1887

Abstract

A Gram-stain-negative, rod-shaped, facultatively anaerobic, moderately halophilic bacterium, designated strain BA45AL, was isolated from water of a saltern located in Santa Pola, Alicante, Spain. Cells were motile, and catalase- and oxidase-positive. Strain BA45AL grew at temperatures in the range 14–45 °C (optimally at 37 °C), at pH 5.0–9.0 (optimally at pH 7.5), and in media containing 5–20 % (w/v) salts [optimally in media containing 10 % (w/v) salts]. Phylogenetic analysis based on the comparison of 16S rRNA gene sequences revealed that strain BA45AL is a member of the genus . The closest relatives to the novel strain were YIM D812 and YIM D82 with sequence similarities of 98.2 % and 97.4 %, respectively. DNA–DNA hybridization between the novel isolate and these phylogenetically related species revealed relatedness values of 30 % and 15 %, respectively, with respect to the aforementioned species. The major cellular fatty acids of strain BA45AL were Cω7, C and iso-C. The G+C content of the genomic DNA of strain BA45AL was 58.0 mol%, and the polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine and a number of unknown phospholipids and lipids. Based on phenotypic, chemotaxonomic and phylogenetic data presented in this study, strain BA45AL constituted a novel species of the genus , for which the name sp. nov. is suggested. The type strain is BA45AL ( = CCM 8504 = CECT 8472 = JCM 19075 = LMG 27945).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000010
2015-03-01
2020-01-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/3/766.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000010&mimeType=html&fmt=ahah

