%0 Journal Article %A Rheims, Holger %A Frühling, Anja %A Schumann, Peter %A Rohde, Manfred %A Stackebrandt, Erko %T Bacillus silvestris sp. nov., a new member of the genus Bacillus that contains lysine in its cell wall %D 1999 %J International Journal of Systematic and Evolutionary Microbiology, %V 49 %N 2 %P 795-802 %@ 1466-5034 %R https://doi.org/10.1099/00207713-49-2-795 %K Bacillus RNA group 2 %K Bacillus silvestris %K 16S rDNA analysis %K phylogeny %K chemotaxonomy %I Microbiology Society, %X A Gram-positive, aerobic, rod-shaped, peritrichously flagellated, roundendospore-forming bacterium was isolated from a forest soil near Braunschweig, Lower Saxony, Germany, and designated strain HR3-23T(T = type strain). Morphologically, strain HR3-23T shows the characteristics of a member of the genus Bacillus. The spore position is terminal in a swollen sporangium. Comparative analysis of the 16S rDNA sequence shows strain HR3-23T to be most closely related to Caryophanon tenue (95·8% 16S rRNA similarity) and to Bacillus sphaericus (95·4% 16S rRNA similarity). Phylogenetically, the isolate clusters among species of Bacillus RNA group 2. The DNA G+C content of isolate HR3-23T is 39·3 mol%, the peptidoglycan type is A4α (l-Lys-d-Glu), the major respiratory lipoquinone is menaquinone MK-7 and the predominant fatty acid is of the iso-C15:0 type. Based on the morphological, chemotaxonomic, physiological and phylogenetic properties, a new species, Bacillus silvestris, is proposed; strain HR3-23Tis the type strain (= DSM 12223T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-49-2-795