1887

Abstract

Isolates from the complex cultured from caprine pathological tissue samples were biochemically and genetically characterized. The isolates were negative for nitrate reduction and niacin accumulation, they weakly hydrolysed Tween 80, were sensitive to pyrazinamide (50 μg ml) and were resistant to 1 and 2 μg tiophene-2-carboxylic acid hydrazide ml but not to 5 or 10 μg tiophene-2-carboxylic acid hydrazide ml. Sequencing of the gene revealed a polymorphism characteristic of , whereas and sequences were characteristic of The fingerprinting patterns obtained with IS6110, direct repeats and polymorphic G+C-rich sequence-associated RFLP and direct variable repeat-spacer oligonucelotide typing (spoligotyping) segregated these isolates from the other members of the complex. The results of this testing, together with the repeated association of this micro-organism with goats, suggest that a new member of this taxonomic complex not matching any of the classical species had been identified. This unusual mycobacterium may play a role in the epidemiology of animal and human tuberculosis in Spain. The name subsp. subsp. nov. is proposed for these isolates. The type strain of subsp. subsp. nov. is gM-1 (= CIP 105776).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-49-3-1263
1999-07-01
2022-05-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/49/3/ijs-49-3-1263.html?itemId=/content/journal/ijsem/10.1099/00207713-49-3-1263&mimeType=html&fmt=ahah

References

  1. Alfredsen S., Saxegaard F. 1992; An outbreak of tuberculosis in pigs and cattle caused by Mycobacterium africanum. Vet Rec 131:51–53
    [Google Scholar]
  2. Anonymous 1954; Towards a standardization of laboratory methods. Bull Int Union Tuberc 25:89–95
    [Google Scholar]
  3. Aranaz A., Liebana E., Mateos A. 8 other authors 1996a; Spacer oligonucleotide typing of Mycobacterium bovis strains from cattle and other animals: a tool for studying the epidemiology of tuberculosis. J Clin Microbiol 34:2734–2740
    [Google Scholar]
  4. Aranaz A., Liebana E., Pickering X., Novoa C., Mateos A., Dominguez L. 1996b; Use of polymerase chain reaction in the diagnosis of tuberculosis in cats and dogs. Vet Rec 138:276–280
    [Google Scholar]
  5. Aranaz A., Liebana E., Mateos A., Dormnguez L., Cousins D. V. 1998; Restriction fragment length polymorphism and spacer oligonucleotide typing: a comparative analysis of fingerprinting strategies for Mycobacterium bovis. Vet Microbiol 61:311–324
    [Google Scholar]
  6. Baess I. 1979; Deoxyribonucleic acid relatedness among species of slowly-growing mycobacteria. Acta Pathol Microbiol Scand Sect B 87:221–226
    [Google Scholar]
  7. Blazquez J., Espinosa de los Monteros L E., Samper S., Martfn C., Guerrero A., Cobo J., van Embden J., Baquero F., Gemez- Mampaso E. 1997; Genetic characterization of multidrug- resistant Mycobacterium bovis strains from a hospital outbreak involving human immunodeficiency virus-positive patients. J Clin Microbiol 35:1390–1393
    [Google Scholar]
  8. Boddinghaus B., Rogall T., Floht T., Blficker H., Bottger E. C. 1990; Detection and identification of mycobacteria by amplification of rRNA. J Clin Microbiol 28:1751–1759
    [Google Scholar]
  9. Bradley S. G. 1973; Relationships among mycobacteria and nocardiae based upon deoxyribonucleic acid reassociation. J Bacteriol 113:645–651
    [Google Scholar]
  10. Castets M., Rist N., Boisvert H. 1969; La variete africaine du bacille tuberculeux humain. Med Afr Noire 16:321–322
    [Google Scholar]
  11. Clercx G., Coignoul F., Jakovljevic S., Balligand M., Mainil J., Henroteaux M., Kaeckenbeeck A. 1992; Tuberculosis in dogs: a case report and review of the literature. J Am Anim Hosp Assoc 28:207–211
    [Google Scholar]
  12. Coletsos P. J. 1971; Isolation des mycobacteries. Rev Tuberc Pneumol (Paris) 35:601–616
    [Google Scholar]
  13. Collins C. H., Yates M. D. 1982; Subdivision of Mycobacterium tuberculosis into five variants for epidemiological purposes: methods and nomenclature. J Hyg 89:235–242
