sp. nov., isolated from the intestine of Atlantic salmon () Free

Abstract

Strain K1, isolated from the gastrointestinal tract of Atlantic salmon (), has the capacity to inhibit the growth of the fish pathogens and Strain K1 is a motile Gram-positive psychrophilic rod that lacks both catalase and oxidase, which does not grow on acetate containing media, but grows at pH 9 and in TSB with up to 6 % sodin chloride content. Strain K1 is facultatively anaerobic and tryptone as a sole source of nutrient promotes growth. The most abundant cellular fatty acid of strain K1 is oleic acid (18:19). Based on 16S rDNA sequence comparisons, it is suggested that strain K1 is phylogenetically closely related to C. However, the unique phenotypic attributes of strain K1 suggest that it represents a new species. The name i proposed, for which the type strain is K1 (= CCUG 31728).

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1999-10-01
2024-03-28
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References

  1. Axelsson L. T. 1993 Lactic acid bacteria: classification and physiology. Lactic Acid Bacteria1–63 Edited by Salminen A., von Wright A. New York: Marcel Dekker;
    [Google Scholar]
  2. Baya A. M., Toranzo A. E., Lupiani B., Li T., Roberson B. S., Hetrick F. M. 1991; Biochemical and serological characterization of Carnobacterium spp. isolated from farmed and natural populations of striped bass and catfish. Appl Environ Microbiol 57:3114–3120
    [Google Scholar]
  3. Brosius G., Palmer J. L., Kennedy J. P., NoIler H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Sci USA 75:4801–4805
    [Google Scholar]
  4. Collins M. D., Farrow J. A. E., Phillips B. A., Ferusu S., Jones D. 1987; Classification of Lactobacillus divergens, Lactobacillus piscicola, and some catalase-negative, asporogenous, rodshaped bacteria from poultry in a new genus, Carnobacterium. Int J Syst Bacteriol 37:310–316
    [Google Scholar]
  5. Dorsch M., Stackebrandt E. 1992; Some modifications in the procedure of direct sequencing of PCR amplified 16S rDNA. J Microbiol Methods 16:271–279
    [Google Scholar]
  6. Evelyn T. P. T., McDermott L. A. 1961; Bacteriological studies of fresh water fish. Can J Microbiol 7:375–382
    [Google Scholar]
  7. Franzmann P. D., Hbpfl P., Weiss N., Tindall B. J. 1991; Psychrophilic, lactic acid-producing bacteria from anoxic waters in Ace Lake, Antartica; Carnobacterium funditum sp. nov. and Carnobacterium alterfunditum sp. nov. Arch Microbiol 156:255–262
    [Google Scholar]
  8. Heimbrook M. E., Wang W. L. L., Campbell G. 1989; Staining bacterial flagella easily. J Clin Microbiol 27:2612–2615
    [Google Scholar]
  9. Hiu S. F., Holt R. A., Sriranganathan N., Seidler R. J., Fryer J. L. 1984; Lactobacilluspiscicola, a new species from salmonid fish. Int J Syst Bacteriol 34:393–400
    [Google Scholar]
  10. Humphrey J. D., Lancaster C. E., Gudkovs N., Copland J. W. 1987; The disease status of Australian salmonids: bacteria and bacterial diseases. J Fish Dis 10:403–410
    [Google Scholar]
  11. Jóborn A., Olsson C, Westerdahl A., Conway P., Kjelleberg S. 1997; Colonisation in the fish intestinal tract and production of inhibitory substances in intestinal mucus and faecal extracts by Carnobacterium sp. strain KI. J Fish Dis 20:383–392
    [Google Scholar]
  12. Knochel S. 1981; Occurence and some characteristics of Lactobacillus spp. isolated from fish. J Appl Bacteriol 51:10
    [Google Scholar]
  13. Kvasnikov E. I., Kovalenko N. K., Materinskaya L. G. 1977; Lactic acid bacteria of freshwater fish. Mikrobiologiya 46:619–624 in Russian
    [Google Scholar]
  14. Leif son E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  15. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  16. Márdén P., Tunlid A., Malmcrona-Friberg K., Odham G., Kjelleberg S. 1985; Physiological and morphological changes during short term starvation of marine bacterial isolates. Arch Microbiol 142:326–332
    [Google Scholar]
  17. Montel M.-C, Talon R., Fournaud J., Champomier M.-C. 1991; A simplified key for identifying homofermentative Lactobacillus and Carnobacterium spp. from meat. J Appl Bacteriol 70:469–472
    [Google Scholar]
  18. Óstling J., Goodman A., Kjelleberg S. 1991; Behaviour of IncP-1 plasmids and a miniMu transposon in a marine Vibrio sp.: isolation of and starvation inducible lac operon fusions. FEMS Microbiol Ecol 86:83–94
    [Google Scholar]
  19. Rainey F. A., Dorsch M., Morgan H. W., Stackebrandt E. 1992; 16S rDNA analysis of Spirochaeta thermophila’. Its phylogenetic position and implication for the systematics of the order Spirochaetales. Syst Appl Microbiol 15:197–202
    [Google Scholar]
  20. Ringo E. 1993; Arctic charr, Salvelinus alpinus (L.), reared in fresh and sea water. PhD thesis University of Tromso;
    [Google Scholar]
  21. Ross A. J., Toth R. J. 1974; Lactobacillus - a new fish pathogen. Prog Fish Cult 36:191
    [Google Scholar]
  22. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  23. Schrbder K., Clausen E., Sandberg A. M., Raa J. 1980 Psychrophilic Lactobacillus plantarum from fish and its ability to produce antibiotic substances. Advances in Fish Science and Technology480–483 Edited by Connell J. J. Farnham, Surrey: Fishing News Books;
    [Google Scholar]
  24. Spielmeyer W., McMeekin T. A., Miller J., Franzmann P. D. 1993; Phylogeny of the Antarctic bacterium, Carnobacterium alterfunditum. Polar Biol 13:501–503
    [Google Scholar]
  25. Strom E. 1988 Melkesyrebakterier i fisketarm. Isolasjon, karakterisering og egenskaper Licentiate treatise University of Tromso;
    [Google Scholar]
  26. Wallbanks S., Martinez-Murcia A. J., Fryer J. L, Phillips A. B., Collins M. D. 1990; 16S rRNA sequence determination for members of the genus Carnobacterium and related Lactic acid bacteria and description of Vagococcus salmonarium sp. nov. Int J Syst Bacteriol 40:224–230
    [Google Scholar]
  27. Westerdahl A., Olsson J. C., Kjelleberg S., Conway P. L. 1991; Isolation and characterization of turbot (Scophthalmus maximus)-associated bacteria with inhibitory effect against Vibrio anguillarum. Appl Environ Microbiol 57:2223–2228
    [Google Scholar]
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