1887

Abstract

An aerobic, Gram-stain-negative, rod-shaped and polar-flagellated bacterium, designated strain CC-MHH0089, was isolated from a soil sample taken on Matsu Island (Taiwan). Strain CC-MHH0089 grew at 15–30 °C and pH 5.0–10.0 and tolerated ≤8 % (w/v) NaCl. 16S rRNA gene sequence analysis showed high pairwise sequence similarity to 6H33b (97.3 %) and SP1402 (96.7 %) and lower sequence similarity to other strains (<96.0 %). In DNA–DNA reassociation experiments, the relatedness of strain CC-MHH0089 to JCM 12708 was 38.3 % (reciprocal value 19.5 %). Evolutionary trees reconstructed on the basis of 16S rRNA, and gene sequences revealed a varying phylogenetic neighbourhood of strain CC-MHH0089 with regard to the most closely related type strains. The predominant quinone system was ubiquinone 9 (Q-9) and the DNA G+C content was 63.6 mol%. The major fatty acids were C, C, C, C cyclo ω8 and summed features 2 (C 3-OH/iso-C I), 3 (Cω7/Cω6) and 8 (Cω7/Cω6). The major polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol. According to its distinct phylogenetic, phenotypic and chemotaxonomic features, strain CC-MHH0089 is proposed to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is CC-MHH0089 ( = BCRC 80771 = JCM 30078).

Funding
This study was supported by the:
  • Ministry of Science and Technology
  • Council of Agriculture, Executive Yuan
  • Ministry of Education, Taiwan under the ATU plan
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2015-03-01
2021-04-10
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