%0 Journal Article %A Xin, H %A Itoh, T %A Zhou, P %A Suzuki, K %A Nakase, T %T Natronobacterium nitratireducens sp. nov., a aloalkaliphilic archaeon isolated from a soda lake in China. %D 2001 %J International Journal of Systematic and Evolutionary Microbiology, %V 51 %N 5 %P 1825-1829 %@ 1466-5034 %R https://doi.org/10.1099/00207713-51-5-1825 %I Microbiology Society, %X Two novel haloalkaliphilic archaea, strains C231T and C42, were isolated from a soda lake in China. Cells of the two strains were rod-shaped and gram-negative and colonies were bright red. They required at least 2.5 M NaCl for growth, with an optimum at 3.5 M NaCl, and grew over a pH range from 8.0 to 10.5, with an optimum at pH 8.5. Hypotonic treatment with less than 1.5 M NaCl caused cell lysis. They had similar polar lipid compositions, possessing the diphytanyl (C20:C20) and phytanyl-sesterterpanyl (C20:C25) diether derivatives of phosphatidylglycerol and phosphatidylglycerophosphate methyl ester and a minor phospholipid, PL1. No glycolipids were detected. Comparison of 16S rDNA sequences and morphological features placed them in the genus Natronobacterium. Detailed phenotypic characterization and DNA-DNA hybridization studies revealed that the two strains belong to a new species in the genus Natronobacterium, for which the name Natronobacterium nitratireducens sp. nov. is proposed. The type strain is C231T (= AS 1.1980T = JCM 10879T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-51-5-1825