gen. nov., sp. nov., a thermophilic, facultatively chemolithoautotrophic, hydrogen-oxidizing bacterium Free

Abstract

The taxonomic positions of ‘’ strain TH-1 and ‘’ strain TH-4 were studied by 16S rDNA sequencing. These organisms are Gram-negative, strictly aerobic, thermophilic facultatively chemolithoautotrophic hydrogen-oxidizing rods and have a DNA G+C content of 63–65 mol%. The major isoprenoid quinone is ubiquinone-8 an 3-hydroxy decanoic acid (3-OH C) is the major 3-hydroxy cellular fatty acid, phylogenetic analysis based on 16S rDNA sequences placed strains TH-1and TH-4 in the -subclass of the The taxonomic characteristics of these organisms are different from those of previously described aerobic, facultatively chemolithoautotrophic, hydrogen-oxidizing bacteria that belong to the -subclass of On the basis of the information described above, a new genus and species, gen. nov., sp. nov., is described to include both strains. The type strain is strain TH-1(= IFO 14978).

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1999-04-01
2024-03-29
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References

  1. Aragno M. 1992; Thermophilic, aerobic, hydrogen-oxidizing (Knallgas) bacteria. The Prokaryotes, 2.3917–3933 Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. New York: Springer Verlag;
    [Google Scholar]
  2. Aragno M., Schlegel H. G. 1992; The mesophilic hydrogen-oxidizing (Knallgas) bacteria. The Prokaryotes, 2.344–384 Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. New York: Springer Verlag;
    [Google Scholar]
  3. Busse H.-J., Auling G. 1992; The genera Alcaligenes and ‘Achromobacter’. The Prokaryotes, 2.2544–2555 Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. New York: Springer Verlag;
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791
    [Google Scholar]
  6. Goto E., Kodama T., Minoda Y. 1977; Isolation and culture conditions of thermophilic hydrogen bacteria. Agric Biol Chem 41:685–690
    [Google Scholar]
  7. Goto E., Kodama T., Minoda Y. 1978; Growth and taxonomy of thermophilic hydrogen bacteria. Agric Biol Chem 42:1305–1308
    [Google Scholar]
  8. Hamana K., Matsuzaki S., Niitsu M., Samejima K., Igarashi Y., Kodama T. 1991; Distribution of hydroxypolyamines, aminopropylcadaverine, and spermine in thermophilic, hydrogen-oxidizing pseudomonads. J Gen Appl Microbiol 37:431–437
    [Google Scholar]
  9. Huber R., Wilharm T., Huber D., Trincone A., Burggaf S., König H., Rachel R., Rockinger I., Fricke H., Stetter K. O. 1992; Aquifex pyrophilus gen. nov., sp. nov., represents a novel group of marine hyperthermophilic hydrogen-oxidizing bacteria. Syst Appl Microbiol 15:340–351
    [Google Scholar]
  10. Ishida T., Yokota A., Sugiyama J. 1997; Phylogenetic relationships of filamentous cyanobacterial taxa inferred from 16S rRNA sequence divergence. J Gen Appl Microbiol 43:237–241
    [Google Scholar]
  11. Kawasumi T., Igarashi Y., Kodama T., Minoda Y. 1984; Hydrogenobacter thermophilus gen. nov., sp. nov., an extremely thermophilic, aerobic, hydrogen-oxidizing bacterium. Int J Syst Bacteriol 34:5–10
    [Google Scholar]
  12. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120
    [Google Scholar]
  13. Kryukov V. R., Savel’eva N. D., Pusheva M. A. 1983; Calder o-bacterium hydrogenophilum gen. et sp. nov., an extremely thermophilic hydrogen bacterium and its hydrogenase activity. Microbiology 52:611–618 English translation of Mikrob-iologiya
    [Google Scholar]
  14. Nishihara H., Igarashi Y., Kodama T. 1991; Hydrogenovibrio marinus gen. nov., sp. nov., a marine obligately chemolitho-autotrophic hydrogen-oxidizing bacterium. Int J Syst Bacteriol 41:130–133
    [Google Scholar]
  15. Oyaizu-Masuchi Y., Komagata K. 1988; Isolation of free-living nitrogen-fixing bacteria from the rhizosphere of rice. J Gen Appl Microbiol 34:127–164
    [Google Scholar]
  16. Saitou N., Nei M. 1987; The neighbor-joining method : a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  17. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  18. Schlegel H. G. 1989; Aerobic hydrogen-oxidizing (Knallgas) bacteria. Autotrophic Bacteria305–329 Schlegel H. G., Bowien B. Madison, WI: Science Tech;
    [Google Scholar]
  19. Shima S., Suzuki K.-I. 1993; Hydrogenobacter acidophilus sp. nov., a thermoacidophilic, aerobic, hydrogen-oxidizing bacterium requiring elemental sulfur for growth. Int J Syst Bacteriol 43:703–708
    [Google Scholar]
  20. Takeuchi M., Yokota A. 1991; Reclassification of strains of Flavobacterium-Cytophaga group in IFO culture collection. Inst Ferment Res Commun (Osaka) 15:83–96
    [Google Scholar]
  21. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w : improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
    [Google Scholar]
  22. Urakami T., Araki H., Komagata K. 1995; Characteristics of newly isolated Xanthobacter strains and fatty acid compositions and quinone system in yellow-pigmented hydrogen-oxidizing bacteria. Int J Syst Bacteriol 45:863–867
    [Google Scholar]
  23. Willems A., Busse J., Goor M., Pot B., Falsen E., Jantzen E., Hoste B., Gillis M., Kersters K., Auling G., De Ley J. 1989; Hydrogenophaga a new genus of hydrogen-oxidizing bacteria that includes Hydrogenophaga flava comb. nov. (formerly Pseudomonas flava, Hydrogenophaga palleroniiformerly Pseudomonas palleronii, Hydrogenophaga pseudoflavaformerly Pseudomonas pseudoflava and ‘Pseudomonas carboxydo-flava’), and Hydrogenophaga taeniospiralisformerly Pseudomonas taeniospiralis. Int J Syst Bacteriol 39:319–333
    [Google Scholar]
  24. Willems A., Falsen E., Pot B., Jantzen E., Hoste B., Vandamme P., Gillis M., Kersters K., De Ley J. 1990; Acidovorax a new genus for Pseudomonas facilis Pseudomonas delafieldii E. Falsen (EF) group 13, EF group 16, and several clinical isolates, with the species Acidovorax facilis comb, nov., Acidovorax delafieldii comb, nov., and Acidovorax temperans sp. nov. Int J Syst Bacteriol 40:384–398
    [Google Scholar]
  25. Yabuuchi E., Kosako Y., Yano I., Hotta H., Nishiuchi Y. 1995; Transfer of two Burkholderia and an Alcaligenes species to Ralstonia gen. nov. : proposal of Ralstonia pickettiiRalston, Palleroni and Doudoroff 1973) comb, nov., Ralstonia solan-acearumSmith 1896) comb. nov. and Ralstonia eutrophaDavis 1969) comb. nov. Microbiol Immunol 39:897–904
    [Google Scholar]
  26. Yokoyama K., Hayashi N. R., Arai H., Chung S.-Y., Igarashi Y., Kodama T. 1995; Genes encoding RubisCO in Pseudomonas hydrogenothermophila are followed by a novel cbbQ gene similar to nirQ of the denitrification gene cluster from Pseudomonas species. Gene 153:75–79
    [Google Scholar]
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