1887

Abstract

An aerobic bacterium, strain BN12, which degrades substituted naphthalenesulfonates and substituted salicylates was isolated from a 6-aminonaphthalene-2-sulfonate-degrading microbial consortium originating from the River Elbe, Germany. Chemotaxonomic investigations of quinones, polyamines and polar lipids allowed allocation of this strain to the -subclass of the and revealed similarity to species of the genera and This was confirmed by typing with 16S rRNA-targeted oligonucleotide probes and 16S rDNA sequencing and phylogenetic analysis, indicating that BN12 clusters most closely with a strain ‘’ THI 051 and with the above genera but comprising a separate branch. DNA–DNA hybridizations demonstrated that strain BN12 is different from all species of currently described and recognized. The fatty acid patterns, substrate utilization profile and biochemical characteristics displayed no obvious similarity to the characteristics of and species. ‘’ THI 051, however, revealed phenotypic similarities to BN12. Furthermore, 16S rRNA sequences of showed a high similarity to the 16S rRNA sequences of all currently recognized species. On the basis of these and previously published results, the new genus is proposed, harbouring the two new species sp. nov. and sp. nov. The type strains are BN12 ( = DSM 6986) and THI 051 (= IFO 1457), respectively.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-49-2-887
1999-04-01
2024-12-12
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/49/2/ijs-49-2-887.html?itemId=/content/journal/ijsem/10.1099/00207713-49-2-887&mimeType=html&fmt=ahah

References

  1. Amann R. I., Binder B. J., Olson R. J., Chrisholm S. W., Devereux R., Stahl D. A. 1990; Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl Environ Microbiol 56:1919–1925
    [Google Scholar]
  2. Auling G., Probst A., Kroppenstedt R. M. 1986; Chemo- and molecular taxonomy of d (–)-tartrate-utilizing pseudomonads. Syst Appl Microbiol 8:114–120
    [Google Scholar]
  3. Auling G., Busse H.-J., Egli T., El-Banna T., Stackebrandt E. 1993; Description of the Gram-negative, obligately aerobic, nitrilotriacetate (NTA)-utilizing bacteria as Chelatobacter heintzii gen. nov., sp. nov., and Chelatococcus asaccharovorans gen. nov., sp. nov. Syst Appl Microbiol 16:104–112
    [Google Scholar]
  4. Busse H.-J., El-Banna T., Auling G. 1989; Evaluation of different approaches for identification of xenobiotic-degrading pseudomonads. Appl Environ Microbiol 55:1578–1583
    [Google Scholar]
  5. Busse H.-J., Bunka S., Hensel A., Lubitz W. 1997; Discrimination of members of the family Pasteurellaceae based on polyamine patterns. Int J Syst Bacteriol 47:698–708
    [Google Scholar]
  6. Contzen M., Wittich R.-M., Knackmuss H.-J., Stolz A. 1996; Degradation of benzene 1,3-disulfonate by a mixed bacterial culture. FEMS Microbiol Lett 136:45–50
    [Google Scholar]
  7. Dangmann E., Stolz A., Kuhm A. E., Hammer A., Feigel B., Noisommit-Rizzi N., Rizzi M., Reuß M., Knackmuss H.-J. 1996; Degradation of 4-aminobenzenesulfonate by a two-species bacterial coculture. Biodegradation 7:223–229
    [Google Scholar]
  8. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142
    [Google Scholar]
  9. Feigel B. J., Knackmuss H.-J. 1988; Bacterial catabolism of sulfanilic acid via catechol-4-sulfonic acid. FEMS Microbiol Lett 55:113–117
    [Google Scholar]
