1887

Abstract

Three strains of moderately thermophilic, sulfur-reducing bacteria were isolated from shallow-water hot vents of the Bay of Plenty (New Zealand) and Matupi Harbour (Papua New Guinea). Cells of all isolates were short, Gram-negative, motile rods with one polar flagellum. All strains were obligate anaerobes and grew optimally at pH 5.8-6.2, 52-54 °C and 2.5-3% (w/v) NaCI. Growth substrates were molecular hydrogen, acetate and saturated fatty acids; one of the strains, isolated from Matupi Harbour, was able to utilize ethanol. Elemental sulfur was required for growth. HS and CO were the only growth products. No growth occurred in the absence of 100 mg yeast extract l. The G+C content of the DNA determined for the type strain MH was 40.4 mol%. Results of 16S rDNA sequencing indicated that these strains represent a distinct lineage most closely related to the genus . On the basis of the results of morphological, physiological and phylogenetic studies, a new genus, gen. nov., is proposed with the type species gen. nov., sp. nov., of which the type strain is MH (= DSM 10411).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-49-3-1033
1999-07-01
2024-07-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/49/3/ijs-49-3-1033.html?itemId=/content/journal/ijsem/10.1099/00207713-49-3-1033&mimeType=html&fmt=ahah

References

  1. Balch W. E., Fox G. E., Magrum L. J., Woese C. R., Wolfe R. S. 1979; Methanogens: reevaluation of a unique biological group. Microbiol Rev 43:260–296
    [Google Scholar]
  2. Bonch-Osmolovskaya E. A. 1994; Bacterial sulfur reduction in hot vents. FEMS Microbiol Rev 15:65–67
    [Google Scholar]
  3. Bonch-Osmolovskaya E. A., Sokolova T. G., Kostrikina N. A., Zavarzin G. A. 1990; Desulfurella acetivorans gen. nov., sp. nov. - a new thermophilic sulfur-reducing eubacterium. Arch Microbiol 153:151–155
    [Google Scholar]
  4. De Soete G. 1983; A least squares algorithm for fitting additive trees to proximity data. Psychometrika 48:621–626
    [Google Scholar]
  5. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism 321–132 Munro H. N. New York: Academic Press;
    [Google Scholar]
  6. Maidak B. L., Larsen N., McCaughey M. J., Overbeek R., Olsen G. J., Fogel K., Blandy J., Woese C. R. 1994; The Ribosomal Database Project. Nucleic Acids Res 22:3485–3487
    [Google Scholar]
  7. Miroshnichenko M. L., Gongadze G. A., Lysenko A. M., Bonch-Osmolovskaya E. A. 1994; Desulfurella multipotens sp. nov., a new sulfur-respiring thermophilic eubacterium from Raoul Island (Kermadec archipelago, New Zealand). Arch Microbiol 161:88–93
    [Google Scholar]
  8. Miroshnichenko M. L., Rainey F. A., Hippe H., Chernyh N. A., Kostrikina N. A., Bonch-Osmolovskaya E. A. 1998a; Desulfurella kamchatkensis sp. nov. and Desulfurella propionica sp. nov., new sulfur-respiring thermophilic bacteria from Kamchatka thermal environments. Int J Syst Bacterio! 48:475–479
    [Google Scholar]
  9. Miroshnichenko M. L., Kostrikina N. A., Hippe H., Slobodkin A. I., Bonch-Osmolovskaya E. A. 1998b; Biodiversity of thermophilic sulfur-reducing bacteria: new substrates and new habitats. Microbiology (English translation of Mikrobiologiyd) 67:563–568
    [Google Scholar]
  10. Rainey F. A., Toalster R., Stackebrandt E. 1993; Desulfurella acetivorans, a thermophilic, acetate-oxidizing and sulfur-reducing organism, represents a distinct lineage within the Proteobacteria. Syst Appl Microbiol 16:373–379
    [Google Scholar]
  11. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylo- genetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092
    [Google Scholar]
  12. Trüper H. G., Schlegel H. G. 1964; Sulfur metabolism in Thiorhodaceae. I. Quantitative measurements on growing cells of Chromatium okenii. J Microbiol Serol 30:225–232
    [Google Scholar]
  13. Widdel F., Pfennig N. 1992; The genus Desulfuromonas and other Gram-negative sulfur-reducing eubacteria. In The Pro-karyotes, 2. 13379–3389 Ballows A., Triiper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer:
    [Google Scholar]
  14. Wolin E. A., Wolin M. J., Wolfe R. S. 1963; Formation of methane by bacterial extracts. J Biol Chem 238:2882–2888
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-49-3-1033
Loading
/content/journal/ijsem/10.1099/00207713-49-3-1033
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error