    [Google Scholar]
  14. Collins C. H., Grange J. M. 1983; The bovine tubercle bacillus. J Appl Bacteriol 55:13–29
    [Google Scholar]
  15. Corner L. A., Trajstman A. C. 1988; An evaluation of 1- hexadecyl-pyridinium chloride as a decontaminant in the primary isolation of Mycobacterium bovis from bovine lesions. Vet Microbiol 18:127–134
    [Google Scholar]
  16. Cousins D. V., Williams S. W., Ross B. C., Ellis T. M. 1993; Use of a repetitive element isolated from Mycobacterium tuberculosis in hybridisation studies with Mycobacterium bovis: a new tool for epidemiological studies of bovine tuberculosis. Vet Microbiol 37:1–17
    [Google Scholar]
  17. Cousins D., Williams S., Liebana E., Aranaz A., Bunschoten A., van Embden J., Ellis T. 1998; Evaluation of four DNA typing techniques in epidemiological investigations of bovine tuberculosis. J Clin Microbiol 36:168–178
    [Google Scholar]
  18. Espinosa de los Monteros L. E., Galan J. C., Gutierrez M. 8 other authors 1998; Allele-specific method based on pncA and oxyR sequences for distinguishing Mycobacterum bovis from Mycobacterium tuberculosis’, intraspecific M. bovis pncA sequence polymorphism. J Clin Microbiol 36:239–242
    [Google Scholar]
  19. Fiss E. H., Chehab F. F., Brooks G. F. 1992; DNA amplification and reverse dot blot hybridization for detection and identification of mycobacteria to the species level in the clinical laboratory. J Clin Microbiol 30:1220–1224
    [Google Scholar]
  20. Frothingham R., Hills H. G., Wilson K. H. 1994; Extensive DNA sequence conservation throughout the Mycobacterium tuberculosis complex. J Clin Microbiol 32:1639–1643
    [Google Scholar]
  21. Glennon M., Smith T., Cormican M., Noone D., Barry T., Maher M., Dawson M., Gilmartin J. J., Gannon F. 1994; The ribosomal intergenic spacer region: a target for the PCR based diagnosis of tuberculosis. Tubercle Lung Dis 75:353–360
    [Google Scholar]
  22. Goguet de la Salmoniere Y. O., Minh Li H., Torrea G., Bunschoten A., van Embden J., Gicquel B. 1997; Evaluation of spoligotyping in a study of the transmission of Mycobacterium tuberculosis. J Clin Microbiol 35:2210–2214
    [Google Scholar]
  23. Gutierrez M., Samper S., Jimenez M. S., van Embden J. D. A., Garcia J. F., Martrn C. 1997; Identification by spoligotyping of a caprine genotype in Mycobacterium bovis strains causing human tuberculosis. J Clin Microbiol 35:3328–3330
    [Google Scholar]
  24. Hermans P. W. M., van Soolingen D., Dale J. W., Schuitema A. R. J., McAdam R. A., Catty D., van Embden J. D. A. 1990; Insertion element IS956 from Mycobacterium tuberculosis’, a useful tool for the diagnosis and epidemiology of tuberculosis. J Clin Microbiol 28:2051–2058
    [Google Scholar]
  25. Heym B., Alzari P. M., Honore N., Cole S. T. 1995; Missense mutations in the catalase-peroxidase gene, katG, are associated with isoniazid resistance in Mycobacterium tuberculosis. Mol Microbiol 15:235–245
    [Google Scholar]
  26. Hoop R. K., Bottger E. C., Pfyffer G. E. 1996; Etiological agents of mycobacterioses in pet birds between 1986 and 1995. J Clin Microbiol 34:991–992
    [Google Scholar]
  27. Huitema H., Jaartsveld F. H. J. 1967; Mycobacterium microti infection in a cat and some pigs. Antonie Leeuwenhoek 33:209–212
    [Google Scholar]
  28. Imaeda T. 1985; Deoxyribonucleic acid relatedness among selected strains of Mycobacterium tuberculosis, Mycobacterium bovis, Mycobacterium bovis BCG, Mycobacterium microti and Mycobacterium africanum. Int J Syst Bacteriol 35:147–150
    [Google Scholar]
  29. Kamerbeek J., Schouls, L, Kolk A. 8 other authors 1997; Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology. J Clin Microbiol 35:907–914
    [Google Scholar]
  30. Karlson A. G., Lessel E. F. 1970; Mycobacterium bovis nom. nov. Int J Syst Bacteriol 20:273–282
    [Google Scholar]
  31. Karlson A. G., Martin J. K., Harrington R. 1964; Identification of Mycobacterium tuberculosis with one tube of liquid medium. Mayo Clin Proc 39:410–415
