Two Gram-positive, non-motile, non-spore-forming, strictly aerobic, pigmented cocci, strains Ben 107 and Ben 108, growing in aggregates were isolated from activated sludge samples by micromanipulation. Both possessed the rare type A3γ’ peptidoglycan. Major menaquinones of strain Ben 107 were MK-9(H) and MK-7(H), and the main cellular fatty acid was 12-methyltetradecanoic acid (ai-C). In strain Ben 108, MK-9(H), MK-9(H) and MK-7(H) were the menaquinones and again the main fatty acid was 12-methyltetradecanoic acid (ai-C). Polar lipids in both strains consisted of phosphatidyl inositol, phosphatidyl glycerol and diphosphatidyl glycerol with two other unidentified glycolipids and phospholipids also present in both. These data, together with the 16S rDNA sequence data, suggest that strain Ben 107 belongs to the genus which presently includes a single recently described species, . Although the taxonomic status of strain Ben 108 is far less certain, on the basis of its 16S rRNA sequence it is also adjudged to be best placed in the genus . The chemotaxonomic characteristics and DNA-DNA hybridization data support the view that Ben 107 and Ben 108 are novel species of the genus . Hence, it is proposed that strain Ben 107 (= ACM 5121) is named as sp. nov. and strain Ben 108 (= ACM 5120) as sp. nov.


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