1887

Abstract

Strain BUT-8, a Gram-stain-negative, non-motile and rod-shaped aerobic bacterium, was isolated from the activated sludge of a herbicide-manufacturing wastewater treatment facility. Comparative 16S rRNA gene sequence analysis revealed that strain BUT-8 clustered with species of the genus and was closely related to DSM 22393 (98.3 %) and KACC 11406 (98.7 %). The DNA G+C content of the genomic DNA was 70.6 mol%. The major respiratory quinone was ubiquinone-8, and the major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an aminolipid. The major cellular fatty acids were iso-C, iso-C, iso-C, iso-C, iso-C 3OH and summed feature 9 (comprising iso-Cω9 and/or C10-methyl). The DNA–DNA relatedness between strain BUT-8 and its closest phylogenetic neighbours was below 70 %. Phylogenetic, chemotaxonomic and phenotypic results clearly demonstrated that strain BUT-8 belongs to the genus and represents a novel species for which the name sp. nov. is proposed. The type strain is BUT-8 ( = CCTCC AB 2013087 = KACC 17141).

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2015-03-01
2019-10-14
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References

  1. Aslam Z. , Yasir M. , Jeon C.-O. , Chung Y.-R. . ( 2009; ). Lysobacter oryzae sp. nov., isolated from the rhizosphere of rice (Oryza sativa L.). . Int J Syst Evol Microbiol 59:, 675–680. [CrossRef] [PubMed]
    [Google Scholar]
  2. Bae H.-S. , Im W.-T. , Lee S.-T. . ( 2005; ). Lysobacter concretionis sp. nov., isolated from anaerobic granules in an upflow anaerobic sludge blanket reactor. . Int J Syst Evol Microbiol 55:, 1155–1161. [CrossRef] [PubMed]
    [Google Scholar]
  3. Bernardet J.-F. , Nakagawa Y., , Holmes B. . & Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002; ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070. [CrossRef] [PubMed]
    [Google Scholar]
  4. Bligh E.-G. , Dyer W.-J. . ( 1959; ). A rapid method of total lipid extraction and purification. . Can J Biochem Physiol 37:, 911–917. [CrossRef] [PubMed]
    [Google Scholar]
  5. Buck J.-D. . ( 1982; ). Nonstaining (KOH) method for determination of gram reactions of marine bacteria. . Appl Environ Microbiol 44:, 992–993.[PubMed]
    [Google Scholar]
  6. Christensen P. , Cook F. D. . ( 1978; ). Lysobacter, a new genus of nonfruiting, gliding bacteria with a high base ratio. . Int J Syst Bacteriol 28:, 367–393. [CrossRef]
    [Google Scholar]
  7. Collins M.-D. , Pirouz T. , Goodfellow M. , Minnikin D.-E. . ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100:, 221–230. [CrossRef] [PubMed]
    [Google Scholar]
  8. Ezaki T. , Hashimoto Y. , Yabuuchi E. . ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  9. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  10. Fukuda W. , Kimura T. , Araki S. , Miyoshi Y. , Atomi H. , Imanaka T. . ( 2013; ). Lysobacter oligotrophicus sp. nov., isolated from an Antarctic freshwater lake in Antarctica. . Int J Syst Evol Microbiol 63:, 3313–3318. [CrossRef] [PubMed]
    [Google Scholar]
  11. Kim O.-S. , Cho Y.-J. , Lee K. , Yoon S.-H. , Kim M. , Na H. , Park S.-C. , Jeon Y.-S. , Lee J.-H. , Yi H. , Won S. , Chun J. . ( 2012; ). Introducing EzTaxon-e: a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721.[CrossRef]
    [Google Scholar]
  12. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  13. Kumar S. , Tamura K. , Nei M. . ( 2004; ). mega3: Integrated software for Molecular evolutionary genetics analysis and sequence alignment. . Brief Bioinform 5:, 150–163. [CrossRef] [PubMed]
    [Google Scholar]
  14. Luo G. , Shi Z. , Wang G. . ( 2012; ). Lysobacter arseniciresistens sp. nov., an arsenite-resistant bacterium isolated from iron-mined soil. . Int J Syst Evol Microbiol 62:, 1659–1665. [CrossRef] [PubMed]
    [Google Scholar]
  15. Mandel M. , Marmur J. . ( 1968; ). Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. . Methods Enzymol 12:, 195–206. [CrossRef]