  10. Feigel B. J., Knackmuss H.-J. 1993; Syntrophic interactions during degradation of 4-aminobenzenesulfonic acid by a two species bacterial culture. Arch Microbiol 159:124–130
    [Google Scholar]
  11. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791
    [Google Scholar]
  12. Felsenstein J. 1993; phylip (Phylogeny Inference Package) version 3.5C. Department of Genetics, University of Washington; Seattle, WA, USA:
    [Google Scholar]
  13. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. 1994 Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  14. Hamana K., Kishimoto N. 1996; Polyamine distribution patterns in C1 compound-utilizing eubacteria and acidophilic eubacteria. J Gen Appl Microbiol 42:431–437
    [Google Scholar]
  15. Hamana K., Minamisawa K., Matsuzaki S. 1990; Polyamines in Rhizobium Bradyrhizobium Azorhizobium and Agrobacterium. FEMS Microbiol Lett 71:71–76
    [Google Scholar]
  16. Jahnke M., Lehmann F., Schoebel A., Auling G. 1993; Transposition of TOL catabolic genes (Tn4651) into degradative plasmid pSAH of Alcaligenes sp. O-1 ensures simultaneous mineralization of sulpho- and methyl-substituted aromatics. J Gen Microbiol 139:1959–1966
    [Google Scholar]
  17. Jarvis B. D. W., Sivakumaran S., Tighe T. W., Gillis M. 1996; Identification of Agrobacterium and Rhizobium based on cellular fatty acid composition. Plant Soil 184:143–158
    [Google Scholar]
  18. Jarvis B. D. W., van Berkum P., Chen W. X., Nour S. M., Fernandez M. P., Cleyet-Marel J. C., Gillis M. 1997; Transfer of Rhizobium loti Rhizobium huakuii Rhizobium ciceri Rhizobium mediterraneum and Rhizobium tianshanense to Mesorhizobium gen. nov. Int J Syst Bacteriol 47:895–898
    [Google Scholar]
  19. Jimenez L., Breen A., Thomas N., Federle T. W., Sayler G. S. 1991; Mineralization of linear alkylbenzene sulfonate by a four-member aerobic bacterial consortium. Appl Environ Microbiol 57:1566–1569
    [Google Scholar]
  20. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism 321–132 Munro H. N. New York: Academic Press;
    [Google Scholar]
  21. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005
    [Google Scholar]
  22. Kämpfer P., Denner E. B. M., Meyer S., Moore E. R. B., Busse H.-J. 1997; Classification of “Pseudomonas azotocolligans” Anderson 1955, 132 in the genus Sphingomonas as Sphingomonas trueperi sp. nov. Int J Syst Bacteriol 47:577–583
    [Google Scholar]
  23. Kaneko T., Katoh K., Fujimoto M., Kumagi M., Tamaoka J., Katayama-Fujimura Y. 1986; Determination of the nucleotide composition of a deoxyribonucleic acid by high-performance liquid chromatography of its enzymatic hydrolysates : a review. J Microbiol Methods 4:229–240
    [Google Scholar]
  24. Katayama-Fujimura Y., Enokizono Y., Kaneko T., Kuraishi H. 1983; Deoxyribonucleic acid homologies among species of the genus Thiobacillus. J Gen Appl Microbiol 29:287–295
    [Google Scholar]
  25. Katayama-Fujimura Y., Tsuzaki N., Hirata A., Kuraishi H. 1984; Polyhedral inclusion bodies (carboxysomes) in Thiobacillus species with reference to the taxonomy of the genus Thiobacillus. J Gen Appl Microbiol 30:211–222
    [Google Scholar]
  26. Kroppenstedt R. M. 1982; Anwendung chromatographischer HP-Verfahren (HPTLC und HPLC) in der Bakterien-taxonomie. GIT Lab Med 5:266–275
    [Google Scholar]
  27. Kuhm A. E., Stolz A., Ngai K.-L., Knackmuss H.-J. 1991; Purification and characterization of a 1,2-dihydroxy-naphthalene dioxygenase from a bacterium that degrades naphthalenesulfonic acids. J Bacteriol 173:3795–3802
    [Google Scholar]
