1887

Abstract

Earlier phylogenetic studies based on the inferred DNA sequences of the and genes suggested that strains of the species formed two clusters, indicating the presence two closely related taxa; one contained the laboratory strain 168 and the other the laboratory strain W23. Significant sexual isolation was found between strain 168 and members of the group containing W23, but no sexual isolation was observed between strain 168 and other members of the 168 group. DNA reassociation between the two groups ranged from 58 to 69% and intragroup DNA relatedness ranged from 82 to 100%. Because group 168 strains were highly related to the type strain, they were considered to be bona fide members of the species. About 99.5% sequence identity was observed between the 16S rRNA genes of the 168 and W23 groups. Ribitol and anhydroribitol were principal cell wall constituents of the W23 but not of the 168 group. These observations revealed two closely related but genetically and phenotypically distinct groups within that correspond to two historically important strains. Subspecies distinction is proposed for the 168 and W23 groups, with the names subsp. subsp. nov. and subsp. subsp. nov., respectively. The type strain of the former is NRRL NRS-744 and the latte NRRL B-23049.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-49-3-1211
1999-07-01
2024-12-12
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/49/3/ijs-49-3-1211.html?itemId=/content/journal/ijsem/10.1099/00207713-49-3-1211&mimeType=html&fmt=ahah

References

  1. Burger M. M., Glaser L. 1964; The synthesis of teichoic acids. I. Polyglycerophosphates. J Biol Chem 239:3168–3177
    [Google Scholar]
  2. Chin T., Burger M. M., Glaser L. 1966; Synthesis of teichoic acids. VI. The formation of multiple wall polymers in Bacillus subtilis W-23. Arch Biochem Biophys 116:358–367
    [Google Scholar]
  3. Cohan F. M., Roberts M. S., King E. C. 1991; The potential for genetic exchange by transformation within a natural population of Bacillus subtilis. Evolution 45:1393–1421
    [Google Scholar]
  4. Cummins C. S., Johnson J. L. 1971; Taxonomy of the clostridia: wall composition and DNA homologies in Clostridium butyricum and other butyric acid-producing clostridia. J Gen Microbiol 67:33–46
    [Google Scholar]
  5. De Ley J. H., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142
    [Google Scholar]
  6. Fox K. F., Wunschel D. S., Fox A., Stewart G. C. 1998; Complementarity of GC-MS and LC-MS analyses for determination of carbohydrate profiles of vegetative cells and spores of bacilli. J Microbiol Methods 33:1–11
    [Google Scholar]
  7. Haynes W.C., Wickerham L. J., Hesseltine C. W. 1955; Maintenance of cultures of industrially important microorganisms. Appl Microbiol 3:361–368
    [Google Scholar]
  8. Johnson J. L. 1973; Use of nucleic-acid homologies in the taxonomy of anaerobic bacteria. Int J Syst Bacteriol 23:308–315
    [Google Scholar]
  9. Lance D. G., Jones J. K. N. 1967; Gas chromatography of derivatives of the methyl esters of D-xylose. Can J Chem 45:1995–1998
    [Google Scholar]
  10. Lane D. L. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp. 115–175 Edited by Stackebrandt E., Goodfellow M. New York: Wiley;
    [Google Scholar]
  11. Majewski J., Cohan F. M. 1998; The effect of mismatch repair and heteroduplex formation on sexual isolation in Bacillus. Genetics 148:13–18
    [Google Scholar]
  12. Nakamura L. K. 1996; Paenibacillus apiarius sp. nov. Int J Syst Bacteriol 46:688–693
    [Google Scholar]
  13. Nakamura L. K., Swezey J. 1983; Taxonomy of Bacillus circulans Jordan 1890: base composition and reassociation of deoxyribonucleic acid. Int J Syst Bacteriol 33:46–52
    [Google Scholar]
  14. Roberts M. S., Cohan F. M. 1993; The effect of DNA sequence divergence on sexual isolation in Bacillus. Genetics 134:401–408
    [Google Scholar]
  15. Roberts M. S., Cohan F. M. 1995; Recombination and migration rates in natural populations of Bacillus subtilis and Bacillus mojavensis. Evolution 49:1081–1094
    [Google Scholar]
  16. Roberts M. S., Nakamura L. K., Cohan F. M. 1994; Bacillus mojavensis sp. nov., distinguishable from Bacillus subtilis by sexual isolation, divergence in DNA sequence, and differences in fatty acid composition. Int J Syst Bacteriol 44:256–264
    [Google Scholar]
  17. Roberts M. S., Nakamura L. K., Cohan F. M. 1996; Bacillus vallismortis sp. nov., a close relative of Bacillus subtilis, isolated from soil in Death Valley, California. Int J Syst Bacteriol 46:470–475
    [Google Scholar]
  18. Seki T., Chung C.-K., Mikami H., Oshima Y. 1978; Deoxyribonucleic acid homology and taxonomy of the genus Bacillus. Int J Syst Bacteriol 28:182–189
    [Google Scholar]
  19. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849
    [Google Scholar]
  20. Zawadzki P., Roberts M. S., Cohan F. M. 1995; The log-linear relationship between sexual isolation and sequence divergence in Bacillus transformation is robust. Genetics 140:917–932
    [Google Scholar]
/content/journal/ijsem/10.1099/00207713-49-3-1211
Loading
/content/journal/ijsem/10.1099/00207713-49-3-1211
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error