1887

Abstract

Strain BUT-8, a Gram-stain-negative, non-motile and rod-shaped aerobic bacterium, was isolated from the activated sludge of a herbicide-manufacturing wastewater treatment facility. Comparative 16S rRNA gene sequence analysis revealed that strain BUT-8 clustered with species of the genus and was closely related to DSM 22393 (98.3 %) and KACC 11406 (98.7 %). The DNA G+C content of the genomic DNA was 70.6 mol%. The major respiratory quinone was ubiquinone-8, and the major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an aminolipid. The major cellular fatty acids were iso-C, iso-C, iso-C, iso-C, iso-C 3OH and summed feature 9 (comprising iso-Cω9 and/or C10-methyl). The DNA–DNA relatedness between strain BUT-8 and its closest phylogenetic neighbours was below 70 %. Phylogenetic, chemotaxonomic and phenotypic results clearly demonstrated that strain BUT-8 belongs to the genus and represents a novel species for which the name sp. nov. is proposed. The type strain is BUT-8 ( = CCTCC AB 2013087 = KACC 17141).

Funding
This study was supported by the:
  • National Science and Technology support plan (Award 2013AA102804)
  • National Natural Science Foundation of China (Award 31270157)
  • Program for New Century Excellent Talents in University (Award NCET-13-0861)
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2015-03-01
2021-08-01
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References

