A Gram-stain-negative, facultatively anaerobic, oxidase- and catalase-positive, rod-shaped bacterium, strain SYM1T, was isolated from a culture of Symbiodinium sp., an algal symbiont of the sea anemone Aiptasia tagetes collected in Puerto Rico. Growth was observed at 4–40 °C (optimum 30 °C), at pH 5.0–11.0 (optimum pH 8.0) and with 0.5–8 % (optimum 2 %) (w/v) NaCl. Phylogenetic analyses of 16S rRNA gene sequences showed that strain SYM1T was a member of the genus Neptunomonaswith the type strain of Neptunomonas naphthovorans as the closest phylogenetic relative with a pairwise sequence similarity of 98.15 %. However, DNA–DNA relatedness between strain SYM1T and N. naphthovorans CIP 106451T was 24 %. Moreover, strain SYM1T could be distinguished from its closest relative by several phenotypic characteristics such as NaCl, pH and temperature tolerance, nitrate reduction and utilization of carbon substrates. The major cellular fatty acids were C16 : 0, C18 : 1ω7c and summed feature 3 (comprising C16 : 1ω7c and/or iso-C15 : 0 2-OH). The genomic DNA G+C content of strain SYM1T was 45 mol%. Ubiquinone-8 (Q-8) was the only respiratory quinone detected. Based on a polyphasic taxonomic characterization, strain SYM1T represents a novel species of the genus Neptunomonas, for which the name Neptunomonas phycophila sp. nov. is proposed. The type strain is SYM1T ( = LMG 28329T = CECT 8716T).
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Funding
This study was supported by the:
FEDER
Portuguese Foundation for Science and Technology (FCT)
(Award PTDC/AAC-AMB/118873/2010 – FCOMP-01-0124-FEDER-019328 and PTDC/MAR/113962/2009)
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