sp. nov., a rubber-degrading actinomycete isolated from an automobile tyre Free

Abstract

A rubber-degrading bacterium (strain Kd2) was isolated from fouling tyre water inside a deteriorated automobile tyre. The strain was aerobic, Grampositive, produced elementary branching hyphae which fragmented into rod/coccus-like elements and showed chemotaxonomic markers which were consistent with the classification of , i.e. -diaminopimelic acid, -glycolyl muramic acid, arabinose and galactose as diagnostic sugars, a fatty acid pattern composed of unbranched saturated and monounsaturated fatty acids with a considerable amount of tuberculostearic acid, and mycolic acids comprising 58-66 carbon atoms with two principal mycolic acids C and C counting for over 60%. Results of 16S rDNA analyses as well as chemotaxonomic results, led to the conclusion that sp. strain Kd2 (= DSM 44302) represents a new species within the genus for which the name is proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-49-4-1785
1999-10-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/49/4/ijs-49-4-1785.html?itemId=/content/journal/ijsem/10.1099/00207713-49-4-1785&mimeType=html&fmt=ahah

References

  1. Bell K. S., Philip J. C., Aw D. W. J., Christofi N. 1998; The genus Rhodococcus. J Appl Microbiol 85:195–210
    [Google Scholar]
  2. Bendinger B., Rainey F. A., Kroppenstedt R. M., Moormann M., Klatte S. 1995; Gordonia hydrophobica sp. nov., isolated from biofilters for waste gas treatment. Int J Syst Bacteriol 45:544–548
    [Google Scholar]
  3. De Soete G. 1983; A least squares algorithm for fitting additive trees to proximity data. Psychometrika 48:621–626
    [Google Scholar]
  4. Goodfellow M. 1986 Genus Rhodococcus Zopf 1891, 28AL. Bergey’s Manual of Systematic Bacteriology 42362–2371 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  5. Goodfellow M. 1992 The family Nocardiaceae. The Prokaryotes, 2nd.1188–1213 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer;
    [Google Scholar]
  6. Goodfellow M., Lechevalier M. P. 1989 Genus Nocardia. Trevisan 1899, 9AL. Bergey’s Manual of Systematic Bacteriology 42350–2361 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  7. Heferan M. 1904; A comparative and experimental study of bacilli producing red pigment. Zbl Bakteriol Parasitenk Infektionskr Hyg Abt II 2:397–404
    [Google Scholar]
  8. Jukes T. H., Cantor C. R. 1969 Evolution of protein molecules. Mammalian Protein Metabolism 321–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  9. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005
    [Google Scholar]
  10. Kämpfer P., Dott W., Kroppenstedt R. M. 1990; Numerical classification and numerical identification of some nocardioform bacteria. J Gen Appl Microbiol 36:309–331
    [Google Scholar]
  11. Kirchhof H., Maas C., Runge M., Franz B., Schmidt R., Quentmeier H., Mühlhardt R. F. 1992; Tetrazolium-[3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] reduction by mycoplasmas. Int J Syst Bacteriol 42:506–508
    [Google Scholar]
  12. Klatte S. 1994; Polyphasische automatisierte Identifizierung der Gattungen Corynebacterium Lehmann & Neumann 1896 und Rhodococcus Zopf 1891. PhD thesis University Osnabrück;
    [Google Scholar]
  13. Klatte S., Kroppenstedt R. M., Rainey F. A. 1994a; Rhodococcus opacus sp. nov. An unusual nutritionally versatile Rhodococcus-spzcies. Syst Appl Microbiol 17:355–360
    [Google Scholar]
  14. Klatte S., Rainey F. A., Kroppenstedt R. M. 1994b; Transfer of Rhodococcus aichiensis Tsukamurella 1982 and Nocardia amarae Lechevalier and Lechevalier 1974 to the genus Gor dona as Gordona aichiensis comb. nov. and Gordona amarae comb nov. Int J Syst Bacteriol 44:769–773
    [Google Scholar]
  15. Klatte S., Kroppenstedt R. M., Schumann P., Altendorf K.-H., Rainey F. A. 1996; Gordona hirsuta sp. nov. Int J Syst Bacteriol 46:876–880
    [Google Scholar]
  16. Kroppenstedt R. M. 1982; Separation of bacterial menaquinones by HPLC using reverse phase (RP-18) and a silver loaded ion exchanger. J Liquid Chromatogr 5:2359–2367
    [Google Scholar]
  17. Kroppenstedt R. M. 1985 Fatty acid and menaquinone analysis of actinomycetes and related organisms. Chemical Methods in Bacterial Systematics, no. 20 SAB Technical Series173–199 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  18. Lechevalier H. A., Lechevalier M. P. 1970 A critical evaluation of the genera of aerobic actinomycetes. The Actinomycetales393–405 Edited by Prauser H. Jena: Gustav Fischer Verlag;
