1887

Abstract

An obligately anaerobic bacterial strain designated KC3 was isolated from a rice straw-degrading culture, for which soil of a Japanese rice field was used as the inoculum. Cells of strain KC3 were determined to be non-cellulolytic, Gram-stain-positive, non-motile, ellipsoidal, spore-forming rods, 0.8–1×4–25 µm. Endospores were formed at a terminal position in elongated cells (12–25 µm, mean 15 µm). The temperature range for growth was 20–50 °C, with an optimum at 37 °C. The pH range for growth was 5.0–7.5, with an optimum at pH 6.0 (slightly acidophilic). Strain KC3 fermented cellobiose to lactate, butyrate, acetate, formate, hydrogen and carbon dioxide. The major cellular fatty acids (>10 %) were C, C and C cyclo 11,12 dimethylacetal. The DNA G+C content of strain KC3 was 37.5 mol%. 16S rRNA gene sequence analysis revealed that strain KC3 shared low sequence similarity (<93 %) with type strains of the genus ( rRNA cluster I). Analyses of the DNA gyrase A and ATP synthase beta subunit sequences supported the affiliation of strain KC3 to the genus The evidence presented here indicates that strain KC3 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain of is KC3 ( = DSM 28571 = NBRC 110163).

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2015-03-01
2019-10-19
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