1887

Abstract

A Gram-stain-negative, rod-shaped, facultatively anaerobic, moderately halophilic bacterium, designated strain BA45AL, was isolated from water of a saltern located in Santa Pola, Alicante, Spain. Cells were motile, and catalase- and oxidase-positive. Strain BA45AL grew at temperatures in the range 14–45 °C (optimally at 37 °C), at pH 5.0–9.0 (optimally at pH 7.5), and in media containing 5–20 % (w/v) salts [optimally in media containing 10 % (w/v) salts]. Phylogenetic analysis based on the comparison of 16S rRNA gene sequences revealed that strain BA45AL is a member of the genus . The closest relatives to the novel strain were YIM D812 and YIM D82 with sequence similarities of 98.2 % and 97.4 %, respectively. DNA–DNA hybridization between the novel isolate and these phylogenetically related species revealed relatedness values of 30 % and 15 %, respectively, with respect to the aforementioned species. The major cellular fatty acids of strain BA45AL were Cω7, C and iso-C. The G+C content of the genomic DNA of strain BA45AL was 58.0 mol%, and the polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine and a number of unknown phospholipids and lipids. Based on phenotypic, chemotaxonomic and phylogenetic data presented in this study, strain BA45AL constituted a novel species of the genus , for which the name sp. nov. is suggested. The type strain is BA45AL ( = CCM 8504 = CECT 8472 = JCM 19075 = LMG 27945).

Funding
This study was supported by the:
  • , Spanish Ministry of Science and Innovation , (Award CGL2013-46941-P and BIO2011-12879-E)
  • , Junta de Andalucía , (Award P10-CVI-6226)
  • , FEDER
  • , lan Andaluz de Investigación
  • , Spanish Ministry of Science and Innovation
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000010
2015-03-01
2021-03-04
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/3/766.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000010&mimeType=html&fmt=ahah

References

  1. Bauer A. W., Kirby W. M. M., Sherris J. C., Turck M. ( 1966 ). Antibiotic susceptibility testing by a standardized single disk method. . Am J Clin Pathol 45, 493496.[PubMed]
    [Google Scholar]
  2. Cowan S. T., Steel K. J. ( 1977 ). Manual for the Identification of Medical Bacteria. London:: Cambridge University Press;.
    [Google Scholar]
  3. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [CrossRef]
    [Google Scholar]
  4. Fernández A. B., Ghai R., Martin-Cuadrado A.-B., Sánchez-Porro C., Rodriguez-Valera F., Ventosa A. ( 2014a ). Prokaryotic taxonomic and metabolic diversity of an intermediate salinity hypersaline habitat assessed by metagenomics. . FEMS Microbiol Ecol 88, 623635. [CrossRef] [PubMed]
    [Google Scholar]
  5. Fernández A. B., Vera-Gargallo B., Sánchez-Porro C., Ghai R., Papke R. T., Rodriguez-Valera F., Ventosa A. ( 2014b ). Comparison of prokaryotic community structure from Mediterranean and Atlantic saltern concentrator ponds by a metagenomic approach. . Front Microbiol 5, 196. [CrossRef] [PubMed]
    [Google Scholar]
  6. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406416. [CrossRef]
    [Google Scholar]
  7. Ghai R., Pašić L., Fernández A. B., Martin-Cuadrado A. B., Mizuno C. M., McMahon K. D., Papke R. T., Stepanauskas R., Rodriguez-Brito B. & other authors ( 2011 ). New abundant microbial groups in aquatic hypersaline environments. . Sci Rep 1, 135. [CrossRef] [PubMed]
    [Google Scholar]
  8. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. ( 1996 ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. . Int J Syst Bacteriol 46, 234239. [CrossRef] [PubMed]
    [Google Scholar]
  9. Johnson J. L. ( 1994 ). Similarity analysis of DNAs. . In Methods for General and Molecular Bacteriology, pp. 655681. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  10. Kämpfer P., Kroppenstedt R. M. ( 1996 ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42, 9891005. [CrossRef]
    [Google Scholar]
  11. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [CrossRef] [PubMed]
    [Google Scholar]
  12. León M. J., Fernández A. B., Ghai R., Sánchez-Porro C., Rodriguez-Valera F., Ventosa A. ( 2014 ). From metagenomics to pure culture: isolation and characterization of the moderately halophilic bacterium Spiribacter salinus gen. nov., sp. nov.. Appl Environ Microbiol 80, 38503857. [CrossRef] [PubMed]
    [Google Scholar]
  13. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3, 208218. [CrossRef]
    [Google Scholar]
  14. Marmur J., Doty P. ( 1962 ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5, 109118. [CrossRef] [PubMed]
    [Google Scholar]
  15. Mellado E., Moore E. R. B., Nieto J. J., Ventosa A. ( 1995 ). Phylogenetic inferences and taxonomic consequences of 16S ribosomal DNA sequence comparison of Chromohalobacter marismortui, Volcaniella eurihalina, and Deleya salina and reclassification of V. eurihalina as a Halomonas eurihalina comb. nov. . Int J Syst Bacteriol 45, 712716. [CrossRef]
    [Google Scholar]
  16. Pfennig N., Trüper H. G. ( 1971 ). Higher taxa of the phototrophic bacteria. . Int J Syst Bacteriol 21, 1718. [CrossRef]
    [Google Scholar]
  17. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  18. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
  19. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846849. [CrossRef]
    [Google Scholar]
  20. Subov, N. N. (1931). Oceanographical tables. Comissariat of Agriculture of URSS, Hydro-Meteorological Commitee of URSS, Oceanographical Institute of URSS, Moscow.
  21. Tindall B. J., Sikorski J., Smibert R. A., Krieg N. R. ( 2007 ). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology, pp. 330393. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G. A., Schmidt T. M., Snyder R. L. . Washington, D. C.:: American Society for Microbiology;. [CrossRef]
    [Google Scholar]
  22. Ventosa A., Quesada E., Rodríguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A. ( 1982 ). Numerical taxonomy of moderately halophilic Gram-negative rods. . J Gen Microbiol 128, 19591968.
    [Google Scholar]
  23. Ventosa A., Gutiérrez M. C., Kamekura M., Zvyagintseva I. S., Oren A. ( 2004 ). Taxonomic study of Halorubrum distributum and proposal of Halorubrum terrestre sp. nov.. Int J Syst Evol Microbiol 54, 389392. [CrossRef] [PubMed]
    [Google Scholar]
  24. Ventosa A., Fernández A. B., León M. J., Sánchez-Porro C., Rodriguez-Valera F. ( 2014 ). The Santa Pola saltern as a model for studying the microbiota of hypersaline environments. . Extremophiles 18, 811824. [CrossRef] [PubMed]
    [Google Scholar]
  25. Wang Y. X., Liu J.-H., Zhang X. X., Chen Y.-G., Wang Z. G., Chen Y., Li Q. Y., Peng Q., Cui X. L. ( 2009 ). Fodinicurvata sediminis gen. nov., sp. nov. and Fodinicurvata fenggangensis sp. nov., poly-β-hydroxybutyrate-producing bacteria in the family Rhodospirillaceae . . Int J Syst Evol Microbiol 59, 25752581. [CrossRef] [PubMed]
    [Google Scholar]
  26. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. R eport of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000010
Loading
/content/journal/ijsem/10.1099/ijs.0.000010
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error