    [Google Scholar]
  16. Park J.-H. , Kim R. , Aslam Z. , Jeon C.-O. , Chung Y.-R. . ( 2008; ). Lysobacter capsici sp. nov., with antimicrobial activity, isolated from the rhizosphere of pepper, and emended description of the genus Lysobacter . . Int J Syst Evol Microbiol 58:, 387–392. [CrossRef] [PubMed]
    [Google Scholar]
  17. Romanenko L.-A. , Uchino M. , Tanaka N. , Frolova G.-M. , Mikhailov V.-V. . ( 2008; ). Lysobacter spongiicola sp. nov., isolated from a deep-sea sponge. . Int J Syst Evol Microbiol 58:, 370–374. [CrossRef] [PubMed]
    [Google Scholar]
  18. Sambrook J. , Russell D. . ( 2001; ). Molecular Cloning: a Laboratory Manual, , 3rd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;.
    [Google Scholar]
  19. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids . , MIDI Technical Note 101. Newark, DE: MIDI Inc.
    [Google Scholar]
  20. Smibert R. , Krieg N.-R. , Gerhardt P. , Murray R. , Wood W.-A. , Krieg N.-R. . ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  21. Srinivasan S. , Kim M.-K. , Sathiyaraj G. , Kim H.-B. , Kim Y.-J. , Yang D.-C. . ( 2010; ). Lysobacter soli sp. nov., isolated from soil of a ginseng field. . Int J Syst Evol Microbiol 60:, 1543–1547. [CrossRef] [PubMed]
    [Google Scholar]
  22. Ten L.-N. , Jung H.-M. , Im W.-T. , Yoo S.-A. , Lee S.-T. . ( 2008; ). Lysobacter daecheongensis sp. nov., isolated from sediment of stream near the Daechung dam in South Korea. . J Microbiol 46(5):, 519–524.[CrossRef]
    [Google Scholar]
  23. Ten L.-N. , Jung H.-M. , Im W.-T. , Yoo S.-A. , Oh H.-M. , Lee S.-T. . ( 2009; ). Lysobacter panaciterrae sp. nov., isolated from soil of a ginseng field. . Int J Syst Evol Microbiol 59:, 958–963.[CrossRef]
    [Google Scholar]
  24. Thompson J.-D. , Gibson T.-J. , Plewniak F. , Jeanmougin F. , Higgins D.-G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  25. Tindall B. J. , Sikorski J. , Smibert R. M. , Kreig N. R. . ( 2007; ). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology, , 3rd edn., pp. 330–393. Edited by Reddy C. A. , Beveridge T. J. , Breznak J. A. , Marzluf G. , Schmidt T. M. . . Washington, DC, USA:: L. R. Snyder American Society for Microbiology;. [CrossRef]
    [Google Scholar]
  26. Wang Y. , Dai J. , Zhang L. , Luo X. , Li Y. , Chen G. , Tang Y. , Meng Y. , Fang C. . ( 2009; ). Lysobacter ximonensis sp. nov., isolated from soil. . Int J Syst Evol Microbiol 59:, 786–789. [CrossRef] [PubMed]
    [Google Scholar]
  27. Wang G.-L. , Wang L. , Chen H.-H. , Shen B. , Li S.-P. , Jiang J.-D. . ( 2011; ). Lysobacter ruishenii sp. nov., a chlorothalonil-degrading bacterium isolated from a long-term chlorothalonil-contaminated soil. . Int J Syst Evol Microbiol 61:, 674–679. [CrossRef] [PubMed]
    [Google Scholar]
  28. Wayne L. , Brenner D. , Colwell R. , Grimont P. , Kandler O. , Krichevsky M. , Moore L. , Moore W. , Murray R. et al. ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  29. Weon H.-Y. , Kim B.-Y. , Baek Y.-K. , Yoo S.-H. , Kwon S.-W. , Stackebrandt E. , Go S.-J. . ( 2006; ). Two novel species, Lysobacter daejeonensis sp. nov. and Lysobacter yangpyeongensis sp. nov., isolated from Korean greenhouse soils. . Int J Syst Evol Microbiol 56:, 947–951. [CrossRef] [PubMed]
    [Google Scholar]
  30. Weon H.-Y. , Kim B.-Y. , Kim M.-K. , Yoo S.-H. , Kwon S.-W. , Go S.-J. , Stackebrandt E. . ( 2007; ). Lysobacter niabensis sp. nov. and Lysobacter niastensis sp. nov., isolated from greenhouse soils in Korea. . Int J Syst Evol Microbiol 57:, 548–551. [CrossRef] [PubMed]
    [Google Scholar]
  31. Yassin A.-F. , Chen W.-M. , Hupfer H. , Siering C. , Kroppenstedt R.-M. , Arun A.-B. , Lai W.-A. , Shen F.-T. , Rekha P.-D. , Young C.-C. . ( 2007; ). Lysobacter defluvii sp. nov., isolated from municipal solid waste. . Int J Syst Evol Microbiol 57:, 1131–1136. [CrossRef] [PubMed]
    [Google Scholar]
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