1887

Abstract

The nucleotide sequences of the 16S rRNA gene (rDNA) in 38 taxa of the genus were compared phylogenetically. Based on phylogenetic tree analysis, staphylococcal species were divided into 12 cluster groups. These cluster groups were in very good agreement with species groups determined by DNA–DNA reassociation studies. These genealogical classifications were consistent with the results of the production of coagulase or oxidase and with resistance to novobiocin. These suggest that the phylogenetic relationship of the genus is accurately represented by the results obtained from the sequence analysis of 16S rDNA.

Keyword(s): 16S rDNA , phylogeny and Staphylococcus
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1999-04-01
2022-05-28
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References

  1. Bannerman T. L., Kloos W. E. 1991; Staphylococcus capitis subsp. ureolyticus subsp. nov. from human skin. Int J Syst Bacteriol 41:144–147
    [Google Scholar]
  2. Brosius J., Palmer J. L., Kennedy J. P., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci USA 75:4801–4805
    [Google Scholar]
  3. Chesneau O., Morvan A., Grimont F., Labischinski H., El Solh N. 1993; Staphylococcus pasteuri sp. nov., isolation from human, animal, and food specimens. Int J Syst Bacteriol 43:237–244
    [Google Scholar]
  4. De Buyser M.-L., Morvan A., Aubert S., Dilasser F., El Solh N. 1992; Evaluation of a ribosomal RNA gene probe for the identification of species and subspecies within the genus Staphylococcus. J Gen Microbiol 138:889–899
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using bootstrap. Evolution 39:783–791
    [Google Scholar]
  6. Foster G., Ross H. M., Hutson R. A., Collins M. D. 1997; Staphylococcus lutrae sp. nov., a new coagulase-positive species isolated from otters. Int J Syst Bacteriol 47:724–726
    [Google Scholar]
  7. Hájek V., Ludwig W., Schleifer K. H., Springer N., Zitzelsberger W., Kroppenstedt R. M., Kocur M. 1992; Staphylococcus muscae a new species isolated from flies. Int J Syst Bacteriol 42:97–101
    [Google Scholar]
  8. Igimi S., Takahashi E., Mitsuoka T. 1990; Staphylococcus schleiferi subsp. coagulans subsp. nov., isolated from the external auditory meatus of dogs with external ear otitis. Int J Syst Bacteriol 40:409–411
    [Google Scholar]
  9. Jordens J. Z. 1998; Genomic DNA digestion and ribotyping. Methods in Molecular Medicine 15 Molecular Bacteriology: Protocols and Clinical Applications 17–31 Woodford N., Johnson P. Totowa, NJ: Humana Press;
    [Google Scholar]
  10. Kimura M. 1980; A simplified method for estimating evolutionary rates of base substitution through comparative studies of nucleotide sequences. J Mol Evol 16:111–120
    [Google Scholar]
  11. Kloos W. E., Schleifer K.-H. 1986; Genus IV Staphylococcus. Bergeys Manual of Systematic Bacteriology 21013–1035 Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore, MD: Williams & Wilkins;
    [Google Scholar]
  12. Kloos W. E., Wolfshohl J. F. 1991; Staphylococcus cohnii subspecies : Staphylococcus cohnii subsp. cohnii subsp. nov. and Staphylococcus cohnii subsp. urealyticum subsp. nov. Int J Syst Bacteriol 41:284–289
    [Google Scholar]
  13. Kloos W. E., Schleifer K.-H., Gòtz R. 1991; The genus Staphylococcus. The Prokaryotes : a Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, 2. 21369–1420 Balows A., Triiper H. G., Dworkin M., Harder W., Schleifer K.-H. New York: Springer;
    [Google Scholar]
  14. Olsen G. J., Woese C. R. 1993; Ribosomal RNA: a key to phylogeny. FASEB J 7:113–123
    [Google Scholar]
  15. Palys T., Nakamura L. K., Cohan F. M. 1997; Discovery and classification of ecological diversity in the bacterial world : the role of DNA sequence data. Int J Syst Bacteriol A1:1145–1156
    [Google Scholar]
  16. Raué H. A., Musters W., Rutgers C. A., Van’t-Riet J., Pianta R. J. 1990; rRNA : from structure to function. The Ribosome : Structure, Function, and Evolution217–235 Hill W. E., Moore P. B., Dahlberg A., Schlessinger D., Garrett R. A., Warner J. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  17. Saitou N., Nei M. 1987; The neighbor-joining method : a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  18. Takahashi T., Kaneko M., Mori Y., Tsuji M., Kikuchi N., Hiramune T. 1997; Phylogenetic analyses of Staphylococcus based on the 16S rDNA sequence and assignment of clinical isolates from animals. J Vet Med Sci 59:775–783
    [Google Scholar]
  19. Tanasupawat S., Hashimoto Y., Ezaki T., Kozaki M., Komagata K. 1992; Staphylococcus piscifermentans sp. nov., from fermented fish in Thailand. Int J Syst Bacteriol 42:577–581
    [Google Scholar]
  20. Thomson-Carter F. M., Carter P. E., Pennington T. H. 1989; Differentiation of staphylococcal species and strains by ribosomal RNA gene restriction patterns. J Gen Microbiol 135:2093–2097
    [Google Scholar]
  21. Webster J. A., Bannerman T. L., Hubner R. J., Ballard D. N., Cole E. M., „ Bruce J. L., Fiedler F., Schubert K., Kloos W. E. 1994; Identification of the Staphylococcus sciuri species group with EcoKl fragments containing rRNA sequences and description of Staphylococcus vitulus sp. nov. Int J Syst Bacteriol 44:454–460
    [Google Scholar]
  22. Zakrzewska-Czerwinska J., Gaszewska-Mastalarz A., Lis B., Gamian A., Mordarski M. 1995; Staphylococcus pulvereri sp. nov., isolated from human and animal specimens. Int J Syst Bacteriol 45:169–172
    [Google Scholar]
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