1887

Abstract

Previous investigations demonstrated three taxonomic groups among 22 clinical isolates of . These studies were expanded to 71 clinical isolates, of which 35 (49%) (group 1) were identical to five ATCC reference strains including the type strain ATCC 19420. Twenty-eight isolates (39%) were group 2, and eight isolates (11%) were group 3. Isolates of groups 2 and 3 were most often associated with post-traumatic or post-surgical wound infections including osteomyelitis, were susceptible to sulfamethoxazole, amikacin, imipenem and the tetracyclines, variably resistan to clarithromycin, and susceptible (group 1), intermediately resistant (group 2) or resistant (group 3) to tobramycin. The three groups were similar by routine biochemical and growth characteristics, but had different mycolic acid dimethoxy-4-coumarinylmethyl ester elution patterns by HPLC and different PCR-restriction enzyme patterns of a 439 bp fragment of the gene. Group 3 isolates differed from group 1 by 18 bp by 16S rRNA sequencing and exhibited < 25% homology by DNA-DNA hybridization, being most closely related to . The 16S rRNA of group 1 and group 2 isolates differed by only 3 bp, but by DNA-DNA hybridization they exhibited only 40% homology. The following names are proposed: sp. nov. for group 2 isolates (type strain ATCC 700504 = M069), sp. nov. for group 3 isolates (type strain ATCC 700010 = M0739) and for group 1 isolates.

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1999-10-01
2022-08-17
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References

  1. Baba T., Kaneda K., Kusunose E., Kusunose M., Yano I. 1988; Molecular species of mycolic acid subclasses in eight strains of Mycobacterium smegmatis. Lipids 23:1132–1138
    [Google Scholar]
  2. Böddinghaus B., Rogall T., Flohr T., Blöcker H., Böttger E. C. 1990; Detection and identification of mycobacteria by amplification of rRNA. J Clin Microbiol 28:1751–1759
    [Google Scholar]
  3. Böttger E. C. 1989; Rapid determination of bacterial ribosomal RNA sequences by direct sequencing of enzymatically amplified DNA. FEMS Microbiol Lett 65:171–176
    [Google Scholar]
  4. Brown B. A., Wallace R. J. Jr, Onyi G. O., De Rosas V., Wallace R. J. III 1992; Activities of four macrolides, including clarithromycin, against Mycobacterium fortuitum, Mycobacterium chelonae, and M. chelonae-kke organisms. Antimicrob Agents Chemother 36:180–184
    [Google Scholar]
  5. Butler W. R., Kilburn J. O. 1990; High-performance liquid chromatography patterns of mycolic acids as criteria for identification of Mycobacterium chelonae, Mycobacterium fortuitum, and Mycobacterium smegmatis. J Clin Microbiol 28:2094–2098
    [Google Scholar]
  6. Butler W. R., Cage G., Desmond E.9 other authors 1996a Standardized method for the HPLC identification of mycobacteria Atlanta GA: Centers for Disease Control and Prevention;
    [Google Scholar]
  7. Butler W. R., Cage G., Desmond E.9 other authors 1996b Development of a uniform procedure for high-performance liquid chromatography (HPLC) analysis of mycobacteria and validation of a database for an automated recognition software. Abstracts of the 96th General Meeting of the American Society for Microbiology abstract U-32 106 Washington, DC: American Society for Microbiology;
    [Google Scholar]
  8. Domenech P., Jimenez M. S., Menendez M. C., Bull T. J., Samper S., Manrique A., Garcia M. J. 1997; Mycobacterium mageritense sp. nov. Int J Syst Bacteriol 47:535–540
    [Google Scholar]
  9. Edwards U., Rogal T., Blöcker H., Emde M., Böttger E. C. 1989; Isolation and direct sequencing of entire genes. Characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Res 17:7843–7853
    [Google Scholar]
  10. Gordon R. E., Smith M. M. 1953; Rapidly growing, acid fast bacteria. J Bacteriol 66:41–48
    [Google Scholar]
  11. Hultman T., Stahl E., Hornes E., Uhlen M. 1989; Direct solid phase sequencing of genomic and plasmid DNA using magnetic bead as support. Nucleic Acids Res 17:4937–4946
    [Google Scholar]
  12. Jost K. C. Jr, Dunbar D., Barth S. S., Headley V. L., Elliott L. B. 1995; Identification of Mycobacterium tuberculosis and M. avium complex directly from smear-positive sputum specimens and BACTEC 12B cultures by high-performance liquid chromatography with fluorescence detection and computer-driven pattern recognition models. J Clin Microbiol 33:1270–1277
    [Google Scholar]
  13. Kasatiya S. S., De Thokoly I., Guertin M. 1974; Mycobactéries atypiques isolées des eaux de surface au Québec. Rev Epidém Méd Soc et Santé Publ 22:171–184
    [Google Scholar]
