Simkania negevensis is the type species of Simkaniaceae, a recently proposed family in the order Chlamydiales. In the current study, growth, antigenic and genomic characteristics of this intracellular bacterium were investigated and compared to those of members of the family Chlamydiaceae. Growth of the organism, as assessed by infectivity assays, reached a plateau in 2–3 d although by light microscopy the cytopathic effect on the host cells increased for 12 or more days after infection. S. negevensis growth was unaffected by sulfadiazine. Cells infected by S. negevensis strain ZT were not recognized by either of two monoclonal antibodies specific for Chlamydiaceae LPS and several specific Chlamydiaceae ompA primers were unable to PCR amplify a S. negevensis gene. The S. negevensis genome contained one copy of the ribosomal operon. The genome size of S. negevensis strain ZT was determined by PFGE to be 1·7 Mbp, and the G+C content was 42·5 mol%. These data, taken together with other published data, are consistent with the proposal that S. negevensis belongs to a distinct family in the order Chlamydiales.
AndersenA. A.,
Van DeusenR. A.1988; Production and partial characterization of monoclonal antibodies to four Chlamydia psittaci isolates. Infect Immun 56:2075–2079
AmannR.,
SpringerN.,
SchonhuberW.,
LudwigW.,
SchmidE. N.,
MullerK. D.,
MichelR.1997; Obligate intracellular bacterial parasites of acanthamoebae related to Chlamydia spp. Appl Environ Microbiol 63:115–121
CaldwellH. D.,
KromhoutJ.,
SchachterJ.1981; Purification and partial characterization of the major outer membrane protein of Chlamydia trachomatis. Infect Immun 31:1161–1176
DenamurE.,
SayadaC.,
SouriauA.,
OrfilaJ.,
RodolakisA.,
ElionJ.1991; Restriction pattern of the major outer-membrane protein gene provides evidence for a homogeneous invasive group among ruminant isolates of Chlamydia psittaci. J Gen Microbiol 137:2525–2530
EverettK. D. E.,
AndersenA. A.1997; The ribosomal intergenic spacer and domain I of the 23S rRNA gene are phylogenetic markers for Chlamydia spp. Int J Syst Bacteriol 47:461–473
EverettK. D. E.,
BushR. M.,
AndersenA. A.1999; Emended description of the order Chlamydiales proposal of Parachlamydiaceae fam. nov. and Simkaniaceae fam. nov., each containing one monotypic genus, revised taxonomy of the family Chlamydiaceae including a new genus and five new species, and standards for the identification of organisms. Int J Syst Bacteriol 49:415–440
FriedmanM. G.,
GalilA.,
GreenbergS.,
KahaneS.1999; Seroprevalence of IgG antibodies to the chlamydia-like microorganism ‘Simkania Z’ by ELISA. Epidemiol Infect 122:117–123
FuY.,
BaumanM.,
KosmaP.,
BradeL.,
BradeH.1992; A synthetic glycoconjugate representing the genus-specific epitope of chlamydial lipopolysaccharide exhibits the same specificity as its natural counterpart. Infect Immun 60:1314–1321
KahaneS.,
GonenR.,
Sayada C.,
ElionJ.,
FriedmanM. G.1993; Description and partial characterization of a new chlamydia-like microorganism. FEMS Microbiol Lett 109:329–334
KahaneS.,
MetzerE.,
FriedmanM. G.1995; Evidence that the novel microorganism “Z” may belong to a new genus in the family Chlamydiaceae. FEMS Microbiol Lett 126:203–208
KahaneS.,
GreenbergD.,
LiebermanD.,
DaganR.,
HaikinH.,
BirY.,
FriedmanM. G.1996; The novel microorganism “Z” is capable of infecting humans. In Proceedings of the Third Meeting of the European Society for Chlamydia Research18StaryA.Vienna: Study Group for STD and Dermatological Microbiology of the Austrian Society for Dermatology and Venerology
KahaneS.,
GreenbergD.,
FriedmanM. G.,
HaikinH.,
DaganR.1998; High prevalence of Snegevensis a novel chlamydia-like bacterium, in infants with acute bronchiolitis. J Infect Dis 177:1425–1429
LiebermanD.,
KahaneS.,
LiebermanD.,
FriedmanM. G.1997; Pneumonia with serological evidence of acute infection with the chlamydia-like microorganism “Z”. Am J Respir Crit Care Med 156:578–582
MoulderJ. W.,
HatchT. P.,
KuoC.-C.,
SchachterJ.,
StorzJ.1984; Genus Chlamydia. In Bergey’s Manual of Systematic Bacteriology1729–739KriegN. R.,
HoltJ. G.
Baltimore: Williams & Wilkins;
PetterssonB.,
AnderssonA.,
LeitnerT.,
OlsvikO.,
UhlénM.,
StoreyC.,
BlackC. M.1997; Evolutionary relationships among members of the genus Chlamydia based on 16S ribosomal DNA analysis. J Bacteriol 179:4195–4205
StantonT. B.,
Fournie-AmazouzE.,
PosticD.,
TrottD. J.,
GrimontP. A.,
BarantonG.,
HampsonD. J.,
Saint GironsI.1997; Recognition of two new species of intestinal spirochetes : Serpulina intermedia sp. nov. and Serpulina murdochii sp. nov. Int J Syst Bacteriol 47:1007–1012