1887

Abstract

SDS-PAGE of total bacterial proteins was applied to the classification of 25 Sudanese and five Kenyan strains isolated from the root nodules of and . Twenty strains were also studied by multilocus enzyme electrophoresis (MLEE) and the whole 16S rRNA gene was sequenced from two strains representing the two major clusters. These results, together with the previously reported numerical taxonomy analysis, pulsed-field gel electrophoresis studies, DNA-DNA dot-blot hybridization, genomic fingerprinting using repetitive sequence-based PCR, DNA base composition analysis, DNA-DNA reassociation analysis, partial sequencing of the 16S rRNA gene and RFLP analysis of the amplified 16S rRNA gene, showed that all 30 strains belong to the genus . Two of the strains grouped with and seven with , while the rest did not cluster with any of the established species. The majority of the strains formed two phenotypically and genotypically distinct groups and we therefore propose that these strains should be classified as two new species, sp. nov. and sp. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-49-4-1359
1999-10-01
2023-01-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/49/4/ijs-49-4-1359.html?itemId=/content/journal/ijsem/10.1099/00207713-49-4-1359&mimeType=html&fmt=ahah

References

  1. Amarger N., Macheret V., Laguerre G. 1997; Rhizobium gallicum sp. nov. and Rhizobium giardinii sp. nov., from Phaseolus vulgaris nodules. Int J Syst Bacteriol 47:996–1006
    [Google Scholar]
  2. van Berkum P., Beyene D., Bao G., Campbell T. A., Eardly B. D. 1998; Rhizobium mongolense sp. nov. is one of three rhizobial genotypes identified which nodulate and form nitrogen-fixing symbioses with Medicago ruthenica [(L.) Ledebour]. Int J Syst Bacteriol 48:13–22
    [Google Scholar]
  3. Chen W. X., Li G. S., Qi Y. L, Wang E. T., Yuan H. L., Li J. L. 1991; Rhizobium huakuii sp. nov. isolated from the root nodules of Astragalus sinicus. Int J Syst Bacteriol 41:275–280
    [Google Scholar]
  4. Chen W. X., Wang E. T., Wang S., Li Y., Chen X., Li Y. 1995; Characteristics of Rhizobium tianshanense sp. nov., a moderately and slowly growing root nodule bacterium isolated from an arid saline environment in Xinjiang, People’s Republic of China. Int J Syst Bacteriol 45:153–159
    [Google Scholar]
  5. Chen W.-X., Tan Z.-Y., Gao J.-L, Li Y., Wang E.-T. 1997; Rhizobium hainanense sp. nov., isolated from tropical legumes. Int J Syst Bacteriol 47:870–873
    [Google Scholar]
  6. Dreyfus B., Garcia J. L., Gillis M. 1988; Characterization of Azorhizobium caulinodans gen. nov., sp. nov., a stem-nodulating nitrogen-fixing bacterium isolated from Sesbania rostrata. Int J Syst Bacteriol 38:89–98
    [Google Scholar]
  7. Eardly B. D. 1994 Multilocus enzyme electrophoresis methods for the analysis of bacterial population genetic structure. Methods of Soil Analysis, Part 2. Microbiological and Biochemical Properties557–573 Edited by Weaver R. W., Angle J. S., Bottomley P. J. Madison, WI: Soil Science Society of America;
    [Google Scholar]
  8. Eardly B. D., Materon L. A., Smith N. H., Johnson D. A., Rumbaugh M. D., Selander R. K. 1990; Genetic structure of natural populations of the nitrogen-fixing bacterium Rhizobium meliloti. Appl Environ Microbiol 56:187–194
    [Google Scholar]
  9. Edwards U., Rogall T., Blöcker H., Emde M., Böttger E. C. 1989; Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Res 17:7843–7853
    [Google Scholar]
