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Volume 65, Issue Pt_3

sp. nov., isolated from saline and alkaline soils Free

Abstract

Strain NEAU-ST5-21 was isolated from saline and alkaline soils in Zhaodong City, Heilongjiang Province, China. It was aerobic, Gram-stain-negative, rod-shaped and motile with a polar flagellum. It produced yellow–orange colonies with a smooth surface, and grew in the presence of 0–5 % (w/v) NaCl (optimum 0 %, w/v), at temperatures of 20–40 °C (optimum 28 °C) and at pH 7–11 (optimum pH 7). Phylogenetic analyses based on the separate 16S rRNA gene sequences and concatenated 16S rRNA, and gene sequences indicated that strain NEAU-ST5-21 belongs to the genus in the class . The most closely related species is , whose type strain (KMM 1447) showed gene sequence similarities of 99.0 % for 16S rRNA, 81.8 % for and 85.0 % for with strain NEAU-ST5-21. DNA–DNA hybridization values between strain NEAU-ST5-21 and DSM 18231, CGMCC 1.12273, DSM 5190, DSM 21016, CGMCC 2318, DSM 17744 and DSM 26169 were 52±0 % to 25±2 %. The DNA G+C content of strain NEAU-ST5-21 was 65 mol%. The major fatty acids (>10 %) were Cω7 and/or Cω6, Cω7 and/or Cω6 and C, the predominant respiratory quinone was ubiquinone 9, and polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, one unknown phospholipid, phosphatidylglycerol, one unknown aminolipid, one unknown lipid and a glycolipid. The proposed name is sp. nov., NEAU-ST5-21 ( = ACCC 06362 = DSM 27559) being the type strain.

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31370087)
  • Natural Science Foundation of Heilongjiang Province of China (Award C201417)
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2015-03-01
2024-04-20
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Pseudomonas zhaodongensis sp. nov., isolated from saline and alkaline soils
Int J Syst Evol Microbiol 65, 1022 (2015); https://doi.org/10.1099/ijs.0.000057
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