1887

Abstract

Unusual growth characteristics of a spoilage yeast, originally isolated from spoiled wholeorange drink and previously identified as , prompted careful re-examination of its taxonomic position. Small-subunit rRNA gene sequences were determined for this strain and for four other strains also originally described as . but which, in contrast to other strains of this species, grew poorly or not at all under aerobic conditions with agitation, failed to grow in the presence of 1 % acetic acid and failed to grow at 30 °C. Comparative sequence analysis revealed that these strains represented a phylogenetically distinct taxon closely related to, but distinct from, . and . Furthermore, sequence analysis of the internal transcribed spacer (ITS) region showed that, while all five strains had identical ITS2 sequences, they could be subdivided into two groups based on ITS1 sequences. Despite such minor inter-strain sequence variation, these yeasts could readily be distinguished from all other currently described species by using ITS sequences. On the basis of the phylogenetic results presented, a new species comprising the five strains, sp. nov., is described and supporting physiological data are discussed, including a demonstration that growth of this species is particularly sensitive to the presence of oxygen. The type strain of . is NCYC D2627.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-49-1-319
1999-01-01
2022-05-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/49/1/ijs-49-1-319.html?itemId=/content/journal/ijsem/10.1099/00207713-49-1-319&mimeType=html&fmt=ahah

