1887

Abstract

A strictly chemo-organotrophic, anaerobic bacterium was isolated from an olive mill wastewater treatment digester on syringate and designated strain SR1. The cells were slightly curved rods, stained Gram-positive and possessed terminal spores. Strain SR1 utilized crotonate, methanol and a wide range of aromatic compounds including 3,4,5-trimethoxybenzoate (TMB), 3,4,5-trimethoxycinnamate (TMC), syringate, 3,4,5-trimethoxyphenylacetate (TMPA), 3,4,5-trimethoxyphenylpropionate (TMPP), ferulate, sinapate, vanillate, 3,4-dimethoxybenzoate, 2,3-dimethoxybenzoate, gallate, 2,4,6-trihydroxybenzoate (THB), pyrogallol, phloroglucinol and quercetin as carbon and energy sources. Acetate and butyrate were produced from aromatic compounds, methanol and crotonate whereas methanethiol (MT) was produced from methoxylated aromatic compounds and methanol. Strain SR1 had a G+C content of 38 mol% and grew optimally between 37 and 40 °C at pH 7·2 on a crotonate-containing medium. Phylogenetically, strain SR1 was a member of cluster XIVa of the group and shared a sequence similarity of 90% with and . Consequently, its precise neighbourliness to any one of them depended on the selection of strains of the cluster. On the basis of the phylogenetic and phenotypic evidence presented in this paper, the designation of strain SR1 as gen. nov., sp. nov. is proposed. The type strain is SR1 (= DSM 12504).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-49-4-1741
1999-10-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/49/4/ijs-49-4-1741.html?itemId=/content/journal/ijsem/10.1099/00207713-49-4-1741&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Madden T. L., Schaffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402
    [Google Scholar]
  2. Andrews K. T., Patel B. K. C. 1996; Fervidobacterium gondwanense sp. nov., a new thermophilic anaerobic bacterium isolated from nonvolcanically heated geothermal waters of the Great Artesian Basin of Australia. Int J Syst Bacteriol 46:265–269
    [Google Scholar]
  3. Bak F., Finster K., RothfuB F. 1992; Formation of dimethylsulfide and methanethiol from methoxylated aromatic compounds and inorganic sulfide by newly isolated anaerobic bacteria. Arch Microbiol 157:529–534
    [Google Scholar]
  4. Benson D., Lipman D. J., Ostell J. 1993; GenBank. Nucleic Acids Res 21:2963–2965
    [Google Scholar]
  5. Brauman A., Muller J. A., Garcia J.-L., Brune A., Schink B. 1998; Fermentative degradation of 3-hydroxybenzoate in pure culture by a novel strictly anaerobic bacterium, Sporotomaculum hydroxybenzoicum gen. nov., sp. nov. Int J Syst Bacteriol 48:215–221
    [Google Scholar]
  6. Capasso R., Evidente A., Schivo L., Orru G., Marcialis M. A., Cristinzio G. 1995; Antibacterial polyphenols from olive oil mill waste waters. J Appl Bacteriol 79:393–398
    [Google Scholar]
  7. Collins M. D., Lawson P. A., Willems A., Cordoba J. J., Fernandez-Garayzabal J., Garcia P., Cai J., Hippe H., Farrow J. A. E. 1994; The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 44:812–826
    [Google Scholar]
  8. Fardeau M.-L., Ollivier B., Patel B. K. C., Magot M., Thomas P., Rimbault A., Rocchiccioli F., Garcia J.-L. 1997; Thermotoga hypogea sp. nov., a xylanolytic, thermophilic bacterium from an oil-producing well. Int J Syst Bacteriol 47:1013–1019
    [Google Scholar]
  9. Felsenstein J. 1993 phylip (Phylogeny Inference Package) version 3 < PT > 51c. Department of Genetics, University of Washington; Seattle, USA:
    [Google Scholar]
  10. Grech-Mora I., Fardeau M.-L., Patel B. K. C, Ollivier B., Rimbault A., Prensier G., Garcia J.-L., Garnier-Sillam E. 1996; Isolation and characterization of Sporobacter termitidis gen. nov., sp. nov., from the digestive tract of the wood-feeding termite Nasutitremes lujae. Int J Syst Bacteriol 46:512–518
