1887

Abstract

A novel type of mineral-weathering bacterium was isolated from the weathered surface of rock (mica schist) collected from Susong (Anhui, China). Cells of strain L226 were Gram-stain-negative. The strain grew optimally at 30 °C, with 1 % (w/v) NaCl and at pH 7.0 in trypticase soy broth. On the basis of 16S rRNA gene phylogeny, strain L226 was shown to belong to the genus and the closest phylogenetic relatives were WSM5005 (98.3 %), NBRC 101816 (98.2 %), STM678 (97.2 %) and JPY461 (97.1 %). The DNA G+C content was 63.5 mol% and the respiratory quinone was Q-8. The major fatty acids were C, C cyclo and C cyclo ω8. The polar lipid profile of strain L226 consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, unknown lipids and unidentified aminophospholipids. Based on the low level of DNA–DNA relatedness (ranging from 25.8 % to 34.4 %) to the tested type strains of species of the genus and unique phenotypic characteristics, it is suggested that strain L226 represents a novel species of the genus , for which the name sp. nov., is proposed. The type strain is L226 ( = CCTCC AB2014142 = JCM 30231).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000059
2015-03-01
2019-10-17
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/3/1031.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000059&mimeType=html&fmt=ahah

References

  1. Aizawa T. , Bao Ve N. , Vijarnsorn P. , Nakajima M. , Sunairi M. . ( 2010; ). Burkholderia acidipaludis sp. nov., aluminium-tolerant bacteria isolated from Chinese water chestnut (Eleocharis dulcis) growing in highly acidic swamps in South-East Asia. . Int J Syst Evol Microbiol 60:, 2036–2041. [CrossRef] [PubMed]
    [Google Scholar]
  2. Collins M. D. , Pirouz T. , Goodfellow M. , Minnikin D. E. . ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100:, 221–230. [CrossRef] [PubMed]
    [Google Scholar]
  3. De Ley J. , Cattoir H. , Reynaerts A. . ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef] [PubMed]
    [Google Scholar]
  4. De Meyer S. E. , Cnockaert M. , Ardley J. K. , Maker G. , Yates R. , Howieson J. G. , Vandamme P. . ( 2013; ). Burkholderia sprentiae sp. nov., isolated from Lebeckia ambigua root nodules. . Int J Syst Evol Microbiol 63:, 3950–3957. [CrossRef] [PubMed]
    [Google Scholar]
  5. De Meyer S. E. , Cnockaert M. , Ardley J. K. , Van Wyk B. E. , Vandamme P. A. , Howieson J. G. . ( 2014; ). Burkholderia dilworthii sp. nov., isolated from Lebeckia ambigua root nodules. . Int J Syst Evol Microbiol 64:, 1090–1095. [CrossRef] [PubMed]
    [Google Scholar]
  6. Dittmer J. C. , Lester R. L. . ( 1964; ). A simple, specific spray for the detection of phospholipids on thin-layer chromatograms. . J Lipid Res 5:, 126–127.[PubMed]
    [Google Scholar]
  7. Draghi W. O. , Peeters C. , Cnockaert M. , Snauwaert C. , Wall L. G. , Zorreguieta A. , Vandamme P. . ( 2014; ). Burkholderia cordobensis sp. nov., from agricultural soils. . Int J Syst Evol Microbiol 64:, 2003–2008. [CrossRef] [PubMed]
    [Google Scholar]
  8. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  9. Felsenstein J. . ( 1993; ). phylip (phylogeny inference package), version 3.5c. . Distributed by the author. Department of Genome Sciences, University of Washington:, Seattle, USA;.
  10. Garrity G. M. , Bell J. A. , Liburn T. . ( 2005; ). Family I. Burkholderiaceae fam. nov.. In Bergey’s Manual of Systematic Bacteriololgy , , 2nd edn., vol. 2, p. 575. Edited by Brenner D. J. , Krieg N. R. , Staley J. T. , Garrity G. M. . . New York:: Springer;. [CrossRef]
    [Google Scholar]
  11. Gillis M. , Van Van T. , Bardin R. , Goor M. , Hebbar P. , Willems A. , Segers P. , Kersters K. , Heulin T. , Fernandez M. P. . ( 1995; ). Polyphasic taxonomy in the genus Burkholderia leading to an emended description of the genus and proposition of Burkholderia vietnamiensis sp. nov. for N2-fixing isolates from rice in Vietnam. . Int J Syst Bacteriol 45:, 274–289. [CrossRef]
    [Google Scholar]
  12. Gordon R. E. , Barnett D. A. , Handerhan J. E. , Pang C. H.-N. . ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol 24:, 54–63. [CrossRef]
    [Google Scholar]
  13. Huang J. , Sheng X. F. , Xi J. , He L. Y. , Huang Z. , Wang Q. , Zhang Z. D. . ( 2014; ). Depth-related changes in community structure of culturable mineral weathering bacteria and in weathering patterns caused by them along two contrasting soil profiles. . Appl Environ Microbiol 80:, 29–42. [CrossRef] [PubMed]
    [Google Scholar]
  14. Jacin H. , Mishkin A. R. . ( 1965; ). Separation of carbohydrates on borate-impregnated silica gel G plates. . J Chromatogr A 18:, 170–173. [CrossRef] [PubMed]
    [Google Scholar]
  15. Kang S. R. , Srinivasan S. , Lee S. S. . ( 2014; ). Burkholderia eburnea sp. nov., isolated from peat soil. . Int J Syst Evol Microbiol 64:, 1108–1115. [CrossRef] [PubMed]
    [Google Scholar]
