@article{mbs:/content/journal/ijsem/10.1099/ijs.0.000057, author = "Zhang, Lei and Pan, Yuanyuan and Wang, Kaibiao and Zhang, Xiaoxia and Zhang, Cheng and Zhang, Shuang and Fu, Xiaowei and Jiang, Juquan", title = "Pseudomonas zhaodongensis sp. nov., isolated from saline and alkaline soils", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2015", volume = "65", number = "Pt_3", pages = "1022-1030", doi = "https://doi.org/10.1099/ijs.0.000057", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.000057", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "Strain NEAU-ST5-21T was isolated from saline and alkaline soils in Zhaodong City, Heilongjiang Province, China. It was aerobic, Gram-stain-negative, rod-shaped and motile with a polar flagellum. It produced yellow–orange colonies with a smooth surface, and grew in the presence of 0–5 % (w/v) NaCl (optimum 0 %, w/v), at temperatures of 20–40 °C (optimum 28 °C) and at pH 7–11 (optimum pH 7). Phylogenetic analyses based on the separate 16S rRNA gene sequences and concatenated 16S rRNA, gyrB and rpoD gene sequences indicated that strain NEAU-ST5-21T belongs to the genus Pseudomonas in the class Gammaproteobacteria . The most closely related species is Pseudomonas xanthomarina , whose type strain (KMM 1447T) showed gene sequence similarities of 99.0 % for 16S rRNA, 81.8 % for gyrB and 85.0 % for rpoD with strain NEAU-ST5-21T. DNA–DNA hybridization values between strain NEAU-ST5-21T and P. xanthomarina DSM 18231T, Pseudomonas kunmingensis CGMCC 1.12273T, Pseudomonas stutzeri DSM 5190T, Pseudomonas oleovorans subsp. lubricantis DSM 21016T, Pseudomomas chengduensis CGMCC 2318T, Pseudomonas alcaliphila DSM 17744T and Pseudomonas toyotomiensis DSM 26169T were 52±0 % to 25±2 %. The DNA G+C content of strain NEAU-ST5-21T was 65 mol%. The major fatty acids (>10 %) were C18 : 1ω7c and/or C18 : 1ω6c, C16 : 1ω7c and/or C16 : 1ω6c and C16 : 0, the predominant respiratory quinone was ubiquinone 9, and polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, one unknown phospholipid, phosphatidylglycerol, one unknown aminolipid, one unknown lipid and a glycolipid. The proposed name is Pseudomonas zhaodongensis sp. nov., NEAU-ST5-21T ( = ACCC 06362T = DSM 27559T) being the type strain.", }