1887

Abstract

Almost complete 16S rRNA gene sequences were generated for the type strains of the obligate insect pathogens and and a second strain of (NRRL B-4081) received as ‘ var. ’. A phylogenetic tree was constructed which grouped these strains into a well defined subcluster within the genus . NRRL B-4081 occupied an intermediate position between the type strains of and but with a marked clustering to the latter. The phylogenetic assignment of these strains to is in contrast to earlier studies which placed these bacteria in the genus , close to . Indeed, the rRNA sequences generated in this study share less than 88% similarity to the deposited sequences for ATCC 14706 and ATCC 14707. The results obtained by using different tree algorithms, bootstrap analysis, branch lengths and verification by signature nucleotide analysis supported the reclassif ication of these species in the genus as comb. nov. and comb. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-49-2-531
1999-04-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/49/2/ijs-49-2-531.html?itemId=/content/journal/ijsem/10.1099/00207713-49-2-531&mimeType=html&fmt=ahah

References

  1. Alexander B., Priest F. G. 1990; Numerical classification and identification of Bacillus sphaericus including some strains pathogenic for mosquito larvae. J Gen Microbiol 136:367–376
    [Google Scholar]
  2. Altschul S. F., Madden T. L., Schaffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3420
    [Google Scholar]
  3. Ash C., Farrow J. A., Wall banks S., Collins M. D. 1991; Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small subunit ribosomal RNA sequences. Lett Appl Microbiol 13:202–206
    [Google Scholar]
  4. Ash C., Priest F. G., Collins M. D. 1993; Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Antonie Leeuwenhoek 64:253–260
    [Google Scholar]
  5. de Barjac H. 1981; Insect pathogens in the genus Bacillus. The Aerobic Endospore-forming Bacteria; Classification and Identification241–250 Goodfellow M., Berkeley R. C. W. London: Academic Press;
    [Google Scholar]
  6. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci USA 75:4801–4805
    [Google Scholar]
  7. Cheshire F. R., Cheyne W. W. 1885; The pathogenic history and history under cultivation of a new bacillus (Balvei), the cause of a disease of the hive bee hitherto known as foulbrood. R Microsc Soc J Ser 115:581–601
    [Google Scholar]
  8. Clayton R. A., Sutton G., Hinkle P. S. Jr, Bult C., Fields C. 1995; Intraspecific variation in small-subunit rRNA sequences in GenBank: why single sequences may not adequately represent prokaryotic taxa. Int J Syst Bacteriol 45:595–599
    [Google Scholar]
  9. Costilow R. N., Coulter W. H. 1971; Physiological studies of an oligosporogenous strain of Bacillus popilliae. Appl Microbiol 22:1076–1084
    [Google Scholar]
  10. de Silva S., Pettersson B., Aquino de Muro M., Priest F. G. 1998; A DNA probe for the detection and identification of Bacillus sporothermodurans using the 16S 23S rDNA spacer region and phylogenetic analysis of some field isolates of Bacillus which form highly heat-resistant spores. Syst Appl Microbiol 21:398–407
    [Google Scholar]
  11. Dingman D. W., Stahly D. P. 1983; Medium promoting sporulation of Bacillus larvae and metabolism of medium components. Appl Environ Microbiol 46:860–869
    [Google Scholar]
  12. Dutky S. R. 1940; Two new spore-forming bacteria causing milky disease in Japanese beetle larvae. J Agric Res 61:57–68
    [Google Scholar]
  13. Felsenstein J. 1993 PHYLIP (Phylogeny Inference Package) version 3.51c Seattle: Department of Genetics, University of Washington;
    [Google Scholar]
  14. Gordon R. E., Haynes W. C., Pang C. H.-N. 1973 The Genus Bacillus. Agriculture Handbook no. 427 Washington: DC-United States Department of Agriculture;
    [Google Scholar]
  15. Gray M. W., Sankoff D., Cedergren R. J. 1984; On the evolutionary descent of organisms and organelles: a global phylogeny based on a highly conserved structural core in small subunit ribosomal RNA. Nucleic Acids Res 12:5837–5852
    [Google Scholar]
  16. Gutell R. R., Larsen N., Woese C. R. 1994; Lessons from an evolving rRNA: 16S and 23S rRNA structures from a comparative perspective. Microbiol Rev 58:10–26
    [Google Scholar]
  17. Hultman T., Stàhl S., Homes E., Uhlén M. 1989; Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support. Nucleic Acids Res 17:4937–4946
