Isolates from the complex cultured from caprine pathological tissue samples were biochemically and genetically characterized. The isolates were negative for nitrate reduction and niacin accumulation, they weakly hydrolysed Tween 80, were sensitive to pyrazinamide (50 μg ml) and were resistant to 1 and 2 μg tiophene-2-carboxylic acid hydrazide ml but not to 5 or 10 μg tiophene-2-carboxylic acid hydrazide ml. Sequencing of the gene revealed a polymorphism characteristic of , whereas and sequences were characteristic of The fingerprinting patterns obtained with IS6110, direct repeats and polymorphic G+C-rich sequence-associated RFLP and direct variable repeat-spacer oligonucelotide typing (spoligotyping) segregated these isolates from the other members of the complex. The results of this testing, together with the repeated association of this micro-organism with goats, suggest that a new member of this taxonomic complex not matching any of the classical species had been identified. This unusual mycobacterium may play a role in the epidemiology of animal and human tuberculosis in Spain. The name subsp. subsp. nov. is proposed for these isolates. The type strain of subsp. subsp. nov. is gM-1 (= CIP 105776).


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