1887

Abstract

A Gram-stain-negative, rod-shaped, facultatively anaerobic, moderately halophilic bacterium, designated strain BA45AL, was isolated from water of a saltern located in Santa Pola, Alicante, Spain. Cells were motile, and catalase- and oxidase-positive. Strain BA45AL grew at temperatures in the range 14–45 °C (optimally at 37 °C), at pH 5.0–9.0 (optimally at pH 7.5), and in media containing 5–20 % (w/v) salts [optimally in media containing 10 % (w/v) salts]. Phylogenetic analysis based on the comparison of 16S rRNA gene sequences revealed that strain BA45AL is a member of the genus . The closest relatives to the novel strain were YIM D812 and YIM D82 with sequence similarities of 98.2 % and 97.4 %, respectively. DNA–DNA hybridization between the novel isolate and these phylogenetically related species revealed relatedness values of 30 % and 15 %, respectively, with respect to the aforementioned species. The major cellular fatty acids of strain BA45AL were Cω7, C and iso-C. The G+C content of the genomic DNA of strain BA45AL was 58.0 mol%, and the polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine and a number of unknown phospholipids and lipids. Based on phenotypic, chemotaxonomic and phylogenetic data presented in this study, strain BA45AL constituted a novel species of the genus , for which the name sp. nov. is suggested. The type strain is BA45AL ( = CCM 8504 = CECT 8472 = JCM 19075 = LMG 27945).

Funding
This study was supported by the:
  • Spanish Ministry of Science and Innovation (Award CGL2013-46941-P and BIO2011-12879-E)
  • Junta de Andalucía (Award P10-CVI-6226)
  • FEDER
  • lan Andaluz de Investigación
  • Spanish Ministry of Science and Innovation
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2015-03-01
2024-03-29
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