Transfer of Bacillus lentimorbus and Bacillus popilliae to the genus Paenibacillus with emended descriptions of Paenibacillus lentimorbus comb. nov. and Paenibacillus popilliae comb. nov.
Almost complete 16S rRNA gene sequences were generated for the type strains of the obligate insect pathogens Bacillus lentimorbus and Bacillus popilliae and a second strain of Bacillus popilliae (NRRL B-4081) received as ‘Bacillus popilliae var. melolonthae’. A phylogenetic tree was constructed which grouped these strains into a well defined subcluster within the genus Paenibacillus. Bacillus popilliae NRRL B-4081 occupied an intermediate position between the type strains of Bacillus lentimorbus and Bacillus popilliae but with a marked clustering to the latter. The phylogenetic assignment of these strains to Paenibacillus is in contrast to earlier studies which placed these bacteria in the genus Bacillus, close to Bacillus subtilis. Indeed, the rRNA sequences generated in this study share less than 88% similarity to the deposited sequences for Bacillus popilliae ATCC 14706T and Bacillus lentimorbus ATCC 14707T. The results obtained by using different tree algorithms, bootstrap analysis, branch lengths and verification by signature nucleotide analysis supported the reclassif ication of these species in the genus Paenibacillus as Paenibacillus lentimorbus comb. nov. and Paenibacillus popilliae comb. nov.
AlexanderB.,
PriestF. G.1990; Numerical classification and identification of Bacillus sphaericus including some strains pathogenic for mosquito larvae. J Gen Microbiol 136:367–376
AltschulS. F.,
MaddenT. L.,
SchafferA. A.,
ZhangJ.,
ZhangZ.,
MillerW.,
LipmanD. J.1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3420
AshC.,
FarrowJ. A.,
Wall banksS.,
CollinsM. D.1991; Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small subunit ribosomal RNA sequences. Lett Appl Microbiol 13:202–206
AshC.,
PriestF. G.,
CollinsM. D.1993; Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Antonie Leeuwenhoek 64:253–260
de BarjacH.1981; Insect pathogens in the genus Bacillus. The Aerobic Endospore-forming Bacteria; Classification and Identification241–250GoodfellowM.,
BerkeleyR. C. W.
London: Academic Press;
CheshireF. R.,
CheyneW. W.1885; The pathogenic history and history under cultivation of a new bacillus (Balvei), the cause of a disease of the hive bee hitherto known as foulbrood. R Microsc Soc J Ser 115:581–601
ClaytonR. A.,
SuttonG.,
HinkleP. S.Jr,
BultC.,
FieldsC.1995; Intraspecific variation in small-subunit rRNA sequences in GenBank: why single sequences may not adequately represent prokaryotic taxa. Int J Syst Bacteriol 45:595–599
de SilvaS.,
PetterssonB.,
Aquino de MuroM.,
PriestF. G.1998; A DNA probe for the detection and identification of Bacillus sporothermodurans using the 16S 23S rDNA spacer region and phylogenetic analysis of some field isolates of Bacillus which form highly heat-resistant spores. Syst Appl Microbiol 21:398–407
GrayM. W.,
SankoffD.,
CedergrenR. J.1984; On the evolutionary descent of organisms and organelles: a global phylogeny based on a highly conserved structural core in small subunit ribosomal RNA. Nucleic Acids Res 12:5837–5852
GutellR. R.,
LarsenN.,
WoeseC. R.1994; Lessons from an evolving rRNA: 16S and 23S rRNA structures from a comparative perspective. Microbiol Rev 58:10–26
HultmanT.,
StàhlS.,
HomesE.,
UhlénM.1989; Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support. Nucleic Acids Res 17:4937–4946
MayberryR. W.,
LaneJ. R.1993; Sequential alkaline saponification/acid hydrolysis/esterification : one tube method with enhanced recovery of both cyclopropane and hydroxylated fatty acids. J Microbiol Methods 18:21–23
PetterssonB.,
JohanssonK.-E.,
UhlénM.1994; Sequence analysis of 16S rRNA from mycoplasmas by direct solid phase DNA sequencing. Appl Environ Microbiol 60:2456–2461
PetterssonB.,
LeitnerT.,
RonaghiM.,
BolskeG.,
UhlénM.,
JohanssonK.-E.1996a; Phylogeny of the Mycoplasma mycoides cluster as determined by sequence analysis of the 16S rRNA genes from the two rRNA operons. J Bacteriol 178:4131–4142
PetterssonB.,
LembkeF.,
HammerP.,
StackebrandtE.,
PriestF. G.1996b; Bacillus sporothermodurans a new species producing highly heat-resistant endospores. Int J Syst Bacteriol 46:759–764
PetterssonB.,
UhlénM.,
JohanssonK.-E.1996c; Phylogeny of some ruminant mycoplasmas based on 16S rRNA sequences and definition of a new cluster within the hominis group. Int J Syst Bacteriol 46:1093–1098
PetterssonB.,
BolskeG.,
ThiaucourtF.,
UhlénM.,
JohanssonK.-E.1998; Molecular evolution within the subspecies Mycoplasma capricolum subsp. capripneumoniae as judged from polymorphisms in the 16S rRNA genes. J Bacteriol 180:2350–2358
PriestF. G.1994; Systematics and ecology of Bacillus. Bacillus subtilis and Other Gram-positive Bacteria : Biochemistry, Physiology and Molecular Genetics3–16HochJ. A.,
SonensheinA. L.,
LosickR.
Washington, DC: American Society for Microbiology;
PriestF. G.,
GoodfellowM.,
ToddC.1981; The genus Bacillus a numerical analysis. The Aerobic Endospore-forming Bacteria: Classification and Identification91–103GoodfellowM.,
BerkeleyR. C. W.
London: Academic Press;
RippereK. E.,
TranM. T.,
YoustenA. A.,
HiluK. H.,
KleinM. G.1998; Bacilluspopilliae and Bacillus lentimorbus bacteria causing milky disease in Japanese beetles and related scarab larvae. Int J Syst Bacteriol 48:395–402
ShidaO.,
TakagiH.,
KadowakiK.,
KomagataK.1996; Proposal for two new genera, Brevibacillus gen. nov. and Aneurinibacillus gen. nov. Int J Syst Bacteriol 46:939–946
ShidaO.,
TakagiH.,
KadowakiK.,
NakamuraL. K.,
KomagataK.1997; Transfer of Bacillus alginolyticus Bacillus chondroitinus Bacillus glucanolyticus Bacillus kobensis and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus. Int J Syst Bacteriol 47:289–298
StahlyD. P.,
AndrewsR.,
YoustenA. A.1992; The genus Bacillussect pathogens. The Prokaryotes, 2.1697–1745BalowsA.,
TrüperH. G.,
DworkinM.,
HarderW.,
SchleiferK.-H.
New York: Springer;
Transfer of Bacillus lentimorbus and Bacillus popilliae to the genus Paenibacillus with emended descriptions of Paenibacillus lentimorbus comb. nov. and Paenibacillus popilliae comb. nov.