1887

Abstract

has unique phenotypic characters within the genus . The 16S rRNA sequence of was determined and its phylogenetic position was defined. This micro-organism is a member of the genus but it is not closely related to , the type species of the genus , and is nearer to and . A PCR-based identification systen using species-specific primers designed on the basis of DNA sequences encoding the 16S rRNA of strains of and is described. A species-specific primer set can distinguish from or closely related species including and species. This species-specific PCR method can be used to identify -like species isolated from human faeces. On the basis of the 16S rRNA sequence divergence from and and the presence of unique phenotypic characters, a new genus, gen. nov., is proposed for , with one species, comb. nov. The type strain of is JCM 9979.

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1999-10-01
2024-11-11
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References

  1. Benno Y., Suzuki K., Suzuki K., Narisawa K., Bruce W. R., Mitsuoka T. 1986; Comparison of the fecal microflora in rural Japanese and urban Canadians. Microbiol Immunol 30:521–532
    [Google Scholar]
  2. Chandrasekaran A., Robertson L. W., Reuning R. H. 1987; Reductive inactivation of digitoxin by Eubacterium lentum cultures. Appl Environ Microbiol 53:901–904
    [Google Scholar]
  3. Eggerth A. H. 1935; The Gram-positive non-spore-bearing anaerobic bacilli of human feces. J Bacteriol 30:277–299
    [Google Scholar]
  4. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791
    [Google Scholar]
  5. Finegold S. M., Sutter V. L. 1978; Fecal flora in different populations with special reference to diet. Am J Clin Nutr 27:1456–1469
    [Google Scholar]
  6. Fresia F., Collins M. D. 1987; Vitamin K composition of anaerobic gut bacteria. FEMS Microbiol Lett 41:175–180
    [Google Scholar]
  7. Haas H., König H. 1988; Coriobacteriumglomerans gen. nov., sp. nov. from the intestinal tract of the red soldier bug. Int J Syst Bacteriol 38:382–384
    [Google Scholar]
  8. Hatta M. 1995; Antitumor mechanisms of Eubacterium lentum and its components. Asian Pac J Allergy Immunol 13:129–137
    [Google Scholar]
  9. Itoh U., Sato M., Tsuchiya H., Namikawa I. 1995; Cellular fatty acids and aldehydes of oral Eubacterium. FEMS Microbiol Lett 126:69–74
    [Google Scholar]
  10. Kageyama A., Benno Y., Nakase T. 1999; Phylogenetic and phenotypic evidence for the transfer of Eubacterium aerofaciens to the genus Collinsella as Collinsella aerofaciens gen. nov., comb. nov. Int J Syst Bacteriol 49:557–565
    [Google Scholar]
  11. Kimura M., Ohta T. 1972; On the stochastic model for estimation of mutation distance between homologous proteins. J Mol Evol 2:87–90
    [Google Scholar]
  12. Moore W. E. C., Holdeman L. V. 1974; Human fecal flora: the normal flora of 20 Japanese-Hawaiians. Appi Microbiol 27:961–979
    [Google Scholar]
  13. Moore W. E. C., Cato E. P., Holdeman L V. 1971; Eubacterium lentum (Eggerth) Prévot 1938: emendation of description and designation of the neotype strain. Int J Syst Bacteriol 21:307–310
    [Google Scholar]
  14. Morinaga S., Sakamoto K., Konishi K. 1988; Antitumor activity and properties of Eubacterium lentum. Jpn J Cancer Res 79:117–124
    [Google Scholar]
  15. Mountfort D. O., Grant W. G., Clarke R., Asher R. A. 1988; Eubacterium callanderi sp. nov. that demethoxylates O-methoxylated aromatic acids to volatile fatty acids. Int J Syst Bacteriol 38:254–258
    [Google Scholar]
  16. Nakazawa F., Hoshino E. 1994; Genetic relationships among Eubacterium species. Int J Syst Bacteriol 44:787–790
    [Google Scholar]
  17. Prévot A. R. 1938; Etudes de systématique bactérienne. III. Invalidité du genre Bacteroides Castellani et Chalmers. Démembrement et reclassification. Ann Inst Pasteur Paris 60:295
    [Google Scholar]
  18. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  19. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  20. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491
    [Google Scholar]
  21. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
    [Google Scholar]
  22. Wade W. G., Downes J., Dymock D., Hiom S. J., Weightman A. J., Dewhirst F. E., Paster B. J., Tzellas N., Coleman B. 1999; The family Coriobacteriaceae·. reclassification of Eubacterium exiguum (Poco et al. 1996) and Peptostreptococcus heliotrinreducens (Lanigan 1976) as Slackia exigua gen. nov., comb, nov. and Slackia heliotrinreducens gen. nov., comb, nov., and Eubacterium lentum (Prevot 1938) as Eggerthella lenta gen. nov., comb. nov. Int J Syst Bacteriol 49:595–600
    [Google Scholar]
  23. Willems A., Collins M. D. 1996; Phylogenetic relationships of the genera Acetobacterium and Eubacterium sensu stricto and reclassification of Eubacterium alactolyticum as Pseudoramibacter alactolyticus gen. nov., comb. nov. Int J Syst Bacteriol 46:1083–1087
    [Google Scholar]
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