1887

Abstract

Two aerobic, Gram-positive bacteria, strains HKI 0122 and HKI 0132, were isolated from a cave. Cells are not acid-fast, non-motile, non-spore-forming and exhibit a rod-coccus growth cycle. The cell wall peptidoglycan contains lysine in position 3 of the peptide subunit and an interpeptide bridge of -Lys ← -Glu. The major menaquinone is MK-8(H), 13-methyl and 12-methyl tetradecanoic acids are the predominating fatty acids. The polar lipids consist of phosphatidylinositol, diphosphatidylglycerol and three unknown phospholipids. Mycolic acids are absent. The DNA base composition is 71 mol% G+C. Phylogenetic analysis revealed that strain HKI 0122 forms a novel taxon among the families and unassigned genera of the suborder , within the order . On the basis of the genotypic, chemotaxonomic, morphological and physiological characteristics of these two isolates it is proposed to assign strains HKI 0122 and HKI 0132 to a new genus and species for which the name gen. nov., sp. nov. is proposed. The type strain is HKI 0122 (= DSM 12333).

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1999-10-01
2024-12-08
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