References

  1. Barnett J. A., Payne R. W., Yarrow D. 1990 Yeasts: Characteristics and Identification, 2nd. Cambridge: Cambridge University Press;
    [Google Scholar]
  2. Cai J., Roberts I. N., Collins M. D. 1996; Phylogenetic relationships among members of the ascomycetous yeast genera Brettanomyces, Debaryomyces, Dekkera, and Kluyveromyces deduced by small-subunit rRNA gene sequences. Int J Syst Bacteriol 46:542–549
    [Google Scholar]
  3. Davenport R. R. 1975 The distribution of yeasts and yeast-like organisms in an English vineyard PhD thesis, University of Bristol;
    [Google Scholar]
  4. Davenport R. R. 1981; Yeasts and yeast-like organisms. In Smith’s Introduction to Industrial Mycology, 7th. Onions A. H. S., Allsopp D., Eggins H. O. W. London: Edward Arnold;
    [Google Scholar]
  5. Davenport R. R. 1996; Forensic microbiology for soft drinks business. Soft Drinks Manag Int April 1996:34–35
    [Google Scholar]
  6. Drubin D. G. 1991; Development of cell polarity in budding yeast. Cell 65:1093–1096
    [Google Scholar]
  7. Farris G. A., Fatichenti F., Deiana P. 1981; Microbiological characterization of Sardinian honeys. Tecnol Aliment 4:18–23
    [Google Scholar]
  8. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791
    [Google Scholar]
  9. Felsenstein J. 1993; phylip: phylogenetic inference package, version 3.5. University of Washington; Seattle, WA, USA:
    [Google Scholar]
  10. Feng D. F., Doolittle R. F. 1987; Progressive sequence alignment as a prerequisite to correct phylogenetic trees. J Mol Evol 25:351–360
    [Google Scholar]
  11. Genetics Computer Group 1991 Program Manual for the GCG Package, version 7 Madison: WI: Genetics Computer Group;
    [Google Scholar]
  12. Grinbaum A., Ashkenazi I., Treister G., Goldschmied-Requven A., Block C. S. 1994; Exploding bottles: eye injury due to yeast fermentation of an uncarbonated soft drink. Br J Ophthalmol 78:883
    [Google Scholar]
  13. Hammond S. M., Carr J. G. 1976; The antimicrobial activity of SO2-with particular reference to fermented and non-fermented fruit juices. In Inhibition and Inactivation of Vegetative Microbes89–110 Skinner F. A., Hugo W. B. London: Academic Press;
    [Google Scholar]
  14. Ingram I. 1960; Studies on benzoate-resistant yeasts. Acta Microbiol 7:95–105
    [Google Scholar]
  15. James S.A., Collins M. D., Roberts I. N. 1994; Genetic interrelationship among species of the genus Zygosaccharo-myces as revealed by small-subunit rRNA gene sequences. Yeast 10:871–881
    [Google Scholar]
  16. James S. A., Collins M. D., Roberts I. N. 1996; Use of an rRNA internal transcribed spacer region to distinguish phylo-genetically closely related species of the genera Zygo-saccharomyces and Torulaspora . Int J Syst Bacteriol 46:189–194
    [Google Scholar]
  17. James S. A., Cai J., Roberts I. N., Collins M. D. 1997; A phylogenetic analysis of the genus Saccharomyces based on 18S rRNA gene sequences : description of Saccharomyces kunashi-rensis sp. nov. and Saccharomyces martiniae sp. nov. Int J Syst Bacteriol 47:453–460
    [Google Scholar]
  18. James S. A., Roberts I. N., Collins M. D. 1998; Phylogenetic heterogeneity of the genus Williopsis as revealed by 18S rRNA gene sequences. Int J Syst Bacteriol 48:591–596
    [Google Scholar]
  19. Kurtzman C. P. 1990; DNA relatedness among species of the genus Zygosaccharomyces. Yeast 6:213–219
    [Google Scholar]
  20. Lemontt J. F., MacKay V. L. 1977; A pleiotropic mutant of yeast expressing the mating specific ‘schmoo’ morphology during vegetative growth in the absence of exogenous mating hormone. Genetics 86:s38
    [Google Scholar]
  21. Mankin A. S., Skryabin K. G., Rubtsov P. M. 1986; Identification often additional nucleotides in the primary structure of yeast 18S rRNA. Gene 44:143–145
    [Google Scholar]
  22. Munitis M.T., Cabrera E., Rodriguez-Navarro A. 1976; An obligate osmophilic yeast from honey. Appl Environ Microbiol 32:320–323
    [Google Scholar]
  23. Neefs J. M., Van der Peer Y., De Rijk P., Goris A., De Wachter R. 1991; Compilation of small ribosomal subunit RNA sequences. Nucleic Acids Res 19: Suppl 1987–2015
    [Google Scholar]
  24. Neves L., Pampulha M. E., Loureiro-Dias M. C. 1994; Resistance of food spoilage yeasts to sorbic acid. Lett Appl Microbiol 19:8–11
    [Google Scholar]
  25. Pitt J. I. 1974; Resistance of some food spoilage yeasts to preservatives. Food Technol Aust 6:238–241
    [Google Scholar]
  26. Pitt J. I., Hocking A. D. 1985 Fungi and Food Spoilage Sydney: Academic Press;
    [Google Scholar]
  27. Saitou N., Nei M. 1987; The neighbor-joining method : a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  28. Sand F. E. M. J., van Grimsven A. M. 1976; Comparison between the yeast flora of Middle Eastern and Western European soft drinks. Antonie Leeuwenhoek 42:523–532
    [Google Scholar]
  29. Scarr M. P., Rose D. 1966; Study of osmophilic yeasts producing invertase. J Gen Microbiol 45:9–16
    [Google Scholar]
  30. Thomas D. S., Davenport R. R. 1985; Zygosaccharomyces bailii : a profile of characteristics and spoilage activities. Food Microbiol 2:157–169
    [Google Scholar]
  31. Tilbury R. H. 1980; Xerotolerant yeasts at high sugar concentrations. In Microbiol Growth and Survival in Extremes of Environment pp. 103–128 Gould G. W., Corry J. E. L. London: Academic Press;
    [Google Scholar]
  32. van der Walt J. P., Johannsen E. 1975; The genus Torulaspora Lindner. CSIR Res Rep 325:1–23
    [Google Scholar]
  33. van der Walt J. P., Yarrow D. 1984; Methods for isolation, maintenance, classification of yeasts. In The Yeasts - a Taxo-nomic Study, 3rd.45–104 Kregervan Rij N. J. W. Amsterdam: Elsevier;
    [Google Scholar]
  34. Warth A. D. 1977; Mechanism of resistance of Saccharomyces bailii to benzoic, sorbic and other weak acids used as food preservatives. J Appl Bacteriol 43:215–230
    [Google Scholar]
  35. Warth A. D. 1989; Relationships among cell size, membrane permeability, and preservative resistance in yeast species. Appl Environ Microbiol 55:2995–2999
    [Google Scholar]
  36. Yarrow D. 1984; Zygosaccharomyces Barker. In The Yeasts-a Taxonomic Study, 3rd.449–465 Kreger-van Rij N. J. W. Amsterdam: Elsevier;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-49-1-319
Loading
/content/journal/ijsem/10.1099/00207713-49-1-319
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error