1887

Abstract

A psychrotolerant, Gram-stain-negative, motile, aerobic, peritrichous bacterium, strain DJC1-1, was isolated from Lake Dajiaco, Tibetan Plateau, China. The strain was negative for citrate utilization, lipase activity and α-glucosidase, but positive for the Voges–Proskauer reaction and -acetyl-β-glucosaminidase. 16S rRNA gene sequence analysis indicated that ATCC 29927, ATCC 13337, DSM 30063 and DSM 4540 were the closest relatives of strain DJC1-1, with similarities of 97.76, 96.80, 97.71 and 97.58 %, respectively. The DNA G+C content of strain DJC1-1 was 53.9 mol%. The predominant fatty acids were C and C cyclo. Based on these characteristics, strain DJC1-1 can be assigned to the genus . In DNA–DNA hybridization tests, strain DJC1-1 shared 50.6, 35.1, 36.5 and 18.1 % DNA–DNA relatedness with the type strains of , , and , respectively. The growth temperature ranged from 0 to 40 °C, with optimum growth at 15 °C. Physiological and biochemical tests differentiated strain DJC1-1 from the type strains of recognized species of the genus . Therefore, strain DJC1-1 is identified as representing a novel species of the genus for which the name sp. nov. is proposed. The type strain is DJC1-1 ( = JCM 30077 = CGMCC1.12806).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31100369, 41201059, 41171050 and 41371084)
  • National Basic Research Program of China (Award 2012CB956100)
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2015-03-01
2021-10-23
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