    [Google Scholar]
  32. Kent P. T., Kubica G. P. 1985; Antituberculous chemotherapy and drug susceptibility testing. In Public Health Mycobacteriology: a Guide for the Level III Laboratory pp. 159–184 Atlanta: US Department of Health and Human Services, Centers for Disease Control;
    [Google Scholar]
  33. Kleeberg H. H., Nel E. E. 1969; Porcine mycobacterial lymphadenitis. J S Afr Vet Med Assoc 40:233–250
    [Google Scholar]
  34. Levy-Frebault V. V., Portaels F. 1992; Proposed minimal standards for the genus Mycobacterium and for description of new slowly growing Mycobacterium species. Int J Syst Bacteriol 42:315–323
    [Google Scholar]
  35. Liebana E., Aranaz A., Francis B., Cousins D. V. 1996; Assessment of genetic markers for species differentiation within the Mycobacterium tuberculosis complex. J Clin Microbiol 34:933–938
    [Google Scholar]
  36. Liebana E., Aranaz A., Gonzalez O., Domingo M., Vidal D., Mateos A., Rodriguez-Ferri E. F., Dominguez L., Cousins D. V. 1997; The insertion element IS6II0 is a useful tool for DNA fingerprinting of Mycobacterium bovis isolates from cattle and goats in Spain. Vet Microbiol 54:223–233
    [Google Scholar]
  37. Liu S. K., Weitzman I., Johnson G. G. 1980; Canine tuberculosis. J Am Vet Med Assoc 177:164–167
    [Google Scholar]
  38. Luquin M., Ausina V., Lopez-Calahorra F., Belda F., Garcfa- Barcelo M., Celma C., Prats G. 1991; Evaluation of practical chromatographic procedures for identification of clinical isolates of mycobacteria. J Clin Microbiol 29:120–130
    [Google Scholar]
  39. Lutz B. 1992; My cobacteriology. Identification tests for mycobacteria. In Clinical Microbiological Procedures Handbook pp. 3-12–1-13 Edited by Eisemberg H. D. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  40. Michalak K., Austin C., Diesel S., Bacon J. M., Zimmerman P., Maslow J. N. 1998; Mycobacterium tuberculosis infection as a zoonotic disease: transmission between humans and elephants. Emerg Infect Dis 4283–287
    [Google Scholar]
  41. Musser J. M., Kapur V., Williams D. L., Kreiswirth B. N., van Soolingen D., van Embden J. D. A. 1996; Characterization of the catalase-peroxidase gene {katG) and inhA locus in isoniazid- resistant and -susceptible strains of Mycobacterium tuberculosis by automated DNA sequencing: restricted array of mutations associated with drug resistance. J Infect Dis 173:196–202
    [Google Scholar]
  42. O’Reilly L. M., Daborn C. J. 1995; The epidemiology of Mycobacterium bovis infections in animals and man: a review. Tubercle Lung Dis 76:(Suppl. 1)1–6
    [Google Scholar]
  43. Pattyn S. R., Portaels F. A., Kageruka P., Gigase P. 1970; Mycobacterium microti infection in vicuna (Lama vicugna). Acta Zool Pathol Antverp 51:17–24
    [Google Scholar]
  44. Plikaytis B. B., Plikaytis B. D., Yakrus M. A., Butler W. R., Woodley C. L., Silcox V. A., Shinnick T. M. 1992; Differentiation of slowly growing Mycobacterium species, including Mycobacterium tuberculosis, by gene amplification and restriction fragment length polymorphism analysis. J Clin Microbiol 30:1815–1822
    [Google Scholar]
  45. Reed G. B. 1957; Genus Mycobacterium (species affecting warm-blooded animals except those causing leprosy). In Bergey’s Manual of Determinative Bacteriology vol. 2 section 16, p. 1443 Edited by Breed R. S., Murray E. G. D., Smith N. R. Baltimore: Williams Wilkins;
    [Google Scholar]
  46. Rogall T., Flohr T., Bottger E. C. 1990; Differentiation of Mycobacterium species by direct sequencing of amplified DNA. J Gen Microbiol 136:1915–1920
    [Google Scholar]
  47. Scorpio A., Zhang Y. 1996; Mutations in pncA, a gene encoding pyrazinamidase/ nicotinamidase, cause resistance to the antituberculous drug pyrazinamide in tubercle bacillus. Nat Med 6662–667
    [Google Scholar]
  48. Scorpio A., Collins D., Whipple D., Cave D., Bates J., Zhang Y. 1997; Rapid differentiation of bovine and human tubercle bacilli based on a characteristic mutation in the bovine pyrazinamidase gene. J Clin Microbiol 35:106–110