  14. Kazda J., Müller H. J., Stackebrandt E., Daffe M., Müller K., Pitulle C. 1992; Mycobacterium madagascariense sp. nov. Int J Syst Bacteriol 42:524–528
    [Google Scholar]
  15. Kirschner P., Kiekenbeck M., Meissner D., Wolters J., Böttger E. C. 1992a; Genetic heterogeneity within Mycobacterium fortuitum complex species: genotypic criteria for identification. J Clin Microbiol 30:2772–2775
    [Google Scholar]
  16. Kirschner P., Teske A., Schröder K. H., Kroppenstedt R. M., Böttger E. C. 1992b; Mycobacterium confluentis sp. nov. Int J Syst Bacteriol 42:257–262
    [Google Scholar]
  17. Kirschner P., Meier A., Böttger E. C. 1993a Genotypic identification and detection of mycobacteria - facing novel and uncultured pathogens. Diagnostic Molecular Microbiology. Principles and Applications173–190 Edited by Persing D. H., Smith T. F., Tenover F. C., White T. J. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  18. Kirschner P., Springer B., Vogel U., Meier A., Wrede A., Kiekenbeck M., Bange F. C., Böttger E. C. 1993b; Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2 year experience in a clinical laboratory. J Clin Microbiol 31:2882–2889
    [Google Scholar]
  19. Kubica G. P., Baess I., Gordon R. E.9 other authors 1972; A co-operative numerical analysis of rapidly growing mycobacteria. J Gen Microbiol 73:55–70
    [Google Scholar]
  20. Lehmann K. B., Neumann R. O. 1931 Mycobacterium smegmatis. Bacteriology Especially Determinative Bacteriology755–757 New York: G. E. Stechert & Co; Alfred Hafner
    [Google Scholar]
  21. Levy-Frébault V. V., Portaeis F. 1992; Proposed minimal standards for the genus Mycobacterium and for description of new slowly growing Mycobacterium species. Int J Syst Bacteriol 42:315–323
    [Google Scholar]
  22. Lustgarten S. 1885; The bacillus of syphilis. Lancet 1:609–610
    [Google Scholar]
  23. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G + C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167
    [Google Scholar]
  24. National Committee for Clinical Laboratory Standards 1990 Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically, 2nd. Approved standard M7-A2. Villanova, PA: National Committee for Clinical Laboratory Standards;
    [Google Scholar]
  25. Newton J. A. Jr, Weiss P. J., Bowler W. A., Oldfield E. C. III 1993; Soft-tissue infection due to Mycobacterium smegmatis: report of two cases. Clin Infect Dis 16:531–533
    [Google Scholar]
  26. Pennekamp A., Pfyffer G. E., Wüest J., George C. A., Ruef C. 1997; Mycobacterium smegmatis infection in a healthy woman following a facelift: case report and review of the literature. Ann Plast Surg 39:80–83
    [Google Scholar]
  27. Pitulle C., Dorsch M., Kazda J., Wolters J., Stackebrandt E. 1992; Phylogeny of rapidly growing members of the genus Mycobacterium. Int J Syst Bacteriol 42:337–343
    [Google Scholar]
  28. Plaus W. J., Hermann G. 1991; The surgical management of superficial infections caused by atypical mycobacteria. Surgery 110:99–103
    [Google Scholar]
  29. Richardson A. 1970; Bovine mastitis associated with Mycobacterium smegmatis and an untypeable Mycobacterium. Vet Rec 86:497–498
    [Google Scholar]
  30. Richardson A. 1971; The experimental production of mastitis in sheep by Mycobacterium smegmatis and Mycobacterium fortuitum. Cornell Vet 61:640–646
    [Google Scholar]
  31. Rogall T., Flohr T., Bottger E. C. 1982; Differentiation of Mycobacterium species by direct sequencing of amplified DNA. J Gen Microbiol 136:1915–1920
    [Google Scholar]
  32. Rogall T., Wolters J., Flohr T., Bottger E. C. 1990; Towards a phylogeny and definition of species at the molecular level within the genus Mycobacterium. Int J Syst Bacteriol 40:323–330
    [Google Scholar]
  33. Silcox V. A., Good R. C., Floyd M. M. 1981; Identification of clinically significant Mycobacterium fortuitum complex isolates. J Clin Microbiol 14:686–691
    [Google Scholar]
  34. Sourdis J., Nei M. 1998; Relative efficiencies of the maximum parsimony and distance-matrix methods in obtaining the correct phylogenetic tree. Mol Biol Evol 5:298–311
    [Google Scholar]
  35. Springer B., Bottger E. C., Kirschner P., Wallace R. J. Jr 1995; Phylogeny of the Mycobacterium chelonae-ïikc organism based on partial sequencing of the 16S rRNA gene and proposal of Mycobacterium mucogenicum sp. nov. Int J Syst Bacteriol 45:262–267
    [Google Scholar]
  36. Stahl D. A., Urbance J. W. 1990; The division between fast- and slow-growing species corresponds to natural relationships among the mycobacteria. J Bacteriol 172:116–124
    [Google Scholar]
