A novel actinomycete, designated strain A31T, was isolated from the surface of weathered biotite in Susong, Anhui Province, China. The organism grew optimally at 30 °C, at pH 8.0 and with 1 % (w/v) NaCl. Strain A31T had A3α as the cell-wall peptidoglycan type and galactose, mannose and rhamnose as whole-cell sugars. Anteiso-C15 : 0 and anteiso-C17 : 0 were the major cellular fatty acids and MK-9(H2) was the predominant respiratory quinone. In addition, the total polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylmonomethylethanolamine and four glycolipids. The genomic DNA G+C content of strain A31T was 70.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain A31T was related most closely to Sinomonas albida LC13T (98.3 % similarity), Sinomonas atrocyanea DSM 20127T (98.2 %), Sinomonas soli CW 59T (98.1 %), Sinomonas flava CW 108T (97.8 %), ‘Sinomonasmesophila’ MPKL 26 (97.3 %), Sinomonas echigonensis LC10T (97.1 %) and ‘Sinomonas notoginsengisoli’ SYP-B575 (96.7 %). DNA–DNA hybridization studies with the new isolate showed relatedness values of 16.0–56.6 % with its six closest neighbours. Based on phenotypic, chemotaxonomic and phylogenetic analysis, strain A31T represents a novel species of the genus Sinomonas, for which the name Sinomonassusongensis sp. nov. is proposed. The type strain is A31T ( = DSM 28245T = CCTCC AB 2014068T).
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Funding
This study was supported by the:
National Natural Science Foundation of China
(Award 41171257 and 41201251)
Fundamental Research Funds for the Central Universities
(Award KYZ201158)
CollinsM. D.(1985). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by
GoodfellowM.,
MinnikinD. E.
. London:: Academic Press;.
De LeyJ.,
CattoirH.,
ReynaertsA.(1970). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem12, 133–142. [View Article][PubMed]
DingL.,
HiroseT.,
YokotaA.(2009). Four novel Arthrobacter species isolated from filtration substrate. . Int J Syst Evol Microbiol59, 856–862. [View Article][PubMed]
GordonR. E.,
BarnettD. A.,
HanderhanJ. E.,
PangC. H.-N.(1974).Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol24, 54–63. [View Article]
KimuraM.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol16, 111–120. [View Article][PubMed]
MesbahM.,
PremachandranU.,
WhitmanW. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. . Int J Syst Bacteriol39, 159–167. [View Article]
MinnikinD. E.,
O’DonnellA. G.,
GoodfellowM.,
AldersonG.,
AthalyeM.,
SchaalA.,
ParlettJ. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods2, 233–241. [View Article]
PrabhuD. M.,
QuadriS. R.,
ChengJ.,
LiuL.,
ChenW.,
YangY.,
HozzeinW. N.,
LingappaK.,
LiW.-J.(2014).Sinomonas mesophila sp. nov., isolated from ancient fort soil. . J Antibiot (Tokyo) [Epub ahead of print]. [View Article][PubMed]
StackebrandtE.,
GoebelB. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol44, 846–849. [View Article]
TimkeM.,
Wang-LieuN. Q.,
AltendorfK.,
LipskiA.(2005). Community structure and diversity of biofilms from a beer bottling plant as revealed using 16S rRNA gene clone libraries. . Appl Environ Microbiol71, 6446–6452. [View Article][PubMed]
WayneL. G.,
BrennerD. J.,
ColwellR. R.,
GrimontP. A. D.,
KandlerO.,
KrichevskyM. I.,
MooreL. H.,
MooreW. E. C.,
MurrayR. G. E. et al.(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol37, 463–464. [View Article]
WieserM.,
DennerE. B. M.,
KämpferP.,
SchumannP.,
TindallB.,
SteinerU.,
VybiralD.,
LubitzW.,
MaszenanA. M. et al.(2002). Emended descriptions of the genus Micrococcus, Micrococcus luteus (Cohn 1872) and Micrococcus lylae (Kloos et al. 1974). . Int J Syst Evol Microbiol52, 629–637.[PubMed][CrossRef]
ZhouY.,
ChenX.,
ZhangY.,
WangW.,
XuJ.(2012). Description of Sinomonas soli sp. nov., reclassification of Arthrobacter echigonensis and Arthrobacter albidus (Ding et al. 2009) as Sinomonas echigonensis comb. nov. and Sinomonas albida comb. nov., respectively, and emended description of the genus Sinomonas
. . Int J Syst Evol Microbiol62, 764–769. [View Article][PubMed]