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Abstract

A Gram-staining-negative, non-motile, yellow-coloured, rod-shaped bacterium, designated S44, was isolated from bankside soil of Xixi wetland, located in Zhejiang province, China. Growth of strain S44 was observed at 6–37 °C (optimum, 28 °C) and at pH 6.0–9.0 (optimum, 7.0). No growth occurred in the presence of >2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain S44 represented a member of the genus , showing the highest sequence similarities to the sequences from DSM 4002 (96.9 %), WB 3.2-61 (96.6 %) and NCBI 105008 (96.5 %). The G+C content of the genomic DNA was 33.6 mol%. The predominant cellular fatty acids were C, iso-C, anteiso-C and summed feature 3 (comprising Cω7 and/or iso-C 2-OH), and the major respiratory quinone was menaquinone 6 (MK-6). The major polar lipids were phosphatidylethanolamine, two unknown aminolipids, two unknown aminophospholipids and four unknown polar lipids. On the basis of the phenotypic and genotypic data, it is proposed that the isolate S44 be classified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S44 ( = CGMCC 1.12811 = NBRC 110389).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 21137003 and 21077090)
  • 973 Program (Award 2014CB441103)
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2015-03-01
2024-03-29
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