  28. Kuhm A. E., Knackmuss H.-J., Stolz A. 1993; Purification and properties of 2′-hydroxybenzalpyruvate aldolase from a bacterium that degrades naphthalenesulfonates. J Biol Chem 268:9484–9489
    [Google Scholar]
  29. de Lajudie P., Willems A., Nick G. 9 other authors 1998; Characterization of tropical tree rhizobia and description of Mesorhizobium plurifarium sp. nov. Int J Syst Bacteriol 48:369–382
    [Google Scholar]
  30. Ludwig W., Amann R., Martinez-Romero E., Schonhuber W., Bauer S., Neef A., Schleifer K.-H. 1998; rRNA-based identification and detection systems for Rhizobia and other bacteria. Plant Soil 204:1–19
    [Google Scholar]
  31. Manz W., Amann R., Ludwig W., Wagner M., Schleifer K.-H. 1992; Phylogenetic oligodeoxynucleotide probes for the major subclasses of Proteobacteria problems and solutions. Syst Appl Microbiol 15:593–600
    [Google Scholar]
  32. Nörtemann B. 1987 Bakterieller Abbau von Amino- und Hydroxynaphthalinsulfonsàuren. PhD thesis Universitàt Stuttgart;
    [Google Scholar]
  33. Nörtemann B., Baumgarten J., Rast H. G., Knackmuss H.-J. 1986; Bacterial communities degrading amino- and hydroxy-naphthalenesulfonates. Appl Environ Microbiol 52:1195–1202
    [Google Scholar]
  34. Nörtemann B., Kuhm A. E., Knackmuss H.-J., Stolz A. 1994; Conversion of substituted naphthalenesulfonates by Pseudomonas sp. BN6. Arch Microbiol 161:320–327
    [Google Scholar]
  35. Pearson W. R., Lipman D. J. 1988; Improved tools for biological sequence comparison. Proc Natl Acad SciUSA 85:2444–2448
    [Google Scholar]
  36. Saitou N., Nei M. 1987; The neighbor-joining method : a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  37. Sneath P. H. A., Sokal R. R. 1973 Numerical Taxonomy San Francisco: W. H. Freeman;
    [Google Scholar]
  38. Stolz A., Knackmuss H.-J. 1993a; Bacterial metabolism of 5-aminosalicylic acid: enzymatic conversion to l-malate, pyruvate and ammonia. J Gen Microbiol 139:1019–1025
    [Google Scholar]
  39. Stolz A., Knackmuss H.-J. 1993b; Degradation of 2,4-dihydroxybenzoate by Pseudomonas sp. BN9. FEMS Microbiol Lett 108:219–224
    [Google Scholar]
  40. Stolz A., Nörtemann B., Knackmuss H.-J. 1992; Bacterial metabolism of 5-aminosalicylic acid: Initial ring-cleavage. Biochem J 282:675–680
    [Google Scholar]
  41. Thompson J. D., Higgins D. G., & Gibson T. J. 1994; clustalw- Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
    [Google Scholar]
  42. Thurnheer T., Cook A. M., Leisinger T. 1988; Coculture of defined bacteria to degrade seven sulfonated aromatic compounds: efficiency, rates and phenotypic variations. Appl Microbiol Biotechnol 29:605–609
    [Google Scholar]
  43. Tindall B. J. 1990; Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66:199–202
    [Google Scholar]
  44. Urakami T., Araki H., Oyanagi H., Suzuki K.-I., Komagata K. 1992; Transfer of Pseudomonas aminovorans (den Dooren de Jong 1926) to Aminobacter gen. nov. as Aminobacter aminovorans comb. nov. and description of Aminobacter aganoensis sp. nov. and Aminobacter niigataensis sp. nov. Int J Syst Bacteriol 42:84–92
    [Google Scholar]
  45. Zürrer D., Cook A., Leisinger T. 1987; Microbial desulfonation of substituted naphthalenesulfonic acids and benzenesulfonic acids. Appl Environ Microbiol 53:1459–1463
    [Google Scholar]
/content/journal/ijsem/10.1099/00207713-49-2-887
Loading
/content/journal/ijsem/10.1099/00207713-49-2-887
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error