  10. Haukka K., Lindstrom K. 1994; Pulsed-field gel electrophoresis for genotypic comparison of Rhizobium bacteria that nodulate leguminous trees. FEMS Microbiol Lett 119:215–220
    [Google Scholar]
  11. Haukka K., Lindstrom K., Young J. P. W. 1996; Diversity of partial 16S rRNA sequences among and within strains of African rhizobia isolated from Acacia and Prosopis. Syst Appl Microbiol 19:352–359
    [Google Scholar]
  12. Hultman T., Bergh S., Moks T., Uhlen M. 1991; Bidirectional solid-phase sequencing of in vitro-amplified plasmid DNA. BioTechniques 10:84–93
    [Google Scholar]
  13. Jarvis B. D. W., Pankhurst C. E., Patel J. J. 1982; Rhizobium loti, a new species of legume root nodule bacteria. Int J Syst Bacteriol 32:378–380
    [Google Scholar]
  14. Jarvis B. D. W., van Berkum P., Chen W. X., Nour S. M., Fernandez M. P., Cleyet-Marel J. C., Gillis M. 1997; Transfer of Rhizobium loti, Rhizobium huakuii, Rhizobium ciceri, Rhizobium mediterraneum, and Rhizobium tianshanense to Mesorhizobium gen. nov. Int J Syst Bacteriol 47:895–898
    [Google Scholar]
  15. Jordan D. C. 1984 Family III. Rhizobiaceae Conn 1938. Bergey’s Manual of Systematic Bacteriology 1234–256 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  16. Kersters K., De Ley J. 1984 Genus III. Agrobacterium Conn 1942. Bergey’s Manual of Systematic Bacteriology 1244–254 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  17. Kiredjian M., Holmes B., Kersters K., Guilvout I., De Ley J. 1986; Alcaligenespiechaudii, a new species from human clinical specimens and the environment. Int J Syst Bacteriol 36:282–287
    [Google Scholar]
  18. Kuykendall L. D., Saxena B., Devine T. E., Udell S. E. 1992; Genetic diversity in Bradyrhizobium japonicum Jordan 1982 and a proposal for Bradyrhizobium elkanii sp. nov. Can J Microbiol 38:501–505
    [Google Scholar]
  19. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  20. de Lajudie P., Willems A., Pot B.7 other authors 1994; Polyphasic taxonomy of rhizobia: emendation of the genus Sinorhizobium and description of Sinorhizobium meliloti comb, nov., Sinorhizobium saheli sp. nov., and Sinorhizobium teranga sp. nov. Int J Syst Bacteriol 44:715–733
    [Google Scholar]
  21. de Lajudie P., Willems A., Nick G.9 other authors 1998; Characterization of tropical tree rhizobia and description of Mesorhizobium plurifarium sp. nov. Int J Syst Bacteriol 48:369–382
    [Google Scholar]
  22. Lindstrom K. 1989; Rhizobium galegae, a new species of legume root nodule bacteria. Int J Syst Bacteriol 39:365–367
    [Google Scholar]
  23. Lindstrom K., Sarsa M., Polkunen J., Kansanen P. 1985; Symbiotic nitrogen fixation of Rhizobium (Galega) in acid soils and its survival in soil under acid and cold stress. Plant Soil 87:293–302
    [Google Scholar]
  24. Martinez-Romero E., Segovia L., Mercante F. M., Franco A. A., Graham P., Pardo M. A. 1991; Rhizobium tropici, a novel species nodulating Phaseolus vulgaris L. beans and Leucaena sp. trees. Int J Syst Bacteriol 41:417–426
    [Google Scholar]
  25. Nick G. 1998 Polyphasic taxonomy of rhizobia isolated from tropical tree legumes PhD thesis Dissertationes Biocentri Viikki Universitatis Helsingiensis, Department of Applied Chemistry and Microbiology; University of Helsinki:
    [Google Scholar]
  26. Nick G., Jussila M., Hoste B., Niemi M., Kaijalainen S., de Lajudie P., Gillis M., de Bruijn F. J., Lindstrom K. 1999; Rhizobia isolated from the root nodules of tropical leguminous trees characterized using DNA-DNA dot-blot hybridisation and rep-PCR. Syst Appl Microbiol in press