    [Google Scholar]
  49. Sherman D. R., Sabo P. J., Hickey M. J., Arain T. M., Mahairas G. M., Yuan Y., III Barry C. E., Stover K. 1995; Disparate responses to oxidative stress in saprophytic and pathogenic bacteria. Proc Natl Acad Sci USA 92:6625–6629
    [Google Scholar]
  50. Snider W. R. 1971; Tuberculosis in canine and feline populations. Review of the literature. Am Rev Respir Dis 104:877–887
    [Google Scholar]
  51. Sola C., Horgen L., Maisetti J., Devallois A., Goh K. S., Rastogi N. 1998; Spoligotyping followed by double-repetitive- element PCR as rapid alternative to IS<5770 fingerprinting for epidemiological studies of tuberculosis. J Clin Microbiol 36:1122–1124
    [Google Scholar]
  52. van Soolingen D., Hoogenboezem T., de Haas P. E. W. 9 other authors 1997; A novel pathogenic taxon of the Mycobacterium tuberculosis complex, Canetti: characterization of an exceptional isolate from Africa. Int J Syst Bacteriol 47:1236–1245
    [Google Scholar]
  53. Sreevatsan S., Escalante P., Pan X. 11 other authors 1996; Identification of a polymorphic nucleotide in oxyR specific for Mycobacterium bovis. J Clin Microbiol 34:2007–2010
    [Google Scholar]
  54. Sreevatsan S., Pan X., Stockbauer K. E., Connell N. D., Kreiswirth B., Whittam T. S., Musser J. M. 1997; Restricted structural gene polymorphism in the Mycobacterium tuberculosis complex indicates evolutionarily recent global dissemination. Proc Natl Acad Sci USA 94:9869–9874
    [Google Scholar]
  55. Stackebrandt E., Woese C. R. 1981; The evolution of prokaryotes. In Molecular and Cellular Aspects of Microbial Evolution pp. 1–31 Edited by Carlile M. C., Collins J. F., Moseley B. E. B. Cambridge: Cambridge University Press;
    [Google Scholar]
  56. Tacquet A., Tison F. 1961; Nouvelle technique d’isolement des mycobacteries par le laurylsulfate de sodium. Ann Inst Pasteur 100:676–680
    [Google Scholar]
  57. Takewaki S.-L., Okuzumi K., Ishiko H., Nakahara K.-L., Ohkubo A., Nagai R. 1993; Genus-specific polymerase chain reaction for the mycobacterial dnaJ gene and species-specific oligonucleotide probes. J Clin Microbiol 31:446–450
    [Google Scholar]
  58. Takiff H. E., Salazar, L, Guerrero C., Philipp W., Huang W. M., Kreiswirth B., Cole S. T., Jacobs W. R., Telenti A. 1994; Cloning and nucleotide sequence of Mycobacterium tuberculosis gyrA and gyrB genes and detecton of quinolone resistance mutations. Antimicrob Agents Chemother 38:773–780
    [Google Scholar]
  59. Telenti A., Marchesi F., Balz M., Bally F., Bottger E. C., Bodmer T. 1993; Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol 31:175–178
    [Google Scholar]
  60. Thoen C. O. 1994; Tuberculosis in wild and domestic mammals. In Tuberculosis: Pathogenesis, Protection and Control pp. 157–162 Edited by Bloom B. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  61. Thorel M. F. 1980; Isolation of Mycobacterium africanum from monkeys. Tubercle 61:101–104
    [Google Scholar]
  62. Tsukamura M. 1976; Numerical classification of slowly growing mycobacteria. Int J Syst Bacteriol 26:409–420
    [Google Scholar]
  63. Uhl J. R., Sandhu G. S., Kline B. C., Cockerill F. R. III 1996; PCR-RFLP detection of point mutations in the catalase- peroxidase gene (katG) of Mycobacterium tuberculosis associated with isoniazid resistance. In PCR Protocols for Emerging Infectious Diseases pp. 144–149 Edited by Persing D. H. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  64. Wells A. Q., Oxon D. M. 1937; Tuberculosis in wild voles. Lancet1221
    [Google Scholar]
  65. Wilton S., Cousins D. 1992; Detection and identification of multiple mycobacterial pathogens by DNA amplification in a single tube. PCR Methods Appl 1:269–273
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-49-3-1263
Loading
/content/journal/ijsem/10.1099/00207713-49-3-1263
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error