DNA relatedness was determined among 303 strains of and . Included in the analysis were reference strains from 228 well-characterized and recognized serovars. The study included 268 serovars from 29 named and one or more unnamed serogroups. The strains clustered into 17 DNA hybridization groups, representing 12 previously described species (292 strains) and five new genomospecies (11 strains). The largest groups included (91 strains from 82 serovars), (61 strains from 59 serovars), (49 strains from 43 serovars), (29 strains from 26 serovars) and (20 strains from 20 serovars). The new genomospecies include genomospecies 1 (two strains, serovars pinagchang and sichuan), genomospecies 2 (six strains, serovars lushui, manhao 3, manzhuang, nanding, mengla and yunnan), genomospecies 3 (one strain, serovar holland), genomospecies 4 (one strain, serovar hualin) and genomospecies 5 (one strain, serovar saopaulo). With the exception of Ballum, all serogroups with greater than one serovar studied were genetically heterogeneous. Phenotypic tests, including optimal growth temperature, lipase activity and growth inhibition by copper sulfate or 2,6-diaminopurine, were of little use in differentiating DNA relatedness groups. The name sp. nov. is proposed for genomospecies 2 (type strain L 60= ATCC 700520, serovar manhao 3).


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