RT Journal Article SR Electronic(1) A1 Al-Zarban, Sheikha S A1 Abbas, Ibrahim A1 Al-Musallam, Azza A A1 Steiner, Ulrike A1 Stackebrandt, Erko A1 Kroppenstedt, Reiner MYR 2002 T1 Nocardiopsis halotolerans sp. nov., isolated from salt marsh soil in Kuwait. JF International Journal of Systematic and Evolutionary Microbiology, VO 52 IS 2 SP 525 OP 529 DO https://doi.org/10.1099/00207713-52-2-525 PB Microbiology Society, SN 1466-5034, AB A polyphasic taxonomic study of a halotolerant micro-organism, isolated from Kuwait salt marsh soil, revealed that this strain represents a novel Nocardiopsis species. The strain produced substrate and aerial mycelium, grew at 28-35 degrees C in salt concentrations of 0-15% and was slightly keratinolytic. Results of the 165 rDNA sequence comparison revealed that strain F100T clustered with strains of the genus Nocardiopsis. This is consistent with other data such as: (i) growth characteristics, i.e. the formation of a white to yellow aerial mycelium and the typical zig-zag form of hyphae, which fragment when ageing; (ii) the presence of DL-diaminopimelic acid and glucose plus ribose in whole-cell hydrolysates; (iii) the presence of phosphatidyl choline, phosphatidyl inositol, phosphatidyl glycerol, phosphatidyl methylethanolamine and diphosphatidyl glycerol in polar lipid extracts; (iv) the presence of menaquinones MK-10(H(0-6)) and MK-11(H(0-6)) in the non-polar fraction; (v) the presence of iso/anteiso-branched plus 10-methyl-branched fatty acids, showing the diagnostic combination for Nocardiopsis spp. of 14-methyl-hexadecanoic acid (18%), oleic acid (9%) and tuberculostearic acid (2%); and (v) the absence of mycolic acids. Analysis of 16S rDNA revealed that strain F100T represents a distinct taxon within Nocardiopsis. Based upon phenotypic differences to other members of the genus, a novel species, Nocardiopsis halotolerans sp. nov., is proposed. The type strain of the species is F100T (= DSM 44410T = NRRL B-24124T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-52-2-525