1887

Abstract

Two aerobic, Gram-positive bacteria, strains HKI 0122 and HKI 0132, were isolated from a cave. Cells are not acid-fast, non-motile, non-spore-forming and exhibit a rod-coccus growth cycle. The cell wall peptidoglycan contains lysine in position 3 of the peptide subunit and an interpeptide bridge of -Lys ← -Glu. The major menaquinone is MK-8(H), 13-methyl and 12-methyl tetradecanoic acids are the predominating fatty acids. The polar lipids consist of phosphatidylinositol, diphosphatidylglycerol and three unknown phospholipids. Mycolic acids are absent. The DNA base composition is 71 mol% G+C. Phylogenetic analysis revealed that strain HKI 0122 forms a novel taxon among the families and unassigned genera of the suborder , within the order . On the basis of the genotypic, chemotaxonomic, morphological and physiological characteristics of these two isolates it is proposed to assign strains HKI 0122 and HKI 0132 to a new genus and species for which the name gen. nov., sp. nov. is proposed. The type strain is HKI 0122 (= DSM 12333).

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1999-10-01
2022-12-08
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References

  1. Altenburger P., Kämpfer P., Makristathis A., Lubitz W., Busse H.-J. 1996; Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47:39–52
    [Google Scholar]
  2. Collins M. D., Jones D. 1980; Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48:459–470
    [Google Scholar]
  3. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230
    [Google Scholar]
  4. De Soete G. 1983; A least square algorithm for fitting additive trees to proximity data. Psychometrika 48:621–626
    [Google Scholar]
  5. DSMZ 1998 Catalogue of Strains, 6th. Braunschweig: Deutsche Sammlung von Mikroorganismen und Zellkulturen;
    [Google Scholar]
  6. Eppard M., Krumbein W. E., Koch C, Rhiel E., Staley J. T., Stackebrandt E. 1996; Morphological, physiological, and molecular characterization of actinomycetes isolated from dry soil, rocks, and monument surfaces. Arch Microbiol 166:12–22
    [Google Scholar]
  7. Felsenstein J. 1993 phylip (Phylogenetic Inference Package) version 3.5.1. Seattle: Department of Genetics, University of Washington;
    [Google Scholar]
  8. Frank H., Rettenmeier A., Weicker H., Nicholson G. J., Bayer E. 1980; A new gas chromatographic method for determination of amino acid levels in human serum. Clin Chim Acta 105:201–211
    [Google Scholar]
  9. Groth I., Saiz-Jimenez C. 1999; Actinomycetes in hypogean environments. Geomicrobiol J 16:1–8
    [Google Scholar]
  10. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. 1996; Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239
    [Google Scholar]
  11. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K. 1997a; Bogoriella caseilytica gen. nov., sp. nov., a new alkaliphilic actinomycete from a soda lake in Africa. Int J Syst Bacteriol 47:788–794
    [Google Scholar]
  12. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K. 1997b; Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 41:1129–1133
    [Google Scholar]
  13. Groth I., Vettermann R., Schuetze B., Schumann P., Saiz-Jimenez C. 1999; Actinomycetes in Karstic caves of northern Spain (Altamira and Tito Bustillo). J Microbiol Methods 36:115–122
    [Google Scholar]
  14. Jukes T. H., Cantor C. R. 1969 Evolution of protein molecules. Mammalian Protein Metabolism 321–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  15. MacKenzie S. L. 1987; Gas chromatographic analysis of amino acids as the A-heptafluorobutyryl isobutyl esters. J Assoc Off Anal Chern 70:151–160
    [Google Scholar]
  16. Maidak B. L, Larsen N., McGaughey M. J., Overbeek R., Olsen G. J., Woese C. R. 1997; The ribosomal database project. Nucleic Acids Res 25:109–111
    [Google Scholar]
  17. Minnikin D. E., Alshamaony L., Goodfellow M. 1975; Differentiation of Mycobacterium, Nocardia, and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. J Gen Microbiol 88:200–204
    [Google Scholar]
  18. Minnikin D. E., Collins M. D., Goodfellow M. 1979; Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 41:87–95
    [Google Scholar]
  19. Palleroni N. J. 1997; Prokaryotic diversity and the importance of culturing. Antonie Leeuwenhoek 72:3–19
    [Google Scholar]
  20. Rainey F. A. 1998; The camels of the prokaryotic world. Abstracts of the 8th International Symposium on Microbial EcologyHalifax, Canada9-14 August 1998
    [Google Scholar]
  21. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092
    [Google Scholar]
  22. Rheims H., Sproer C., Rainey F. A., Stackebrandt E. 1996; Molecular biological evidence for the occurrence of uncultured members of the actinomycete line of descent in different environments and geographical locations. Microbiology 142:2863–2870
    [Google Scholar]
  23. Rheims H., Schumann P., Rhode M., Stackebrandt E. 1998; Verrucosispora gifhornensis gen. nov., sp. nov., a member of the actinobacterial family Micromonosporaceae. Int J Syst Bacteriol 48:1119–1127
    [Google Scholar]
  24. Schleifer K. H. 1985; Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156
    [Google Scholar]
  25. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  26. Schumann P., Prauser H., Rainey F. A., Stackebrandt E., Hirsch P. 1997; Friedmanniella antárctica gen. nov., sp. nov., an LL-diaminopimelic acid-containing actinomycete from Antarctic sandstone. Int J Syst Bacteriol 41:278–283
    [Google Scholar]
  27. Stackebrandt E., Fowler V. J., Fiedler F., Seiler H. 1983; Taxonomic studies on Arthrobacter nicotianae and related taxa: Description of Arthrobacter uratoxydans sp. nov. and Arthrobacter sulfureus sp. nov. and reclassification of Brevibacterium protophormiae as Arthrobacter protophormiae comb. nov. Syst Appl Microbiol 4:470–486
    [Google Scholar]
  28. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491
    [Google Scholar]
  29. Stead D. E., Sellwood J. E., Wilson J., Viney I. 1992; Evaluation of a commercial microbial identification system based on fatty acid profiles for rapid, accurate identification of plant pathogenic bacteria. J Appl Bacteriol 72:315–321
    [Google Scholar]
  30. Uchida K., Aida K. 1984; An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. J Gen Appl Microbiol 30:131–134
    [Google Scholar]
  31. Waite K., Hugghins K., Battista J. R., Ward-Rainey N., Rainey F. A. 1998; Isolation of members of the Geodermatophilus / Blastococcus group from desert soil exposed to ionizing radiation. Abstracts of the 8th International Symposium on Microbial EcologyHalifax, Canada9-14 August 1998
    [Google Scholar]
  32. Yamada K., Komagata K. 1972; Taxonomic studies on coryneform bacteria. IV. Morphological, cultural, biochemical, and physiological characteristics. J Gen Appl Microbiol 18:399–416
    [Google Scholar]
  33. Yokota A., Takeuchi M., Sakane T., Weiss N. 1993; Proposal of six new species of the genus Aureobacterium and transfer of Flavobacterium esteraromaticum Omelianski to the genus Aureobacterium as Aureobacterium esteraromaticum comb. nov. Int J Syst Bacteriol 43:555–564
    [Google Scholar]
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