An aerobic, Gram-stain-negative, rod-shaped and polar-flagellated bacterium, designated strain CC-MHH0089T, was isolated from a soil sample taken on Matsu Island (Taiwan). Strain CC-MHH0089T grew at 15–30 °C and pH 5.0–10.0 and tolerated ≤8 % (w/v) NaCl. 16S rRNA gene sequence analysis showed high pairwise sequence similarity to Pseudomonas azotifigens 6H33bT (97.3 %) and Pseudomonas balearica SP1402T (96.7 %) and lower sequence similarity to other strains (<96.0 %). In DNA–DNA reassociation experiments, the relatedness of strain CC-MHH0089T to P. azotifigens JCM 12708T was 38.3 % (reciprocal value 19.5 %). Evolutionary trees reconstructed on the basis of 16S rRNA, gyrB and rpoB gene sequences revealed a varying phylogenetic neighbourhood of strain CC-MHH0089T with regard to the most closely related type strains. The predominant quinone system was ubiquinone 9 (Q-9) and the DNA G+C content was 63.6 mol%. The major fatty acids were C12 : 0, C16 : 0, C17 : 0, C19 : 0 cyclo ω8c and summed features 2 (C14 : 0 3-OH/iso-C16 : 1 I), 3 (C16 : 1ω7c/C16 : 1ω6c) and 8 (C18 : 1ω7c/C18 : 1ω6c). The major polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol. According to its distinct phylogenetic, phenotypic and chemotaxonomic features, strain CC-MHH0089T is proposed to represent a novel species within the genus Pseudomonas, for which the name Pseudomonas matsuisoli sp. nov. is proposed. The type strain is CC-MHH0089T ( = BCRC 80771T = JCM 30078T).
Ait TayebL.,
AgeronE.,
GrimontF.,
GrimontP. A. D.(2005). Molecular phylogeny of the genus Pseudomonas based on rpoB sequences and application for the identification of isolates. . Res Microbiol156, 763–773. [View Article][PubMed]
AnzaiY.,
KimH.,
ParkJ.-Y.,
WakabayashiH.,
OyaizuH.(2000). Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. . Int J Syst Evol Microbiol50, 1563–1589. [View Article][PubMed]
CollinsM. D.(1985). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by
GoodfellowM.,
MinnikinD. E.
. London:: Academic Press;.
HeinerC. R.,
HunkapillerK. L.,
ChenS. M.,
GlassJ. I.,
ChenE. Y.(1998). Sequencing multimegabase-template DNA with BigDye terminator chemistry. . Genome Res8, 557–561.[PubMed]
KennedyC.,
RudnickP.(2005). Genus II. Azomonas Winogradsky 1938, 391AL
. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 2B, pp. 379–384. Edited by
BrennerD. J.,
KriegN. R.,
StaleyJ. T.,
GarrityG. M.
. New York:: Springer;.
KennedyC.,
RudnickP.,
MacDonaldM. L.,
MeltonT.(2005). Genus III. Azotobacter Beijerinck 1901, 567AL
. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 2B, pp. 384–402. Edited by
BrennerD. J.,
KriegN. R.,
StaleyJ. T.,
GarrityG. M.
. New York:: Springer;.
Meier-KolthoffJ. P.,
GökerM.,
SpröerC.,
KlenkH.-P.(2013). When should a DDH experiment be mandatory in microbial taxonomy?. Arch Microbiol195, 413–418. [View Article][PubMed]
MesbahM.,
PremachandranU.,
WhitmanW. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol39, 159–167. [View Article]
MinnikinD. E.,
O’DonnellA. G.,
GoodfellowM.,
AldersonG.,
AthalyeM.,
SchaalK.,
ParlettJ. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods2, 233–241. [View Article]
MurrayR. G. E.,
DoetschR. N.,
RobinowC. F.(1994). Determination and cytological light microscopy. . In Methods for General and Molecular Bacteriology, pp. 32–34. Edited by
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
. Washington, DC:: American Society for Microbiology;.
OyaizuH.,
KomagataK.(1983). Grouping of Pseudomonas species on the basis of cellular fatty acid composition and the quinone system with special reference to the existence of 3-hydroxy fatty acids. . J Gen Appl Microbiol29, 17–40. [View Article]
PalleroniN. J.(1984). Genus I. Pseudomonas Migula 1894. . In Bergey’s Manual of Systematic Bacteriology, vol. 1, pp. 141–199. Edited by
KriegN. R.,
HoltJ. G.
. Baltimore:: Williams & Wilkins;.
PalleroniN. J.(1994).Pseudomonas classification. A new case history in the taxonomy of gram-negative bacteria. . Antonie van Leeuwenhoek64, 231–251. [View Article][PubMed]
PeixA.,
Ramírez-BahenaM. H.,
VelázquezE.(2009). Historical evolution and current status of the taxonomy of genus Pseudomonas
. . Infect Genet Evol9, 1132–1147. [View Article][PubMed]
SneathP. H. A.,
StevensM.,
SackinM. J.(1981). Numerical taxonomy of Pseudomonas based on published records of substrate utilization. . Antonie van Leeuwenhoek47, 423–448. [View Article][PubMed]
WangQ.,
GarrityG. M.,
TiedjeJ. M.,
ColeJ. R.(2007). Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. . Appl Environ Microbiol73, 5261–5267. [View Article][PubMed]
WayneL. G.,
BrennerD. J.,
ColwellR. R.,
GrimontP. A. D.,
KandlerO.,
KrichevskyM. I.,
MooreL. H.,
MooreW. E. C.,
MurrayR. G. E.& other authors (1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol37, 463–464. [View Article]
YamamotoS.,
HarayamaS.(1995). PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains. . Appl Environ Microbiol61, 1104–1109.[PubMed]