1887

Abstract

A psychrotolerant, Gram-stain-negative, motile, aerobic, peritrichous bacterium, strain DJC1-1, was isolated from Lake Dajiaco, Tibetan Plateau, China. The strain was negative for citrate utilization, lipase activity and α-glucosidase, but positive for the Voges–Proskauer reaction and -acetyl-β-glucosaminidase. 16S rRNA gene sequence analysis indicated that ATCC 29927, ATCC 13337, DSM 30063 and DSM 4540 were the closest relatives of strain DJC1-1, with similarities of 97.76, 96.80, 97.71 and 97.58 %, respectively. The DNA G+C content of strain DJC1-1 was 53.9 mol%. The predominant fatty acids were C and C cyclo. Based on these characteristics, strain DJC1-1 can be assigned to the genus . In DNA–DNA hybridization tests, strain DJC1-1 shared 50.6, 35.1, 36.5 and 18.1 % DNA–DNA relatedness with the type strains of , , and , respectively. The growth temperature ranged from 0 to 40 °C, with optimum growth at 15 °C. Physiological and biochemical tests differentiated strain DJC1-1 from the type strains of recognized species of the genus . Therefore, strain DJC1-1 is identified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DJC1-1 ( = JCM 30077 = CGMCC1.12806).

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2015-03-01
2020-01-29
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References

  1. Brenner D. J.. ( 1978;). Characterization and clinical identification of Enterobacteriaceae by DNA hybridization. . Prog Clin Pathol 7:, 71–117.[PubMed]
    [Google Scholar]
  2. Breznak J. A., Costilow R. N.. ( 1994;). Physicochemical factors in growth. . In Methods for General and Molecular Bacteriology, pp. 137–154. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  3. De Ley J., Cattoir H., Reynaerts A.. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef][PubMed]
    [Google Scholar]
  4. Embley T. M.. ( 1991;). The linear PCR reaction: a simple and robust method for sequencing amplified rRNA genes. . Lett Appl Microbiol 13:, 171–174. [CrossRef][PubMed]
    [Google Scholar]
  5. Gevers D., Huys G., Swings J.. ( 2001;). Applicability of rep-PCR fingerprinting for identification of Lactobacillus species. . FEMS Microbiol Lett 205:, 31–36. [CrossRef][PubMed]
    [Google Scholar]
  6. Greipsson S., Priest F. G.. ( 1983;). Numerical taxonomy of Hafnia alvei. . Int J Syst Bacteriol 33:, 470–475. [CrossRef]
    [Google Scholar]
  7. Huss V. A., Festl H., Schleifer K. H.. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192. [CrossRef][PubMed]
    [Google Scholar]
  8. Huys G., Cnockaert M., Abbott S. L., Janda J. M., Vandamme P.. ( 2010;). Hafnia paralvei sp. nov., formerly known as Hafnia alvei hybridization group 2. . Int J Syst Evol Microbiol 60:, 1725–1728. [CrossRef][PubMed]
    [Google Scholar]
  9. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  10. Kim M., Oh H.-S., Park S.-C., Chun J.. ( 2014;). Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. . Int J Syst Evol Microbiol 64:, 346–351. [CrossRef][PubMed]
    [Google Scholar]
  11. Marmur J.. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  12. Masco L., Huys G., Gevers D., Verbrugghen L., Swings J.. ( 2003;). Identification of Bifidobacterium species using rep-PCR fingerprinting. . Syst Appl Microbiol 26:, 557–563. [CrossRef][PubMed]
    [Google Scholar]
  13. McBee M., Schauer D.. ( 2006;). The genus Hafnia. . In The Prokaryotes, pp. 215–218. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E... New York:: Springer;. [CrossRef]
    [Google Scholar]
  14. Møller V.. ( 1954;). Distribution of amino acid decarboxylases in Enterobacteriaceae. . Acta Pathol Microbiol Scand 35:, 259–277. [CrossRef][PubMed]
    [Google Scholar]
  15. Reasoner D. J., Geldreich E. E.. ( 1985;). A new medium for the enumeration and subculture of bacteria from potable water. . Appl Environ Microbiol 49:, 1–7.[PubMed]
    [Google Scholar]
  16. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  17. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
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