RT Journal Article SR Electronic(1) A1 Hedegaard, Jakob A1 Steffensen, Søren A. de A. A1 Nørskov-Lauritsen, Niels A1 Mortensen, Kim K. A1 Sperling-Petersen, Hans U.YR 1999 T1 Identification of Enterobacteriaceae by partial sequencing of the gene encoding translation initiation factor 2 JF International Journal of Systematic and Evolutionary Microbiology, VO 49 IS 4 SP 1531 OP 1538 DO https://doi.org/10.1099/00207713-49-4-1531 PB Microbiology Society, SN 1466-5034, AB Nucleotide sequence analysis is increasingly being used to identify bacteria. In this work, a PCR assay based on degenerate primers was used to obtain the partial sequence of infB, the gene encoding translation initiation factor 2 (IF2), in 39 clinical isolates of different Enterobacteriaceae. The partial sequence encodes the GTP-binding domain of IF2. Together with sequences from the literature, a total of 15 species, each represented by one to seven strains, was investigated. Phylogenetic analysis yielded an evolutionary tree which had a topology similar to a tree constructed using available 16S rRNA sequences. It is concluded that the inter-species variation of the infB gene fragment is sufficient for its use in the characterization of strains that have aberrant phenotypic reactions., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-49-4-1531