1887

Abstract

A soil isolate, which had been assigned to the genus , was shown to have properties consistent with its classification in the genus . An almost complete nucleotide sequence of the 16S rDNA of the strain was determined following cloning and sequencing of the amplified gene. The sequence was aligned with those available for members of the family and related taxa and phylogenetic trees were inferred using three tree-making algorithms. The organism consistently formed a distinct monophyletic clade with the type strain of , but DNA-DNA relatedness data showed that the two strains belonged to distinct genomic species. The organism was also distinguished from the type strains of all validly described species of using a battery of phenotypic properties. The genotypic and phenotypic data show that the strain merits recognition as a new species of the genus . The name proposed for the new species is sp. nov. The type strain is IFO 14333.

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1999-10-01
2024-04-16
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References

  1. Chun J., Goodfellow M. 1995; A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45:240–245
    [Google Scholar]
  2. Chun J., Blackall L L, Kang S.-O., Hah Y. C., Goodfellow M. 1997; A proposal to reclassify Nocardiapinensis Blackall et al. as Skermania piniformis gen. nov., comb. nov. Int J Syst Bacteriol 47:127–131
    [Google Scholar]
  3. Chun J., Seong C.-N., Bae K. S., Lee K.-J., Kang S.-O., Goodfellow M., Hah Y. C. 1998; Nocardiaflavorosea sp. nov. Int J Syst Bacteriol 48:901–905
    [Google Scholar]
  4. Embley T. M., Smida J., Stackebrandt E. 1988; The phylogeny of mycolate-less wall chemotype IV actinomycètes and description of Pseudonocardiaceae fam. nov. Syst Appl Microbiol 11:44–52
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791
    [Google Scholar]
  6. Felsenstein J. 1993 phylip (phylogenetic inference package) version 3.5c. Department of Genetics, University of Washington; Seattle, WA, USA:
    [Google Scholar]
  7. Fitch W. M. 1972; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416
    [Google Scholar]
  8. Fitch W. M., Margoliash E. 1967; Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155:279–284
    [Google Scholar]
  9. Goodfellow M., Brown A., Cia J., Chun J., Collins M. D. 1997; Amycolatopsis japonicum sp. nov., an actinomycète producing (S,S)-N,N’-ethylenediaminedisuccinic acid. Syst Appl Microbiol 20:78–84
    [Google Scholar]
  10. Gordon R. E., Mihm J. M. 1962; Identification of Nocardia caviae nov. comb. Ann N Y Acad Sci 98:628–636
    [Google Scholar]
  11. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H. N. 1974; Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24:54–63
    [Google Scholar]
  12. Holt J. G., Krieg N. R., Sneath P. H. A., Staley J. T., Williams S. T. 1994 Bergey’s Manual of Determinative Bacteriology, 9th. Baltimore: Williams & Wilkins;
    [Google Scholar]
  13. Jukes T. H., Cantor C. R. 1969 Evolution of protein molecules. Mammalian Protein Metabolism 321–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  14. Kafatos F. C., Jones C. W., Efsratiadis A. 1979; Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure. Nucleic Acids Res 7:1541–1545
    [Google Scholar]
  15. Kim S. B., Goodfellow M. 1999; Reclassification of Amycolatopsis rugosa Lechevalier et al. 1986 as Prauserella rugosa gen. nov., comb. nov. Int J Syst Bacteriol 49:507–512
    [Google Scholar]
  16. Lechevalier M. P., Lechevalier H. A. 1970; Chemical composition as a criterion in the classification of aerobic actinomycètes. Int J Syst Bacteriol 20:435–443
    [Google Scholar]
  17. Lechevalier M. P., Prauser H., Labeda D. P., Ruan J. S. 1986; Two new genera of nocardioform actinomycètes: Amycolata gen. nov. and Amycolatopsis gen. nov. Int J Syst Bacteriol 36:29–37
    [Google Scholar]
  18. Lu Y., Yan X. 1983; Studies on the classification of thermophilic actinomycètes IV. Determination of thermophilic members of Nocardiaceae. Acta Microbiol Sin 23:220–228
    [Google Scholar]
  19. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B:195–206
    [Google Scholar]
  20. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. 1980; Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188:221–233
    [Google Scholar]
  21. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241
    [Google Scholar]
  22. Mordarska H., Mordarski M., Goodfellow M. 1972; Chemo-taxonomic characters and classification of some nocardioform bacteria. J Gen Microbiol 71:77–86
    [Google Scholar]
  23. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for constructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  24. Sasser M. 1980 Identification of bacteria by gas chromatography of cellular fatty acids. Technical Note 101. Newark, DE: MIDI;
    [Google Scholar]
  25. Skerman V. B. D., McGowan V., Sneath P. H. A. 1980; Approved lists of bacterial names. Int J Syst Bacteriol 30:225–420
    [Google Scholar]
  26. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  27. Warwick S., Bowen T., McVeigh H., Embley T. M. 1994; A phylogenetic analysis of the family Pseudonocardiaceae and the genera Actinokineospora and Saccharothrix with 16S rRNA sequences and a proposal to combine the genera Amycolata and Pseudonocardia in the emended genus Pseudonocardia. Int J Syst Bacteriol 44:293–299
    [Google Scholar]
  28. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. 1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813
    [Google Scholar]
  29. Williams S. T., Sharpe M. E., Holt J. G. 1989 Bergey’s Manual of Systematic Bacteriology 4 Baltimore: Williams & Wilkins;
    [Google Scholar]
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