%0 Journal Article %A Scholten, Edzard %A Lukow, Thomas %A Auling, Georg %A Kroppenstedt, Reiner M. %A Rainey, Fred A. %A Diekmann, Hans %T Thauera mechernichensis sp. nov., an aerobic denitrifier from a leachate treatment plant %D 1999 %J International Journal of Systematic and Evolutionary Microbiology, %V 49 %N 3 %P 1045-1051 %@ 1466-5034 %R https://doi.org/10.1099/00207713-49-3-1045 %K aerobic denitrification %K Thauera mechernichensis sp. nov. %I Microbiology Society, %X A heterotrophic bacterial strain TL1T capable of aerobic denitrification was previously enriched in continuous culture from a landfill leachate treatment plant and isolated as a pure culture. The taxonomic position of this isolate within the β-subclass of the Proteobacteria was determined by 16S rDNA sequence analysis and by conventional taxonomy including substrate spectrum, quinone type (ubiquinone Q-8) and cellular fatty acid composition. Detection of the specific polyamine 2-hydroxyputrescine supports the membership of strain TL1T in the β-subclass of the Proteobacteria. The results of 16S rDNA sequencing showed that the strain clustered with, but was separate from, Thauera aromatica and Thauera selenatis. DNA-DNA hybridization experiments indicated that the new isolate represents a new species of the genus, for which the name Thauera mechernichensis is proposed; the type strain is DSM 12266T. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-49-3-1045