    [Google Scholar]
  11. Hardman J. K., Stadtman T. C. 1960; Metabolism of coamino acid. II. fermentation of A-aminovaleric acid by Clostridium aminovalericum. J Bacteriol 79:549–552
    [Google Scholar]
  12. Hungate R. E. 1969; A roll-tube method for the cultivation of strict anaerobes. Methods Microbiol 136:194–198
    [Google Scholar]
  13. Jukes T. H., Cantor C. R. 1969 Evolution of protein molecules. Mammalian Protein Metabolism211–232 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  14. Krumholz L. R., Bryant M. P. 1986; Eubacterium oxidoreducens sp. nov. requiring H, or formate to degrade gallate, pyrogallol, phloroglucinol and quercetin. Arch Microbiol 144:8–14
    [Google Scholar]
  15. Liesack W., Bak F., Kreft J. U., Stackebrandt E. 1994; Holophaga foetida gen. nov., sp. nov., a new homoacetogenic bacterium degrading methoxylated aromatic compounds. Arch Microbiol 162:85–90
    [Google Scholar]
  16. Macy J. M., Snellen J. E., Hungate R. E. 1972; Use of syringe methods for anerobiosis. Am J Clin Nutr 25:1318–1323
    [Google Scholar]
  17. Maidak B. L., Olsen G. J., Larsen N., Overbeek R., McGaughey M. J., Woese C. R. 1997; The RDP (Ribosomal Database Project). Nucleic Acids Res 25:109–111
    [Google Scholar]
  18. Mechichi T., Labat M., Patel B. K. C., Woo T. H. S., Thomas P., Garcia J.-L. 1999; Clostridium methoxybenzovorans sp. nov., a new aromatic o-demethylating homoacetogen from an olive mill wastewater treatment digester. Int J Syst Bacteriol 49:1201–1209
    [Google Scholar]
  19. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G + C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167
    [Google Scholar]
  20. Miller T. L., Wolin M. J. 1974; A serum bottle modification of the Hungate technique for cultivating obligate anaerobes. Appl Microbiol 27:985–987
    [Google Scholar]
  21. Patel T. R., Jure K. G., Jones G. A. 1981; Catabolism of phloroglucinol by the rumen anaerobe Coprococcus. Appl Environ Microbiol 42:1010–1017
    [Google Scholar]
  22. Redburn A. C., Patel B. K. C. 1993; Phylogenetic analysis of Desulfotomaculum thermobenzoicum using polymerase chain reaction-amplified 16S rRNA-specific DNA. FEMS Microbiol Lett 113:81–86
    [Google Scholar]
  23. Schink B., Pfennig N. 1982; Fermentation of trihydroxybenzenes by Pelobacter acidigallici gen. nov., sp. nov., a new strictly anaerobic, non-spore-forming bacterium. Arch Microbiol 133:195–201
    [Google Scholar]
  24. Schnell S., Brune A., Schink B. 1991; Degradation of hydroxyquinone by the strictly anaerobic fermenting bacterium Pelobacter massiliensis sp. nov. Arch Microbiol 155:512–516
    [Google Scholar]
  25. Szewzyk U., Schink B. 1989; Degradation of hydroquinone, gentisate, and benzoate by a fermenting bacterium in pure or defined mixed culture. Arch Microbiol 151:541–545
    [Google Scholar]
  26. Taylor M. M. 1972; Eubacterium fissicatena sp. nov., isolated from the alimentary tract of the goat. J Gen Microbiol 71:457–463
    [Google Scholar]
  27. Van de Peer Y., De Wachter R. 1993; treecon: a software package for the construction and drawing of evolutionary trees. CABIOS 9:177–182
    [Google Scholar]
  28. Widdel F., Pfennig N. 1981; Studies on dissimilatory sulfatereducing bacteria that decompose fatty acids. Isolation of new sulfate reducing bacteria enriched with acetate from saline environments. Description of Desulfobacterpostgatei gen. nov., sp. nov. Arch Microbiol 129:395–400
    [Google Scholar]
  29. Winker S., Woese C. R. 1991; A definition of the domain Archaea, Bacteria and Eucarya in terms of small subunit rRNA characteristics. Syst Appl Microbiol 13:161–165
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-49-4-1741
Loading
/content/journal/ijsem/10.1099/00207713-49-4-1741
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error