  16. Kimura M. . ( 1983; ). The Neutral Theory of Molecular Evolution. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  17. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. , Yi H. , Won S. , Chun J. . ( 2012; ). Introducing EzTaxon-e: a praokaryotic 16SrRNA gene sequence database with phylotypes that represent uncultured specise. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef]
    [Google Scholar]
  18. Lane D. J. . ( 1991; ). 16S/23S rRNA Sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E. , Goodfellow M. . . New York:: Wiley;.
    [Google Scholar]
  19. Lányi B. . ( 1988; ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19:, 1–67. [CrossRef]
    [Google Scholar]
  20. Liu X. Y. , Li C. X. , Luo X. J. , Lai Q. L. , Xu J. H. . ( 2014; ). Burkholderia jiangsuensis sp. nov., a methyl parathion degrading bacterium, isolated from methyl parathion contaminated soil. . Int J Syst Evol Microbiol 64:, 3247–3253. [CrossRef] [PubMed]
    [Google Scholar]
  21. Marmur J. . ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  22. Mavengere N. R. , Ellis A. G. , Le Roux J. J. . ( 2014; ). Burkholderia aspalathi sp. nov., isolated from root nodules of the South African legume Aspalathus abietina Thunb.. Int J Syst Evol Microbiol 64:, 1906–1912. [CrossRef] [PubMed]
    [Google Scholar]
  23. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. . Int J Syst Evol Microbiol 39:, 159–167.
    [Google Scholar]
  24. Minnikin D. E. , O’Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984; ). An integrated procedure for extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  25. Murray R. G. E. , Doetsch R. N. , Robinow F. . ( 1994; ). Determinative and cytological light microscopy. . In Methods for General and Molecular Bacteriology, pp. 21–41. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  26. Peeters C. , Zlosnik J. E. , Spilker T. , Hird T. J. , LiPuma J. J. , Vandamme P. . ( 2013; ). Burkholderia pseudomultivorans sp. nov., a novel Burkholderia cepacia complex species from human respiratory samples and the rhizosphere. . Syst Appl Microbiol 36:, 483–489. [CrossRef] [PubMed]
    [Google Scholar]
  27. Ross H. N. M. , Grant W. D. , Harris J. E. . ( 1985; ). Lipids in archaebacterial taxonomy. . In Chemical Methods in Bacterial Systematics, pp. 289–300. Edited by Goodfellow M. , Minnikin D. E. . . London:: Academic Press;.
    [Google Scholar]
  28. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  29. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
  30. Sheu S. Y. , Chou J. H. , Bontemps C. , Elliott G. N. , Gross E. , James E. K. , Sprent J. I. , Young J. P. W. , Chen W. M. . ( 2012; ). Burkholderia symbiotica sp. nov., isolated from root nodules of Mimosa spp. native to north-east Brazil. . Int J Syst Evol Microbiol 62:, 2272–2278. [CrossRef] [PubMed]
    [Google Scholar]
  31. Sheu S. Y. , Chou J. H. , Bontemps C. , Elliott G. N. , Gross E. , dos Reis Junior F. B. , Melkonian R. , Moulin L. , James E. K. et al. ( 2013; ). Burkholderia diazotrophica sp. nov., isolated from root nodules of Mimosa spp.. Int J Syst Evol Microbiol 63:, 435–441. [CrossRef] [PubMed]
    [Google Scholar]
  32. Spilker T. , Baldwin A. , Bumford A. , Dowson C. G. , Mahenthiralingam E. , LiPuma J. J. . ( 2009; ). Expanded multilocus sequence typing for burkholderia species. . J Clin Microbiol 47:, 2607–2610. [CrossRef] [PubMed]
    [Google Scholar]
  33. Suárez-Moreno Z. R. , Caballero-Mellado J. , Coutinho B. G. , Mendonça-Previato L. , James E. K. , Venturi V. . ( 2012; ). Common features of environmental and potentially beneficial plant-associated Burkholderia . . Microb Ecol 63:, 249–266. [CrossRef] [PubMed]
    [Google Scholar]
  34. Tamaoka J. , Katayama-Fujimura Y. , Kuraishi H. . ( 1983; ). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. . J Appl Bacteriol 54:, 31–36. [CrossRef]
    [Google Scholar]
  35. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  36. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  37. Valverde A. , Delvasto P. , Peix A. , Velázquez E. , Santa-Regina I. , Ballester A. , Rodríguez-Barrueco C. , García-Balboa C. , Igual J. M. . ( 2006; ). Burkholderia ferrariae sp. nov., isolated from an iron ore in Brazil. . Int J Syst Evol Microbiol 56:, 2421–2425. [CrossRef] [PubMed]
    [Google Scholar]
  38. Vandamme P. A. R. , Govan J. R. W. , Lipuma J. J. . ( 2007; ). Diversity and role of Burkholderia spp.. In Burkholderia Molecular Microbiology and Genomics, vol. 1, pp. 1–28. Edited by Coenye T. , Vandamme P. . . Wymondham:: Horizon Bioscience;.
    [Google Scholar]
  39. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. et al. ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  40. Yabuuchi E. , Kosako Y. , Oyaizu H. , Yano I. , Hotta H. , Hashimoto Y. , Ezaki T. , Arakawa M. . ( 1992; ). Proposal of Burkholderia gen. nov. and transfer of seven species of the genus Pseudomonas homology group II to the new genus, with the type species Burkholderia cepacia (Palleroni and Holmes 1981) comb. nov.. Microbiol Immunol 36:, 1251–1275. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000059
Loading
/content/journal/ijsem/10.1099/ijs.0.000059
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error