1887

Abstract

A novel actinomycete strain RY35-68, isolated from a peat swamp forest soil sample in Rayong Province, Thailand, was characterized using a polyphasic approach. The strain belonged to the genus based on morphological and chemotaxonomic characteristics. Cell-wall analysis revealed the presence of -diaminopimelic acid and -acetylmuramic acid in the peptidoglycan layer. The diagnostic sugar in whole-cell hydrolysates was identified as madurose. The predominant menaquinones were MK-9(H), MK-9(H) and MK-9(H). The major cellular fatty acids were C and iso-C The major polar lipids were diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside. The genomic DNA G+C content was 73.7 mol%. On the basis of 16S rRNA gene sequence similarity analysis, strain RY35-68 was closely related to the species JCM 6250 (97.5 %). The value of DNA–DNA relatedness between strain RY35-68 and JCM 6250 was 37.6–42.6 %. On the basis of its phenotypic characteristics and these results mentioned, this strain could be distinguished from the closely related type strain and represents a novel species of the genus , for which the name sp. nov. (type strain RY35-68 = JCM 19830 = TISTR 2211 = PCU 332) is proposed.

Funding
This study was supported by the:
  • Ratchadaphiseksomphot Endowment Fund
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2015-03-01
2024-03-28
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