    [Google Scholar]
  18. Hultman T., Bergh S., Moks T., Uhlén M. 1991; Bidirectional solid phase sequencing of in vz7roamplified plasmid DNA. BioTechniques 10:84–93
    [Google Scholar]
  19. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. Mammalian Protein Metabolism 321–132 Munro H. N. New York: Academic Press;
    [Google Scholar]
  20. Katznelson H. 1955; Bacillus apiarius n. sp. an aerobic spore-forming organism isolated from honeybee larvae. J Bacteriol 70:635–636
    [Google Scholar]
  21. Klein M. G., Kaya H. K. 1995; Bacillus and Serratia species for scarab control. Mem Inst Oswaldo Cruz 90:87–95
    [Google Scholar]
  22. Maidak B. L., Olsen G. J., Larsen N., Overbeek R., McCaughey M. J., Woese C. R. 1996; The Ribosomal Database Project (RDP). Nucleic Acids Res 24:82–85
    [Google Scholar]
  23. Mayberry R. W., Lane J. R. 1993; Sequential alkaline saponification/acid hydrolysis/esterification : one tube method with enhanced recovery of both cyclopropane and hydroxylated fatty acids. J Microbiol Methods 18:21–23
    [Google Scholar]
  24. Pettersson B., Johansson K.-E., Uhlén M. 1994; Sequence analysis of 16S rRNA from mycoplasmas by direct solid phase DNA sequencing. Appl Environ Microbiol 60:2456–2461
    [Google Scholar]
  25. Pettersson B., Leitner T., Ronaghi M., Bolske G., Uhlén M., Johansson K.-E. 1996a; Phylogeny of the Mycoplasma mycoides cluster as determined by sequence analysis of the 16S rRNA genes from the two rRNA operons. J Bacteriol 178:4131–4142
    [Google Scholar]
  26. Pettersson B., Lembke F., Hammer P., Stackebrandt E., Priest F. G. 1996b; Bacillus sporothermodurans a new species producing highly heat-resistant endospores. Int J Syst Bacteriol 46:759–764
    [Google Scholar]
  27. Pettersson B., Uhlén M., Johansson K.-E. 1996c; Phylogeny of some ruminant mycoplasmas based on 16S rRNA sequences and definition of a new cluster within the hominis group. Int J Syst Bacteriol 46:1093–1098
    [Google Scholar]
  28. Pettersson B., Bolske G., Thiaucourt F., Uhlén M., Johansson K.-E. 1998; Molecular evolution within the subspecies Mycoplasma capricolum subsp. capripneumoniae as judged from polymorphisms in the 16S rRNA genes. J Bacteriol 180:2350–2358
    [Google Scholar]
  29. Priest F. G. 1994; Systematics and ecology of Bacillus. Bacillus subtilis and Other Gram-positive Bacteria : Biochemistry, Physiology and Molecular Genetics3–16 Hoch J. A., Sonenshein A. L., Losick R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  30. Priest F. G., Goodfellow M., Todd C. 1981; The genus Bacillus a numerical analysis. The Aerobic Endospore-forming Bacteria: Classification and Identification91–103 Goodfellow M., Berkeley R. C. W. London: Academic Press;
    [Google Scholar]
  31. Priest F. G., Goodfellow M., Todd C. 1988; A numerical classification of the genus Bacillus. J Gen Microbiol 134:1847–1882
    [Google Scholar]
  32. Rippere K. E., Tran M. T., Yousten A. A., Hilu K. H., Klein M. G. 1998; Bacilluspopilliae and Bacillus lentimorbus bacteria causing milky disease in Japanese beetles and related scarab larvae. Int J Syst Bacteriol 48:395–402
    [Google Scholar]
  33. Rudner R., Martsinkevich O., Leung W., Jarvis E. D. 1998; Classification and genetic characterization of pattern-forming bacilli. Mol Microbiol 27:687–703
    [Google Scholar]
  34. Saitou N., Nei M. 1987; The neighbor-joining method : a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  35. Shida O., Takagi H., Kadowaki K., Komagata K. 1996; Proposal for two new genera, Brevibacillus gen. nov. and Aneurinibacillus gen. nov. Int J Syst Bacteriol 46:939–946
    [Google Scholar]
  36. Shida O., Takagi H., Kadowaki K., Nakamura L. K., Komagata K. 1997; Transfer of Bacillus alginolyticus Bacillus chondroitinus Bacillus glucanolyticus Bacillus kobensis and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus. Int J Syst Bacteriol 47:289–298
    [Google Scholar]
  37. Smith S. 1992 GDE (Genetic Data Environment) version 2.2 Ann Arbor, MI: Millipore Imaging Systems;
    [Google Scholar]
  38. Stahly D. P., Andrews R., Yousten A. A. 1992; The genus Bacillussect pathogens. The Prokaryotes, 2.1697–1745 Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. New York: Springer;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-49-2-531
Loading
/content/journal/ijsem/10.1099/00207713-49-2-531
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error