1887

Abstract

Three strains of a previously undescribed catalase-positive non-lipophilic coryneform bacterium isolated from human clinical specimens were characterized by phenotypic and molecular taxonomic methods. Morphologically the unknown bacterium consisted of pleomorphic rods, some of which displayed bulges/knobs at their ends. All three strains were similar in that they produced acid from fructose, glucose, maltose and sucrose and were urease-positive. Chemotaxonomic investigations revealed the presence of -diaminopimelic acid and short-chain mycolic acids consistent with the genus Comparative 16S rRNA gene sequencing showed that the three strains are genealogically highly related and constitute a new subline within the genus displaying >3% sequence divergence with recognized species. The unknown bacterium was distinguished from currently validly published species by phenotypic tests, including electrophoretic analysis of whole-cell proteins. Based on phylogenetic and phenotypic evidence, it is proposed that the unknown bacterium from clinical specimens be classified as sp. nov. The type strain is CCUG 36622.

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1999-04-01
2022-10-01
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References

  1. Bernard K. A., Bellefeuille M., Evans E. P. 1991; Cellular fatty acid composition as an adjunct to the identification of asporogenous, aerobic gram-positive rods. J Clin Microbiol 29:83–89
    [Google Scholar]
  2. Bernard K. A., Cooper C., Johnson W. 1997; Prevalence of anaerobes referred to the Canadian National Reference Centre from 1984 to 1996. Clin Infect Dis 25:S241–S243
    [Google Scholar]
  3. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12:387–395
    [Google Scholar]
  4. Felsenstein J. 1989; phylip - phylogeny inference package (version 3.2). Cladistics 5:164–166
    [Google Scholar]
  5. Funke G., Bernard K. A., Bucher C., Pfyffer G. E., Collins M. D. 1995a; Corynebacterium glucuronolyticum sp. nov. isolated from male patients with genitourinary infections. Med Microbiol Lett 4:204–215
    [Google Scholar]
  6. Funke G., Lawson P. A., Collins M. D. 1995b; Heterogeneity within Centers for Disease Control and Prevention coryneform group ANF-1-like bacteria and description of Corynebacterium auris sp. nov. Int J Syst Bacteriol 45:735–739
    [Google Scholar]
  7. Funke G., Hutson R. A., Hilleringmann M., Heizmann W. R., Collins M. D. 1997a; Corynebacterium lipophiloflavum sp. nov. isolated from a patient with bacterial vaginosis. FEMS Microbiol Lett 150:219–224
    [Google Scholar]
  8. Funke G., Lawson P. A., Collins M. D. 1997b; Corynebacterium mucifaciens sp. nov., an unusual species from human clinical material. Int J Syst Bacteriol 47:952–957
    [Google Scholar]
  9. Funke G., Pascual Ramos C., Collins M. D. 1997c; Corynebacterium coyleae sp. nov., isolated from human clinical specimens. Int J Syst Bacteriol 47:92–96
    [Google Scholar]
  10. Funke G., Von Graevenitz A., Clarridge J. E. III, Bernard K. A. 1997d; Clinical microbiology of coryneform bacteria. Clin Microbiol Rev 10:125–159
    [Google Scholar]
  11. Funke G., Lawson P. A., Collins M. D. 1998; Corynebacterium riegelii sp. nov., an unusual species isolated from female patients with urinary tract infections. J Clin Microbiol 36:624–627
    [Google Scholar]
  12. Garvie E. I. 1978; Streptococcus raffinolactis (Orla-Jensen and Hansen): a group N streptococcus found in raw milk. Int J Syst Bacteriol 28:190–193
    [Google Scholar]
  13. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. 1980; Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188:221–234
    [Google Scholar]
  14. Pot B., Vandamme P., Kersters K. 1994; Analysis of electrophoretic whole-organism protein fingerprints. Modern Microbiol Methods. Chemical Methods in Prokaryotic Systematics,493–521 Goodfellow M., O’Donnell A. G. Chichester: Wiley;
    [Google Scholar]
  15. Riegel P., de Briel D., Prévost G., Jehl F., Monteil H., Minck R. 1993a; Taxonomic study of Corynebacterium group ANF-1 strains: proposal of Corynebacterium afermentans sp. nov. containing the subspecies C. afermentans subsp. afermentans subsp. nov. and C. afermentans subsp. lipophilum subsp. nov. Int J Syst Bacteriol 43:286–292
    [Google Scholar]
  16. Riegel P., de Briel D., Prévost G., Jehl F., Monteil H. 1993b; Proposal of Corynebacterium propinquum sp. nov. for Corynebacterium group ANF-3 strains. FEMS Microbiol Lett 113:
    [Google Scholar]
  17. Riegel P., Ruimy R., de Briel D., Prévost G., Jehl F., Bimet F., Christen R., Monteil H. 1995a; Corynebacterium argentoratense sp. nov., from human throat. Int J Syst Bacteriol 45:533–537
    [Google Scholar]
  18. Riegel P., Ruimy R., de Briel D., Prévost G., Jehl F., Christen R., Monteil H. 1995b; Genomic diversity and phylogenetic relationships among lipid-requiring diphtheroids from humans and characterization of Corynebacterium macginleyi sp. nov. Int J Syst Bacteriol 45:128–133
    [Google Scholar]
  19. Riegel P., Heller R., Provost G., Jehl F., Monteil H. 1997a; Corynebacterium durum sp. nov., from human clinical specimens. Int J Syst Bacteriol 47:1107–1111
    [Google Scholar]
  20. Riegel P., Ruimy R., Renaud F. N. R., Freney J., Prévost G., Jehl F., Christen R., Monteil H. 1997b; Corynebacterium singulare sp. nov., a new species for urease-positive strains related to Corynebacterium minutissimum . Int J Syst Bacteriol 47:
    [Google Scholar]
  21. Staneck J. L., Roberts G. D. 1974; Simplified approach to the identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
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