    [Google Scholar]
  27. Nour S. M., Fernandez M. P., Normand P., Cleyet-Marel J.-C. 1994; Rhizobium ciceri sp. nov., consisting of strains that nodulate chickpeas (Cicer arietinum L.). Int J Syst Bacteriol 44:511–522
    [Google Scholar]
  28. Nour S. M., Cleyet-Marel J.-C., Normand P., Fernandez M. P. 1995; Genomic heterogeneity of strains nodulating chickpeas (Cicer arietinum L.) and description of Rhizobium mediterraneum sp. nov. Int J Syst Bacteriol 45:640–648
    [Google Scholar]
  29. Ophel K., Kerr A. 1990; Agrobacterium vitis sp. nov. for strains of Agrobacterium biovar 3 from grapevines. Int J Syst Bacteriol 40:236–241
    [Google Scholar]
  30. Page R. D. M. 1996; TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358
    [Google Scholar]
  31. Pot B., Gillis M., Hoste B., Van De Velde A., Bekaert F., Kersters K., De Ley J. 1989; Intra- and intergeneric relationships of the genus Oceanospirilium. Int J Syst Bacteriol 39:23–34
    [Google Scholar]
  32. Pot B., Vandamme P., Kersters K. 1994 Analysis of electrophoretic whole organism protein fingerprints. Chemical Methods in Prokaryotic Systematics493–521 Edited by Goodfellow M., O’Donnell T. Chichester: Wiley;
    [Google Scholar]
  33. Ridell J., Siitonen A., Paulin L., Lindroos O., Korkeala H., Albert M. J. 1995; Characterization of Hafnia alvei by biochemical tests, random amplified polymorphic DNA PCR, and partial sequencing of 16S rRNA gene. J Clin Microbiol 33:2372–2376
    [Google Scholar]
  34. Rome S., Fernandez M. P., Brunel B., Normand P., Cleyet-Marel J.-C. 1996; Sinorhizobium medicae sp. nov., isolated from annual Medicago spp. Int J Syst Bacteriol 46:972–980
    [Google Scholar]
  35. Scholia M. H., Elkan G. H. 1984; Rhizobium fredii sp. nov., a fast-growing species that effectively nodulates soybeans. Int J Syst Bacteriol 34:484–486
    [Google Scholar]
  36. Segovia L., Young J. P. W., Martinez-Romero E. 1993; Reclassification of American Rhizobium leguminosarum biovar phaseoli type I strains as Rhizobium etli sp. nov. Int J Syst Bacteriol 43:374–377
    [Google Scholar]
  37. Selander R. K., Caugant D. A., Ochman H., Musser J. M., Gilmour M. N., Whittam T. S. 1986; Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Appl Environ Microbiol 51:873–884
    [Google Scholar]
  38. Sneath P. H. A., Sokal R. R. 1973 Numerical Taxonomy San Francisco: W. H. Freeman;
    [Google Scholar]
  39. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
    [Google Scholar]
  40. Vauterin L., Vauterin V. 1992; Computer-aided objective comparison of electrophoresis patterns for grouping and identification of microorganisms. Eur Microbiol 1:37–41
    [Google Scholar]
  41. Wang E. T., van Berkum P., Beyene D., Sui X. H., Dorado O., Chen W. X., Martinez-Romero E. 1998; Rhizobium huautlense sp. nov., a symbiont of Sesbania herbacea that has a close phylogenetic relationship with Rhizobium galegae. Int J Syst Bacteriol 48:687–699
    [Google Scholar]
  42. Xu L. M., Ge C., Cui Z., Li J., Fan H. 1995; Bradyrhizobium liaoningense sp. nov., isolated from the root nodules of soybeans. Int J Syst Bacteriol 45:706–711
    [Google Scholar]
  43. Zhang X., Harper R., Karsisto M., Lindstrom K. 1991; Diversity of Rhizobium bacteria isolated from the root nodules of leguminous trees. Int J Syst Bacteriol 41:104–113
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-49-4-1359
Loading
/content/journal/ijsem/10.1099/00207713-49-4-1359
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error