produce poly-β-hydroxyalkanoate granules or bacteriochlorophyll . Acid was produced from glycerol, erythrose, ribose, -xylose, galactose, glucose, fructose, mannitol, cellobiose, maltose, lactose, melibiose, turanose, -lyxose, -tagatose, -fucose, -arabitol and -arabitol after inoculating for 24 h and weakly positive results were also detected after 48 h in API 50CH strips with -arabinose, -arabinose, -xylose, adonitol, mannose, aesculin, salicin, sucrose, mycose and -fucose. The predominant fatty acids were Cω7 and/or Cω6, C, C and 11-methyl Cω7. The major polar lipids of ZH114 were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and two unidentified lipids. The major respiratory quinone was ubiquinone Q-10. The genomic DNA G+C content of strain ZH114 was 63.8 mol%. Based on this phenotypic, chemotaxonomic and phylogenetic analysis, strain ZH114 should be classified as a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is ZH114 ( = JCM 19383 = KCTC 32468).

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2015-04-01
2020-01-27
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