    [Google Scholar]
  19. Lechevalier M. P., Lechevalier H. A. 1974; Nocardia amarae sp. nov., an actinomycete common in foaming activated sludge. Int J Syst Bacteriol 24:278–288
    [Google Scholar]
  20. Lefford M. J. 1980 Mycobacterial Culture Collection, Catalogue of strains. U S Department of Health and Human Services. Public Health Service. National Institutes of Health; NIH Publication no. 80-289
    [Google Scholar]
  21. Lemmer H., Kroppenstedt R. M. 1984; Chemotaxonomy and physiology of some actinomycetes isolated from scumming activated sludge. Syst Appl Microbiol 5:124–135
    [Google Scholar]
  22. Linos A., Steinbüchel A. 1996; Investigations on the microbial breakdown of natural and synthetic rubbers. DECHEMA Monogr 133:279–285
    [Google Scholar]
  23. Linos A., Steinbüchel A. 1998; Microbial degradation of natural and synthetic rubbers by novel bacteria belonging to the genus Gordona. Kautsch Gummi Kunstst 51:496–499
    [Google Scholar]
  24. Maidak B. L, Larsen N., McGaughey M. J., Over bee k R., Olsen G. J., Fogel K., Blandy J., Woese C. R. 1994; The Ribosomal Database Project. Nucleic Acids Res 22:3485–3487
    [Google Scholar]
  25. Maidak B. L, Olsen G. J., Larsen N., McGaughey M. J., Woese C. R. 1996; The ribosomal data project (RDP). Nucleic Acids Res 84:82–85
    [Google Scholar]
  26. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson A., Athalye M., Schaal K., Parlett J. H. 1984; An integrated procedure for the extraction of isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241
    [Google Scholar]
  27. Minnikin D. E., Patel V., Alshamaony L., Goodfellow M. 1977; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117
    [Google Scholar]
  28. Miller L. T. 1982; A single derivatization method for bacterial fatty acid methyl esters including hydroxy acids. J Clin Microbiol 16:584–586
    [Google Scholar]
  29. Rainey F. A., Burghardt J., Kroppenstedt R. M., Klatte S., Stackebrandt E. 1995; Phylogenetic analysis of the genera Rhodococcus and Nocardia and evidence for the evolutionary origin of the genus Nocardia from within the radiation of Rhodococcus species. Microbiology 141:523–528
    [Google Scholar]
  30. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: Proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092
    [Google Scholar]
  31. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsletter 20:1–6
    [Google Scholar]
  32. Sodell J. A., Seviour R. J. 1995; Relationship between temperature and growth of organisms causing Nocardia foams in activated sludge plants. Water Res 29:1555–1558
    [Google Scholar]
  33. Stanek J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  34. Stackebrandt E., Smida J., Gollins M. D. 1988; Evidence of phylogenetic heterogeneity within the genus Rhodococcus: revival of the genus Gordona (Tsukamura). J Gen Appl Microbiol 34:343–348
    [Google Scholar]
  35. Stackebrandt E., Rainey A. F., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491
    [Google Scholar]
  36. Takeuchi M., Hatano K. 1998; Gordonia rhizosphera sp. nov., isolated from the mangrove rhizosphere. Int J Syst Bacteriol 48:907–912
    [Google Scholar]
  37. Tsukamura M. 1971; Proposal of a new genus, Gordona, for slightly acid fast organisms occurring in sputi of patients with pulmonary disease and in soil. J Gen Microbiol 68:15–26
    [Google Scholar]
  38. Tsukamura M. 1978; Numerical classification of Rhodococcus (formerly Gordona) organisms recently isolated from sputi of patients: description of Rhodococcus sputi Tsukamura sp. nov. Int J Syst Bacteriol 28:169–181
    [Google Scholar]
  39. Tsukamura M. 1982; Numerical analysis of the taxonomy of nocardiae and rhodococci. Division of Nocardia asteroides sensu stricto into two species and description of Nocardia paratuberculosis sp. nov. Tsukamura (formerly the Kyoto-I group of Tsukamura), Nocardia nova sp. nov. Tsukamura, Rhodococcus aichiensis sp. nov. Tsukamura, Rhodococcus chubunensis sp. nov. Tsukamura, and Rhodococcus obuensis sp. nov. Tsukamura. Microbiol Immunol 26:1101–1119
    [Google Scholar]
  40. Uchida K., Aida K. 1977; Acyl type of bacterial cell wall: its simple identification by a colorimetric method. J Gen Appl Microbiol 23:249–260
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-49-4-1785
Loading
/content/journal/ijsem/10.1099/00207713-49-4-1785
Loading

Data & Media loading...

Most cited Most Cited RSS feed