sp. nov., a novel pathogen that affects Asian pear trees ( Nakai) Free

Abstract

A novel pathogen from Asian pears ( Nakai) was analysed by sequencing the 16S rDNA and the adjacent intergenic region, and the data were compared to related . The 16S rDNA of the Asian pear pathogen was almost identical with the sequence of , in contrast to the 16S–23S rRNA intergenic transcribed spacer region of both species. A dendrogram was deduced from determined sequences of the spacer regions including those of several related species such as subsp. and . Dendrograms derived from 121 biochemical characteristics including Biotype 100 data placed the Asian pear pathogen close to and more distantly to other members of the species and to the species and . Another DNA relatedness study was performed by DNA hybridizations and estimation of Δ values. The Asian pear strains constituted a tight DNA hybridization group (89–100%) and were barely related to strains of (40–50%) with a Δ in the range of 5·2–6·8. The G+C content of DNA from the novel pathogen is 52 mol %. Therefore, it is proposed that strains isolated from Asian pears constitute a new species and the name is suggested; the type strain is strain Ep16/96 (= CFBP 4172 = DSM 12163).

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1999-04-01
2024-03-29
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References

  1. Ayers S. H., Rupp P., Johnson T. 1919; A study of the alkaliforming bacteria. Milk USDA Bulletin;782
    [Google Scholar]
  2. Bereswill S., Pahl A., Bellemann P., Zeller W., Geider K. 1992; Sensitive and species-specific detection of Erwinia amylovora by polymerase chain reaction-analysis. Appl Environ Microbiol 58:3522–3526
    [Google Scholar]
  3. Bereswill S., Bugert P., Bruchmuller I., Geider K. 1995; Identification of Erwinia amylovora by PCR with chromosomal DNA. Appl Environ Microbiol 61:2636–2642
    [Google Scholar]
  4. Bereswill S., Jock S., Bellemann P., Geider K. 1997; Identification of Erwinia amylovora by growth in the presence of copper sulfate and by capsule staining with lectin. Plant Dis 82:158–164
    [Google Scholar]
  5. Brenner D. J., McWorter A. C., Leete Knutson J. K., Steigerwalt A. G. 1982; Escherichia vulneris: a new species of Enterobacteriaceae associated with human wounds. J Clin Microbiol 15:1133–1140
    [Google Scholar]
  6. Chung Y. R., Brenner D. J., Steigerwalt A. G., Kim B. S., Kim H. T., Cho K. Y. 1993; Enterobacter pyrinus sp.nov.anorganism associated with brown leaf spot disease of pear trees. Int J Syst Bacteriol 43:157–161
    [Google Scholar]
  7. Crosa J. M., Brenner D. J., Falkow S. 1973; Use of a single-strand-specific nuclease for analysis of bacterial and plasmid deoxyribonucleic acid homo- and heteroduplexes. J Bacteriol 115:904–911
    [Google Scholar]
  8. Falkenstein H., Bellemann P., Walter S., Zeller W., Geider K. 1988; Identification of Erwinia amylovora, the fireblight pathogen, by colony hybridization with DNA from plasmid pEA29. Appl Environ Microbiol 54:2798–2802
    [Google Scholar]
  9. Kwon S.-W., Go S.-J., Kang H.-W., Ryu J.-C., Jo J.-K. 1997; Phylogenetic analysis of Erwinia species based on 16S rRNA gene sequences. Int J Syst Bacteriol 47:1061–1067
    [Google Scholar]
  10. Leblond-Bourget N., Philippe H., Mangin I., Decaris B. 1996; 16S rRNA and 16S to 23S internal transcribed spacer sequence analysis reveal inter- and intraspecific Bifidobacterium phylogeny. Int J Syst Bacteriol 46:102–111
    [Google Scholar]
  11. Lelliott R. A., Stead D. E. 1987 Methods for the Diagnosis of Bacterial Diseases of Plants Oxford: Blackwell;
    [Google Scholar]
  12. Loughney K., Lund E., Dahlberg J. E. 1982; tRNA genes are found between 16S and 23S rRNA genes in the Bacillus subtilis . Nucleic Acids Res 10:1607–1624
    [Google Scholar]
  13. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118
    [Google Scholar]
  14. Owen R. J., Lapage S. P. 1976; The thermal denaturation of partly purified bacterial deoxyribonucleic acid and its taxo-nomic implications. J Appl Bacteriol 41:335–340
    [Google Scholar]
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