  37. Steingrube V. A., Brown B. A., Gibson J. L, Wilson R. W., Brown J., Blacklock Z., Jost K., Ulrich R. F., Wallace R. J. Jr 1995a; DNA amplification and restriction endonuclease analysis for differentiation of 12 species and taxa of Nocardia, including recognition of four new taxa within the N. asteroides complex. J Clin Microbiol 33:3096–3101
    [Google Scholar]
  38. Steingrube V. A., Gibson J. L, Brown B. A., Zhang Y., Wilson R. W., Rajagopalan M., Wallace R. J. Jr 1995b; PCR amplification and restriction endonuclease analysis of a 65- kilodalton heat shock protein gene sequence for taxonomic separation of rapidly growing mycobacteria. J Clin Microbiol 33:149–153
    [Google Scholar]
  39. Steingrube V. A., Wilson R. W., Brown B. A., Jost K. C, Blacklock Z., Gibson J. L., Wallace R. J. Jr 1997; Rapid identification of clinically significant species and taxa of aerobic actinomycètes, including Actinomadura, Gordona, Nocardia, Rhodococcus, Streptomyces, and Tsukamurella isolates by DNA amplification and restriction endonuclease analysis. J Clin Microbiol 35:817–822
    [Google Scholar]
  40. Swenson J. M., Wallace R. J. Jr, Silcox V. A., Thornsberry C. 1985; Antimicrobial susceptibility of five subgroups of Mycobacterium fortuitum and Mycobacterium chelonae. Antimicrob Agents Chemother 28:807–811
    [Google Scholar]
  41. Telenti A., Marchesi F., Balz M., Bally F., Böttger E. C., Bodmer T. 1993; Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol 31:175–178
    [Google Scholar]
  42. Tonjum T-, Welty D. B., Jantzen E., Small P. L. 1998; Differentiation of Mycobacterium ulcerans, M. marinum, and M. haemophilum·, mapping of their relationships to M. tuberculosis by fatty acid profile analysis, DNA-DNA hybridization, and 16S rRNA gene sequence analysis. J Clin Microbiol 36:918–925
    [Google Scholar]
  43. Tsukamura M. 1984 Identification of mycobacteria Obu, Aichi, Japan: Mycobacteriosis Research Laboratory of the National Chubu Hospital;
    [Google Scholar]
  44. Valero-Guillen P. L., Martin-Luengo F. 1983; A gas-liquid and thin-layer chromatographic study of Mycobacterium fortuitum. Tubercle 64:283–290
    [Google Scholar]
  45. Valero-Guillén P. L, Martin-Luengo F., Larsson L., Jiménez J. 1987; Demonstration of 2-methyl branched-chain fatty acids in some rapid-growing mycobacteria. FEMS Microbiol Lett 44:303–305
    [Google Scholar]
  46. Vonmoos S., Leuenberger P. H., Beer V., de Haller R. 1986; Infection pleuro-pulmonaire à Mycobacterium smegmatis. Schweiz Med Wochenschr 116:1852–1856
    [Google Scholar]
  47. Wallace R. J. Jr, Dalovisio J. R., Pankey G. A. 1980; Disk diffusion testing of susceptibility of Mycobacterium fortuitum and Mycobacterium chelonei to antibacterial agents. Antimicrob Agents Chemother 16:611–614
    [Google Scholar]
  48. Wallace R. J. Jr, Nash D. R., Tsukamura M., Blacklock Z. M.
  49. Silcox V. A. 1988; Human disease due to Mycobacterium smegmatis. J Infect Dis 158:52–59
    [Google Scholar]
  50. Wallace R. J. Jr, Silcox V. A., Tsukamura M., Brown B. A., Kilburn J. O., Butler W. R., Onyi G. 1993; Clinical significance, biochemical features, and susceptibility patterns of sporadic isolates of the Mycobacterium chelonae-Mke organism. J Clin Microbiol 31:3231–3239
    [Google Scholar]
  51. Wayne L., Kubica G. P. 1986 Mycobacteria. Bergey’s Manual of Systematic Bacteriology 21436–1457 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  52. Wayne L. G., Brenner D. J., Colwell R. R.9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464
    [Google Scholar]
  53. Wilkinson G. T., Kelly W. R., O’Boyle D. 1982; Pyogranulomatous panniculitis in cats due to Mycobacterium smegmatis. Austr Vet J 58:77–78
    [Google Scholar]
  54. Wilson R. W., Steingrube V. A., Brown B. A., Wallace R. J. Jr 1998; Clinical application of PCR-restriction enzyme pattern analysis for rapid identification of aerobic actinomycete isolates. J Clin Microbiol 36:148–152
    [Google Scholar]
  55. Wolinsky E., Rynearson T. K. 1968; Mycobacteria in soil and their relation to disease-associated strains. Am Rev Respir Dis 97:1032–1037
    [Google Scholar]
  56. Yassin A. F., Brzezinka H., Schaal K. P. 1993; Cellular fatty acid methyl ester profiles as a tool in the differentiation of members of the genus Mycobacterium. Zentbl Bakteriol 279:316–329
    [Google Scholar]
  57. Zhang Y., Wallace R. J. Jr, Steingrube V. A., Brown B. A., Nash D. R., Silcox V. A., Tsukamura M. 1992; Isoelectric focusing patterns of β-lactamases in the rapidly growing mycobacteria. Tubercle Lung Dis 73:337–344
    [Google Scholar]
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