strain Z: growth, antigenic and genome characteristics Free

Abstract

is the type species of , a recently proposed family in the order . In the current study, growth, antigenic and genomic characteristics of this intracellular bacterium were investigated and compared to those of members of the family . Growth of the organism, as assessed by infectivity assays, reached a plateau in 2–3 d although by light microscopy the cytopathic effect on the host cells increased for 12 or more days after infection. . growth was unaffected by sulfadiazine. Cells infected by . strain Z were not recognized by either of two monoclonal antibodies specific for LPS and several specific primers were unable to PCR amplify a . gene. The . genome contained one copy of the ribosomal operon. The genome size of . strain Z was determined by PFGE to be 1·7 Mbp, and the G+C content was 42·5 mol%. These data, taken together with other published data, are consistent with the proposal that . belongs to a distinct family in the order .

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1999-04-01
2024-03-28
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