Analysis of PCR products of 16S rDNA of 680 isolates from Mediterranean Sea mesocosm experiments with taxon-specific 16S rDNA oligonucleotides revealed that 262 isolates belonged to the α subclass of the class . Partial 16S rDNA sequence analysis of selected isolates and oligonucleotide probing with a 16S rDNA probe affiliated 33 strains to the genus . Analysis of the Haelll digest pattern of 16S rDNA revealed the presence of two groups; while 30 strains showed a pattern identical with that obtained for DSM 10014, a second group of three strains had a unique pattern that was different from that of the type strain. Five isolates of group 1 and one isolates of group 2, strain CH-B427, were selected for detailed taxonomic analysis. All six isolates closely resembled the type strain DSM 10014in physiological reactions. However, strain CH-B427differed quantitatively in the composition of fatty acids from DSM 10014and showed only 98·2% 16S rDNA sequence similarity with strain DSM 10014. DNA-DNA reassociation value obtained for strains DSM 10014and CH-B427revealed 46 % similarity. Based on the results of DNA-DNA reassociation and discrete differences in the nucleotide composition of 16S rDNA, a new species of the genus is proposed, designated sp. nov., the type strain being strain CH-B427(= DSM 12244).


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