1887

Abstract

A bacterial strain designated 6B-8 was isolated from crude oil from Daqing oilfield, China. Cells of strain 6B-8 were Gram-negative, aerobic, dimorphic and reproduced by means of binary fission. Strain 6B-8 could grow at 20–37 °C, pH 8–10 and 1–5 % (w/v) NaCl. Its genomic DNA G+C content was 62.0 mol%. The predominant cellular fatty acids were Cω7, C, C and 11-methyl Cω7 and the main hydroxy fatty acids were C 3-OH and C 3-OH when grown on marine agar 2216. The major quinone was Q-10 and the major polar lipids were three unidentified glycolipids. Phylogenetic analysis revealed that strain 6B-8 was a member of the family , sharing 99.6 and 99.4 % 16S rRNA gene sequence similarity with LMG 27140 and SLG210-30A1, respectively, and less than 94.4 % similarity with the type strains of other members of the family . However, the DNA–DNA relatedness between strain 6B-8 and related strains LMG 27140 and SLG210-30A1 was 36±5 and 42±5 %, respectively. In addition, several phenotypic and genotypic features allowed differentiation of strain 6B-8 from LMG 27140 and SLG210-30A1. Therefore, strain 6B-8 represents a novel species of genus , for which the name sp. nov. is proposed. The type strain is 6B-8 ( = CGMCC 1.12428 = LMG 27410).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31225001)
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2015-03-01
2024-10-03
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