1887

Abstract

A Gram-positive, aerobic, rod-shaped, peritrichously flagellated, roundendospore-forming bacterium was isolated from a forest soil near Braunschweig, Lower Saxony, Germany, and designated strain HR3-23(T = type strain). Morphologically, strain HR3-23 shows the characteristics of a member of the genus The spore position is terminal in a swollen sporangium. Comparative analysis of the 16S rDNA sequence shows strain HR3-23 to be most closely related to (95·8% 16S rRNA similarity) and to (95·4% 16S rRNA similarity).

Phylogenetically, the isolate clusters among species of RNA group 2. The DNA G+C content of isolate HR3-23 is 39·3 mol%, the peptidoglycan type is A4α (-Lys--Glu), the major respiratory lipoquinone is menaquinone MK-7 and the predominant fatty acid is of the iso-C type. Based on the morphological, chemotaxonomic, physiological and phylogenetic properties, a new species, , is proposed; strain HR3-23is the type strain (= DSM 12223).

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1999-04-01
2024-12-14
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References

  1. Ash C., Farrow J. A. E., Wallbanks S., Collins M. D. 1991; Phylogenetic heterogeneity of the genus Bacillus as revealed by comparative analysis of small-subunit-ribosomal RNA sequences. Lett Appl Microbiol 13:202–206
    [Google Scholar]
  2. Ash C., Priest F. G., Collins M. D. 1993; Molecular identification of rRNA group 3 bacilli (Ash, Farrow and Collins) using a PCR probe test. Antonie Leeuwenhoek 64:253–260
    [Google Scholar]
  3. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of the 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci USA 75:4801–4805
    [Google Scholar]
  4. Claus D., Berkeley R. C. W. 1986; Genus Bacillus Cohn 1872. In Bergey’s Manual of Systematic Bacteriology 21105–1140 Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  5. Claus D., Fahmy F. 1986; Genus Sporosarcina Kluyver and van Niel 1936, 401al. In Bergey’s Manual of Systematic Bacteriology 21202–1206 Sneath P. H. A., Mair N. S., Sharpe M. E., Holt. J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  6. Claus D., Fritze D. 1989; Taxonomy of Bacillus. In Bacillus, Biotechnology Handbooks 25–26 Harwood. C. R. New York: Plenum;
    [Google Scholar]
  7. Clausen V., Jones J. G., Stackebrandt E. 1985; 16S ribosomal RNA analysis of Filibacter limicola indicates a close relationship to the genus Bacillus. J Gen Microbiol 131:2659–2663
    [Google Scholar]
  8. Collins M. D., Jones D. 1979; Isoprenoid quinone composition as a guide to the classification of Sporolactobacillus and possibly related bacteria. J Appl Bacterial 47:293–297
    [Google Scholar]
  9. Collins M. D., Jones D. 1980; Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48:459–470
    [Google Scholar]
  10. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230
    [Google Scholar]
  11. De Soete G. 1983; A least squares algorithm for fitting additive trees to proximity data. Psychometrika 48:621–626
    [Google Scholar]
  12. Fahmy F., Flossdorf J., Claus D. 1985; The DNA base composition of the type strains of the genus Bacillus. Syst Appl Microbiol 6:60–65
    [Google Scholar]
  13. Farrow J. A. E., Ash C., Wallbanks S., Collins M. D. 1992; Phylogenetic analysis of the genera Planococcus, Marinococcus and Sporosarcina and their relationships to members of the genus Bacillus. FEMS Microbiol Lett 93:167–172
    [Google Scholar]
  14. Farrow J. A. E., Wallbanks S., Collins M. D. 1994; Phylogenetic interrelationships of round-spore-forming bacilli containing cell walls based on lysine and the non-spore-forming genera Caryophanon Exiguobacterium Kurthia and Planococcus. Int J Syst Bacteriol 44:74–82
    [Google Scholar]
  15. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–789
    [Google Scholar]
  16. Felsenstein J. 1993; phylip: Phylogeny inference package, version 3.5.1. Department of Genetics University of Washington; Seattle, WA, USA.:
    [Google Scholar]
  17. Gordon R. E., Haynes W. C., Pang C. H. 1973; The genus Bacillus. Agricultural Handbook427 Washington, DC: USDA.;
    [Google Scholar]
  18. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. 1996; Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239
    [Google Scholar]
  19. Hao M. V., Kocur M., Komagata K. 1985; A new species of Planococcus P kocurii, isolated from fish, frozen foods and fish curing brine. J Gen Microbiol 31:441–455
    [Google Scholar]
  20. Heimbrook M. E., Wang W. L L., Campbell G. 1989; Staining bacterial flagella easily. Clin Microbiol 27:2612–2615
    [Google Scholar]
  21. Hess A., Hollander R., Mannheim W. 1979; Lipoquinones of some spore-forming rods, lactic acid bacteria and actinomycetes. J Gen Microbiol 115:247–252
    [Google Scholar]
  22. Heyndrickx M., Lebbe L., Kersters K., DeVos P., Forsyth G., Logan N. A. 1998; Virgibacillus: a new genus to accommodate Bacillus pantothenticus (Proom and Knight 1950). Emended description of Virgibacillus pantothenticus. Int J Syst Bacteriol 48:99–106
