1887

Abstract

Strain BUT-8, a Gram-stain-negative, non-motile and rod-shaped aerobic bacterium, was isolated from the activated sludge of a herbicide-manufacturing wastewater treatment facility. Comparative 16S rRNA gene sequence analysis revealed that strain BUT-8 clustered with species of the genus and was closely related to DSM 22393 (98.3 %) and KACC 11406 (98.7 %). The DNA G+C content of the genomic DNA was 70.6 mol%. The major respiratory quinone was ubiquinone-8, and the major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an aminolipid. The major cellular fatty acids were iso-C, iso-C, iso-C, iso-C, iso-C 3OH and summed feature 9 (comprising iso-Cω9 and/or C10-methyl). The DNA–DNA relatedness between strain BUT-8 and its closest phylogenetic neighbours was below 70 %. Phylogenetic, chemotaxonomic and phenotypic results clearly demonstrated that strain BUT-8 belongs to the genus and represents a novel species for which the name sp. nov. is proposed. The type strain is BUT-8 ( = CCTCC AB 2013087 = KACC 17141).

Funding
This study was supported by the:
  • National Science and Technology support plan (Award 2013AA102804)
  • National Natural Science Foundation of China (Award 31270157)
  • Program for New Century Excellent Talents in University (Award NCET-13-0861)
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2015-03-01
2024-03-28
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