References

  1. Bauer A. W. , Kirby W. M. M. , Sherris J. C. , Turck M. . ( 1966; ). Antibiotic susceptibility testing by a standardized single disk method. . Am J Clin Pathol 45:, 493–496.[PubMed]
    [Google Scholar]
  2. Cowan S. T. , Steel K. J. . ( 1977; ). Manual for the Identification of Medical Bacteria. London:: Cambridge University Press;.
    [Google Scholar]
  3. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376.[CrossRef]
    [Google Scholar]
  4. Fernández A. B. , Ghai R. , Martin-Cuadrado A.-B. , Sánchez-Porro C. , Rodriguez-Valera F. , Ventosa A. . ( 2014a; ). Prokaryotic taxonomic and metabolic diversity of an intermediate salinity hypersaline habitat assessed by metagenomics. . FEMS Microbiol Ecol 88:, 623–635. [CrossRef] [PubMed]
    [Google Scholar]
  5. Fernández A. B. , Vera-Gargallo B. , Sánchez-Porro C. , Ghai R. , Papke R. T. , Rodriguez-Valera F. , Ventosa A. . ( 2014b; ). Comparison of prokaryotic community structure from Mediterranean and Atlantic saltern concentrator ponds by a metagenomic approach. . Front Microbiol 5:, 196. [CrossRef] [PubMed]
    [Google Scholar]
  6. Fitch W. M. . ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  7. Ghai R. , Pašić L. , Fernández A. B. , Martin-Cuadrado A. B. , Mizuno C. M. , McMahon K. D. , Papke R. T. , Stepanauskas R. , Rodriguez-Brito B. . & other authors ( 2011; ). New abundant microbial groups in aquatic hypersaline environments. . Sci Rep 1:, 135. [CrossRef] [PubMed]
    [Google Scholar]
  8. Groth I. , Schumann P. , Weiss N. , Martin K. , Rainey F. A. . ( 1996; ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. . Int J Syst Bacteriol 46:, 234–239. [CrossRef] [PubMed]
    [Google Scholar]
  9. Johnson J. L. . ( 1994; ). Similarity analysis of DNAs. . In Methods for General and Molecular Bacteriology, pp. 655–681. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  10. Kämpfer P. , Kroppenstedt R. M. . ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42:, 989–1005. [CrossRef]
    [Google Scholar]
  11. Kim O.-S. , Cho Y.-J. , Lee K. , Yoon S.-H. , Kim M. , Na H. , Park S.-C. , Jeon Y. S. , Lee J.-H. . & other authors ( 2012; ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  12. León M. J. , Fernández A. B. , Ghai R. , Sánchez-Porro C. , Rodriguez-Valera F. , Ventosa A. . ( 2014; ). From metagenomics to pure culture: isolation and characterization of the moderately halophilic bacterium Spiribacter salinus gen. nov., sp. nov.. Appl Environ Microbiol 80:, 3850–3857. [CrossRef] [PubMed]
    [Google Scholar]
  13. Marmur J. . ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  14. Marmur J. , Doty P. . ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5:, 109–118. [CrossRef] [PubMed]
    [Google Scholar]
  15. Mellado E. , Moore E. R. B. , Nieto J. J. , Ventosa A. . ( 1995; ). Phylogenetic inferences and taxonomic consequences of 16S ribosomal DNA sequence comparison of Chromohalobacter marismortui, Volcaniella eurihalina, and Deleya salina and reclassification of V. eurihalina as a Halomonas eurihalina comb. nov. . Int J Syst Bacteriol 45:, 712–716.[CrossRef]
    [Google Scholar]
  16. Pfennig N. , Trüper H. G. . ( 1971; ). Higher taxa of the phototrophic bacteria. . Int J Syst Bacteriol 21:, 17–18.[CrossRef]
    [Google Scholar]
  17. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  18. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
  19. Stackebrandt E. , Goebel B. M. . ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  20. Subov, N. N. (1931). Oceanographical tables. Comissariat of Agriculture of URSS, Hydro-Meteorological Commitee of URSS, Oceanographical Institute of URSS, Moscow.
  21. Tindall B. J. , Sikorski J. , Smibert R. A. , Krieg N. R. . ( 2007; ). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology, pp. 330–393. Edited by Reddy C. A. , Beveridge T. J. , Breznak J. A. , Marzluf G. A. , Schmidt T. M. , Snyder R. L. . . Washington, D. C.:: American Society for Microbiology;. [CrossRef]
    [Google Scholar]
  22. Ventosa A. , Quesada E. , Rodríguez-Valera F. , Ruiz-Berraquero F. , Ramos-Cormenzana A. . ( 1982; ). Numerical taxonomy of moderately halophilic Gram-negative rods. . J Gen Microbiol 128:, 1959–1968.
    [Google Scholar]
  23. Ventosa A. , Gutiérrez M. C. , Kamekura M. , Zvyagintseva I. S. , Oren A. . ( 2004; ). Taxonomic study of Halorubrum distributum and proposal of Halorubrum terrestre sp. nov.. Int J Syst Evol Microbiol 54:, 389–392. [CrossRef] [PubMed]
    [Google Scholar]
  24. Ventosa A. , Fernández A. B. , León M. J. , Sánchez-Porro C. , Rodriguez-Valera F. . ( 2014; ). The Santa Pola saltern as a model for studying the microbiota of hypersaline environments. . Extremophiles 18:, 811–824. [CrossRef] [PubMed]
    [Google Scholar]
  25. Wang Y. X. , Liu J.-H. , Zhang X. X. , Chen Y.-G. , Wang Z. G. , Chen Y. , Li Q. Y. , Peng Q. , Cui X. L. . ( 2009; ). Fodinicurvata sediminis gen. nov., sp. nov. and Fodinicurvata fenggangensis sp. nov., poly-β-hydroxybutyrate-producing bacteria in the family Rhodospirillaceae . . Int J Syst Evol Microbiol 59:, 2575–2581. [CrossRef] [PubMed]
    [Google Scholar]
  26. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. . & other authors ( 1987; ). International Committee on Systematic Bacteriology. R eport of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000010
Loading
/content/journal/ijsem/10.1099/ijs.0.000010
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error