    [Google Scholar]
  23. Holt J. G., Krieg N. R., Sneath P. H. A., Staley J. T., Williams S. T. 1994; Group 19, regular, nonsporing Gram-positive rods. In Bergey’s Manual of Determinative Bacteriology, 9.565–570 Baltimore: Williams & Wilkins;
    [Google Scholar]
  24. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism21–132 Munro H. N. New York: Academic Press;
    [Google Scholar]
  25. Kocur M. 1986; Genus Planococcus. In Bergefs Manual of Systematic Bacteriology 21011–1013 Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  26. Lanyi B. 1989; Characterization tests appropriate for numerical taxonomic studies. Methods Microbiol 19:1–67
    [Google Scholar]
  27. MacKenzie S. L. 1987; Gas chromatographic analysis of amino acids as the N-heptafluorobutyryl isobutyl esters. J Assoc Off Anal Chem 70:151–160
    [Google Scholar]
  28. Maidak B. L., Olsen G. J., Larsen N., Overbeek R., McCaughey M. J., Woese C. R. 1997; The RDP (Ribosomal Database Project). Nucleic Acids Res 25:109–111
    [Google Scholar]
  29. Maiden M. F. J., Jones J. G. 1984; A new filamentous, gliding bacterium, Filibacter limicola gen. nov., sp. nov., from lake sediment. J Gen Microbiol 130:2943–2959
    [Google Scholar]
  30. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G + C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167
    [Google Scholar]
  31. Minnikin D. E., Alshamaony L., G oodfellow M. 1975; Differentiation of Mycobacterium, Nocardia, and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. J Gen Microbiol 88:200–204
    [Google Scholar]
  32. Minnikin D. E., Collins M. D., Goodfellow M. 1979; Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47:87–95
    [Google Scholar]
  33. Pechman K. J., Lewis B. J., Woese C. R. 1976; Phylogenetic status of Sporosarcina ureae. Int J Syst Bacteriol 26:305–310
    [Google Scholar]
  34. Priest F. G., Goodfellow M., Todd C. 1988; A numerical classification of the genus Bacillus. J Gen Microbiol 134:1847–1882
    [Google Scholar]
  35. Rainey F. A., Stackebrandt E. 1993; Phylogenetic evidence for the relationship of Saccharococcus thermophilus to Bacillus thermoglucosidasius, Bacillus kaustophilus and Bacillus stearothermophilus. Syst Appl Microbiol 16:224–226
    [Google Scholar]
  36. Rainey F. A., Fritze D., Stackebrandt E. 1994; The phylogenetic diversity of thermophilic members of the genus Bacillus, as revealed by 16S rDNA analysis. FEMS Microbiol Lett 115:205–212
    [Google Scholar]
  37. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092
    [Google Scholar]
  38. Ranftl H., Kandler O. 1970; d-aspartyl-l-alanin als Interpeptidbrucke im Murein von Bacillus pasteurii Migula. Z Naturforsch 28C:4–8
    [Google Scholar]
  39. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  40. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–77
    [Google Scholar]
  41. Shaw S., Keddie R. M. 1983; A numerical taxonomic study of the genus Kurthia with a revised description of Kurthia zopfii and a description of Kurthia gibsonii sp. nov. Syst Appl Microbiol 4:253–276
    [Google Scholar]
  42. Shida O., Takagi H., Kadowaki K., Komagata K. 1996; Proposal for two new genera, Brevibacillus gen. nov. and Aneurinibacillus gen. nov. Int J Syst Bacteriol 46:939–946
    [Google Scholar]
  43. Sneath P. H. A. 1986; Section 13. Endospore-forming Gram-positive rods and cocci. In Bergey’s Manual of Systematic Bacteriology 21104–1105 Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  44. Spurr A. R. 1969; A low viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26:31–43
    [Google Scholar]
  45. Stackebrandt E., Goebel B. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849
    [Google Scholar]
  46. Stackebrandt E., Ludwig W., Weizenegger M., Dorn S., McGill T. J., Fox G. E., Woese C. E., Schubert W., Schleifer K.-H. 1987; Comparative 16S rRNA oligonucleotide analyses and murein types of round-spore-forming bacilli and non-spore-forming relatives. J Gen Microbiol 133:2523–2529
    [Google Scholar]
  47. Stead D. E., Sellwood J. E., Wilson J., Viney I. 1992; Evaluation of a commercial identification system based on fatty acid profiles for rapid, accurate identification of plant patho-genic bacteria. J Appl Bacteriol 72:315–321
    [Google Scholar]
  48. Trentini W. C. 1986; Genus Caryophanon Peshkoff 1939,244AL. In Berge/s Manual of Systematic Bacteriology 21259–1260 Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  49. Wisotzkey J. D., Jurtshuk P., Jr. Fox G. E., Deinhard G., Poralla K. 1992; Comparative sequence analysis on the 16S rRNA of Bacillus acidocaldarius, Bacillus acidoterrestris and Bacillus cycloheptanicus: proposal for a new genus Alicyclobacillus gen. nov. Int J Syst Bacteriol 42:263–269
    [Google Scholar]
  50. Yamada K., Komagata K. 1972; Taxonomic studies on coryneform bacteria. IV. Morphological, cultural, biochemical, and physiological characteristics. J Gen Appl Microbiol 18:399–416
    [Google Scholar]
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