  1. Aslam Z., Yasir M., Jeon C.-O., Chung Y.-R. ( 2009 ). Lysobacter oryzae sp. nov., isolated from the rhizosphere of rice (Oryza sativa L.). . Int J Syst Evol Microbiol 59, 675680. [View Article] [PubMed]
    [Google Scholar]
  2. Bae H.-S., Im W.-T., Lee S.-T. ( 2005 ). Lysobacter concretionis sp. nov., isolated from anaerobic granules in an upflow anaerobic sludge blanket reactor. . Int J Syst Evol Microbiol 55, 11551161. [View Article] [PubMed]
    [Google Scholar]
  3. Bernardet J.-F., Nakagawa Y.,, Holmes B. & Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002 ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52, 10491070. [View Article] [PubMed]
    [Google Scholar]
  4. Bligh E.-G., Dyer W.-J. ( 1959 ). A rapid method of total lipid extraction and purification. . Can J Biochem Physiol 37, 911917. [View Article] [PubMed]
    [Google Scholar]
  5. Buck J.-D. ( 1982 ). Nonstaining (KOH) method for determination of gram reactions of marine bacteria. . Appl Environ Microbiol 44, 992993.[PubMed]
    [Google Scholar]
  6. Christensen P., Cook F. D. ( 1978 ). Lysobacter, a new genus of nonfruiting, gliding bacteria with a high base ratio. . Int J Syst Bacteriol 28, 367393. [View Article]
    [Google Scholar]
  7. Collins M.-D., Pirouz T., Goodfellow M., Minnikin D.-E. ( 1977 ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100, 221230. [View Article] [PubMed]
    [Google Scholar]
  8. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224229. [View Article]
    [Google Scholar]
  9. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  10. Fukuda W., Kimura T., Araki S., Miyoshi Y., Atomi H., Imanaka T. ( 2013 ). Lysobacter oligotrophicus sp. nov., isolated from an Antarctic freshwater lake in Antarctica. . Int J Syst Evol Microbiol 63, 33133318. [View Article] [PubMed]
    [Google Scholar]
  11. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y.-S., Lee J.-H., Yi H., Won S., Chun J. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [CrossRef]
    [Google Scholar]
  12. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [View Article] [PubMed]
    [Google Scholar]
  13. Kumar S., Tamura K., Nei M. ( 2004 ). mega3: Integrated software for Molecular evolutionary genetics analysis and sequence alignment. . Brief Bioinform 5, 150163. [View Article] [PubMed]
    [Google Scholar]
  14. Luo G., Shi Z., Wang G. ( 2012 ). Lysobacter arseniciresistens sp. nov., an arsenite-resistant bacterium isolated from iron-mined soil. . Int J Syst Evol Microbiol 62, 16591665. [View Article] [PubMed]
    [Google Scholar]
  15. Mandel M., Marmur J. ( 1968 ). Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. . Methods Enzymol 12, 195206. [View Article]
    [Google Scholar]
  16. Park J.-H., Kim R., Aslam Z., Jeon C.-O., Chung Y.-R. ( 2008 ). Lysobacter capsici sp. nov., with antimicrobial activity, isolated from the rhizosphere of pepper, and emended description of the genus Lysobacter . . Int J Syst Evol Microbiol 58, 387392. [View Article] [PubMed]
    [Google Scholar]
  17. Romanenko L.-A., Uchino M., Tanaka N., Frolova G.-M., Mikhailov V.-V. ( 2008 ). Lysobacter spongiicola sp. nov., isolated from a deep-sea sponge. . Int J Syst Evol Microbiol 58, 370374. [View Article] [PubMed]
    [Google Scholar]
  18. Sambrook J., Russell D. ( 2001 ). Molecular Cloning: a Laboratory Manual, , 3rd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;.
    [Google Scholar]
  19. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids . , MIDI Technical Note 101. Newark, DE: MIDI Inc.
    [Google Scholar]
  20. Smibert R., Krieg N.-R., Gerhardt P., Murray R., Wood W.-A., Krieg N.-R. ( 1994 ). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  21. Srinivasan S., Kim M.-K., Sathiyaraj G., Kim H.-B., Kim Y.-J., Yang D.-C. ( 2010 ). Lysobacter soli sp. nov., isolated from soil of a ginseng field. . Int J Syst Evol Microbiol 60, 15431547. [View Article] [PubMed]
    [Google Scholar]
  22. Ten L.-N., Jung H.-M., Im W.-T., Yoo S.-A., Lee S.-T. ( 2008 ). Lysobacter daecheongensis sp. nov., isolated from sediment of stream near the Daechung dam in South Korea. . J Microbiol 46(5), 519524. [CrossRef]
    [Google Scholar]
  23. Ten L.-N., Jung H.-M., Im W.-T., Yoo S.-A., Oh H.-M., Lee S.-T. ( 2009 ). Lysobacter panaciterrae sp. nov., isolated from soil of a ginseng field. . Int J Syst Evol Microbiol 59, 958963. [CrossRef]
    [Google Scholar]
  24. Thompson J.-D., Gibson T.-J., Plewniak F., Jeanmougin F., Higgins D.-G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [View Article] [PubMed]
    [Google Scholar]
  25. Tindall B. J., Sikorski J., Smibert R. M., Kreig N. R. ( 2007 ). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology, , 3rd edn., pp. 330393. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M. . Washington, DC, USA:: L. R. Snyder American Society for Microbiology;. [View Article]
    [Google Scholar]
  26. Wang Y., Dai J., Zhang L., Luo X., Li Y., Chen G., Tang Y., Meng Y., Fang C. ( 2009 ). Lysobacter ximonensis sp. nov., isolated from soil. . Int J Syst Evol Microbiol 59, 786789. [View Article] [PubMed]
    [Google Scholar]
  27. Wang G.-L., Wang L., Chen H.-H., Shen B., Li S.-P., Jiang J.-D. ( 2011 ). Lysobacter ruishenii sp. nov., a chlorothalonil-degrading bacterium isolated from a long-term chlorothalonil-contaminated soil. . Int J Syst Evol Microbiol 61, 674679. [View Article] [PubMed]
    [Google Scholar]
  28. Wayne L., Brenner D., Colwell R., Grimont P., Kandler O., Krichevsky M., Moore L., Moore W., Murray R. et al. ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
  29. Weon H.-Y., Kim B.-Y., Baek Y.-K., Yoo S.-H., Kwon S.-W., Stackebrandt E., Go S.-J. ( 2006 ). Two novel species, Lysobacter daejeonensis sp. nov. and Lysobacter yangpyeongensis sp. nov., isolated from Korean greenhouse soils. . Int J Syst Evol Microbiol 56, 947951. [View Article] [PubMed]
    [Google Scholar]
  30. Weon H.-Y., Kim B.-Y., Kim M.-K., Yoo S.-H., Kwon S.-W., Go S.-J., Stackebrandt E. ( 2007 ). Lysobacter niabensis sp. nov. and Lysobacter niastensis sp. nov., isolated from greenhouse soils in Korea. . Int J Syst Evol Microbiol 57, 548551. [View Article] [PubMed]
    [Google Scholar]
  31. Yassin A.-F., Chen W.-M., Hupfer H., Siering C., Kroppenstedt R.-M., Arun A.-B., Lai W.-A., Shen F.-T., Rekha P.-D., Young C.-C. ( 2007 ). Lysobacter defluvii sp. nov., isolated from municipal solid waste. . Int J Syst Evol Microbiol 57, 11311136. [View Article] [PubMed]
    [Google Scholar]
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